Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Holliday junction ATP-dependent DNA helicase RuvB

Gene

ruvB

Organism
Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi69 – 768ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, SOS response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCNEC381666:GJUJ-497-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Holliday junction ATP-dependent DNA helicase RuvBUniRule annotation (EC:3.6.4.12UniRule annotation)
Gene namesi
Name:ruvBUniRule annotation
Ordered Locus Names:H16_A0497
OrganismiCupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
Taxonomic identifieri381666 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
Proteomesi
  • UP000008210 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 352352Holliday junction ATP-dependent DNA helicase RuvBPRO_1000001453Add
BLAST

Interactioni

Subunit structurei

Forms a complex with RuvA.UniRule annotation

Protein-protein interaction databases

STRINGi381666.H16_A0497.

Structurei

3D structure databases

ProteinModelPortaliQ0KEC4.
SMRiQ0KEC4. Positions 29-340.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CKJ. Bacteria.
COG2255. LUCA.
HOGENOMiHOG000218623.
KOiK03551.
OMAiYEPYLMQ.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00016. DNA_helic_RuvB. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR004605. DNA_helicase_Holl-junc_RuvB.
IPR008823. DNA_helicase_Holl-junc_RuvB_C.
IPR027417. P-loop_NTPase.
IPR008824. RuvB_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF05491. RuvB_C. 1 hit.
PF05496. RuvB_N. 1 hit.
[Graphical view]
ProDomiPD005323. DNA_helicase_Holl-junc_RuvB_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00635. ruvB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0KEC4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIETDKLAAP ARVIAATPAS SQEEAFERAL RPKLLDEYVG QEKVRGQLDI
60 70 80 90 100
FMHAARNRRE ALDHVLLFGP PGLGKTTLAH IIAREMGVNL RQTSGPVLER
110 120 130 140 150
PGDLAALLTN LEANDVLFID EIHRLSPVVE EILYPALEDY QIDIMIGEGP
160 170 180 190 200
AARSVKLDLQ PFTLVGATTR AGMLTNPLRD RFGIVARLEF YTADELARIV
210 220 230 240 250
TRSAQLLHAR IDPQGALEIA RRARGTPRIA NRLLRRVRDY AEVKGDGNIT
260 270 280 290 300
RAMADAALAM LDVDSVGFDL MDRKLLEAVL HKFGGGPVGV DNLAAAIGEE
310 320 330 340 350
RDTIEDVLEP YLIQQGYLQR TPRGRVATAA AYRHFGLANP QGSGASELFG

DA
Length:352
Mass (Da):38,511
Last modified:October 3, 2006 - v1
Checksum:iB933903EC59281DB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM260479 Genomic DNA. Translation: CAJ91647.1.
RefSeqiWP_010813892.1. NC_008313.1.

Genome annotation databases

EnsemblBacteriaiCAJ91647; CAJ91647; H16_A0497.
GeneIDi4249070.
KEGGireh:H16_A0497.
PATRICi35230385. VBIRalEut6770_0862.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM260479 Genomic DNA. Translation: CAJ91647.1.
RefSeqiWP_010813892.1. NC_008313.1.

3D structure databases

ProteinModelPortaliQ0KEC4.
SMRiQ0KEC4. Positions 29-340.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi381666.H16_A0497.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ91647; CAJ91647; H16_A0497.
GeneIDi4249070.
KEGGireh:H16_A0497.
PATRICi35230385. VBIRalEut6770_0862.

Phylogenomic databases

eggNOGiENOG4105CKJ. Bacteria.
COG2255. LUCA.
HOGENOMiHOG000218623.
KOiK03551.
OMAiYEPYLMQ.

Enzyme and pathway databases

BioCyciCNEC381666:GJUJ-497-MONOMER.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00016. DNA_helic_RuvB. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR004605. DNA_helicase_Holl-junc_RuvB.
IPR008823. DNA_helicase_Holl-junc_RuvB_C.
IPR027417. P-loop_NTPase.
IPR008824. RuvB_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF05491. RuvB_C. 1 hit.
PF05496. RuvB_N. 1 hit.
[Graphical view]
ProDomiPD005323. DNA_helicase_Holl-junc_RuvB_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00635. ruvB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRUVB_CUPNH
AccessioniPrimary (citable) accession number: Q0KEC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 3, 2006
Last modified: September 7, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.