Q0KE35 (PLSY_CUPNH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol-3-phosphate acyltransferase

Alternative name(s):
Acyl-PO4 G3P acyltransferase
Acyl-phosphate--glycerol-3-phosphate acyltransferase
G3P acyltransferase
Short name=GPAT
EC=2.3.1.n3
Lysophosphatidic acid synthase
Short name=LPA synthase

Gene names

Name:	plsY
Ordered Locus Names:	H16_A0588

Organism

Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha) [Complete proteome] [HAMAP]

Taxonomic identifier

381666 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus › Cupriavidus necator

Protein attributes

Sequence length	202 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of an acyl group from acyl-phosphate (acyl-PO4) to glycerol-3-phosphate (G3P) to form lysophosphatidic acid (LPA). This enzyme utilizes acyl-phosphate as fatty acyl donor, but not acyl-CoA or acyl-ACP By similarity. HAMAP-Rule MF_01043
Catalytic activity	Acyl-phosphate + sn-glycerol 3-phosphate = 1-acyl-sn-glycerol 3-phosphate + phosphate. HAMAP-Rule MF_01043
Pathway	Lipid metabolism; phospholipid metabolism. HAMAP-Rule MF_01043
Subunit structure	Probably interacts with PlsX By similarity. HAMAP-Rule MF_01043
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP-Rule MF_01043.
Sequence similarities	Belongs to the PlsY family.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism Phospholipid biosynthesis Phospholipid metabolism
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	phospholipid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	acyl-phosphate glycerol-3-phosphate acyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 202	202	Glycerol-3-phosphate acyltransferase HAMAP-Rule MF_01043		PRO_1000064212
Regions
	Transmembrane	2 – 22	21	Helical; Potential
	Transmembrane	80 – 100	21	Helical; Potential
	Transmembrane	119 – 139	21	Helical; Potential
	Transmembrane	158 – 178	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q0KE35 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: 7622111C86F5CA03	FASTA	202	21,263
	10 20 30 40 50 60 MANLLFALAA YLIGSVSFAV VVSKLMGLPD PHSYGSGNPG ATNVLRTGNK KAAILTLIGD 70 80 90 100 110 120 ALKGWLAVWL AARFGPAYGL NETGLAMVAL AVFLGHLFPV YHRFAGGKGV ATAAGILLAI 130 140 150 160 170 180 DPILGLGTLA TWLIIAFFFR YSSLAALVAA IFAPFFHVLM NGVDVMTGAI FVISVLLIAR 190 200 HRQNIAKLLA GKESRIGEKK KV

References

[1]

"Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia eutropha H16." Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I., Gottschalk G., Steinbuechel A., Friedrich B., Bowien B. Nat. Biotechnol. 24:1257-1262(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM260479 Genomic DNA. Translation: CAJ91736.1.
RefSeq	YP_725104.1. NC_008313.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	381666.H16_A0588.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAJ91736; CAJ91736; H16_A0588.
GeneID	4247755.
KEGG	reh:H16_A0588.
PATRIC	35230575. VBIRalEut6770_0956.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0344.
HOGENOM	HOG000283806.
KO	K08591.
OMA	WRHRGNL.
ProtClustDB	PRK00220.
Enzyme and pathway databases
BioCyc	CNEC381666:GJUJ-587-MONOMER.
UniPathway	UPA00085.
Family and domain databases
HAMAP	MF_01043. PlsY.
InterPro	IPR003811. G3P_acylTferase. [Graphical view]
Pfam	PF02660. G3P_acyltransf. 1 hit. [Graphical view]
TIGRFAMs	TIGR00023. TIGR00023. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLSY_CUPNH

Accession

Primary (citable) accession number: Q0KE35

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 3, 2006
Last modified:	May 1, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program