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Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi7MagnesiumUniRule annotation1
Metal bindingi67MagnesiumUniRule annotation1
Metal bindingi139MagnesiumUniRule annotation1
Metal bindingi142MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Nuclease
Biological processDNA damage, DNA recombination, DNA repair
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvCUniRule annotation (EC:3.1.22.4UniRule annotation)
Alternative name(s):
Holliday junction nuclease RuvCUniRule annotation
Holliday junction resolvase RuvCUniRule annotation
Gene namesi
Name:ruvCUniRule annotation
Ordered Locus Names:H16_A0500
OrganismiCupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
Taxonomic identifieri381666 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
Proteomesi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000028071 – 181Crossover junction endodeoxyribonuclease RuvCAdd BLAST181

Proteomic databases

PRIDEiQ0KEC1

Interactioni

Protein-protein interaction databases

STRINGi381666.H16_A0500

Structurei

3D structure databases

ProteinModelPortaliQ0KEC1
SMRiQ0KEC1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105NHU Bacteria
COG0817 LUCA
HOGENOMiHOG000012181
KOiK01159
OMAiGLTRCGI

Family and domain databases

Gene3Di3.30.420.10, 1 hit
HAMAPiMF_00034 RuvC, 1 hit
InterProiView protein in InterPro
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
IPR020563 X-over_junc_endoDNase_Mg_BS
IPR002176 X-over_junc_endoDNase_RuvC
PANTHERiPTHR30194 PTHR30194, 1 hit
PfamiView protein in Pfam
PF02075 RuvC, 1 hit
PRINTSiPR00696 RSOLVASERUVC
SUPFAMiSSF53098 SSF53098, 1 hit
TIGRFAMsiTIGR00228 ruvC, 1 hit
PROSITEiView protein in PROSITE
PS01321 RUVC, 1 hit

Sequencei

Sequence statusi: Complete.

Q0KEC1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRILGIDPGL RTTGFGVIEK HGNKLAYVAS GTIKSDGNST LPERLKTLYD
60 70 80 90 100
GISEVSRTYA PDCAAIEKVF VNVNPQSTLL LGQARGAAIC GLVGYGLPVF
110 120 130 140 150
EYTALQLKVA VVGYGRANKA QVQEMVTRLL MLPGQPGSDA ADALGVAICH
160 170 180
ANGSDTLGTL SGLAPDLVRK GMRVRRGRLV G
Length:181
Mass (Da):18,997
Last modified:October 3, 2006 - v1
Checksum:i3891B23AD62FEA77
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM260479 Genomic DNA Translation: CAJ91650.1
RefSeqiWP_010813889.1, NC_008313.1

Genome annotation databases

EnsemblBacteriaiCAJ91650; CAJ91650; H16_A0500
KEGGireh:H16_A0500

Similar proteinsi

Entry informationi

Entry nameiRUVC_CUPNH
AccessioniPrimary (citable) accession number: Q0KEC1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 3, 2006
Last modified: May 23, 2018
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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