Q0KDH9 (AROA_CUPNH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferase
EC=2.5.1.19

Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase
Short name=EPSP synthase
Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	H16_A0795

Organism

Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha) [Complete proteome] [HAMAP]

Taxonomic identifier

381666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	434 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity. HAMAP MF_00210
Subcellular location	Cytoplasm By similarity HAMAP MF_00210.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 434	434	3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_1000012463

Sequences

	Sequence		Length	Mass (Da)
	Q0KDH9 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: 870648BBE540D7F2	FASTA	434	46,416
	10 20 30 40 50 60 MEHLTLGPLT RANGTVRLPG SKSISNRVLL LAALATGETR VRDLLDSDDT RVMLQALRTL 70 80 90 100 110 120 GVAWRQEGDD YIVTGAGGNF PNKSAELFMG NAGTAIRPLT AALALQGGNY KLSGVPRMHE 130 140 150 160 170 180 RPIGDLVDGL RQVGAVIDYL GNEGFPPLHI QPAGIRIDAP IRVRGDVSSQ FLTALLMSLP 190 200 210 220 230 240 MAQSDSGRIE IEVVGELISK PYIEITLNLL ARFGIEIERQ GWERFVLPAG AAYRSPGEIF 250 260 270 280 290 300 VEGDASSASY FLAAGAIGGG PVRVEGVGMA SIQGDVRFAE ALNRMGANVM AGDNWIEVRG 310 320 330 340 350 360 TERDDGRLHG IELDCNHIPD AAMTLAVAAL FAEGTTTLTN IASWRVKETD RIAAMATELR 370 380 390 400 410 420 KLGAVVEEGA DYLRVTPPQP WQTPADGIGT YDDHRMAMCF SLAAFGPLPV RINDPGCVAK 430 TFPDYFSVFA GVTG

References

[1]

"Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia eutropha H16." Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I., Gottschalk G., Steinbuechel A., Friedrich B., Bowien B. Nat. Biotechnol. 24:1257-1262(2006) [PubMed: 16964242] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM260479 Genomic DNA. Translation: CAJ91942.1.
RefSeq	YP_725310.1. NC_008313.1.
3D structure databases
ProteinModelPortal	Q0KDH9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0KDH9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4247116.
GenomeReviews	Gene locus H16_A0795 in contig AM260479_GR.
KEGG	reh:H16_A0795.
PATRIC	35230997. VBIRalEut6770_1166.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0128.
HOGENOM	HBG646626.
OMA	GADIEWG.
PhylomeDB	Q0KDH9.
ProtClustDB	PRK02427.
Enzyme and pathway databases
BioCyc	REUT381666:H16_A0795-MONOMER.
Family and domain databases
HAMAP	MF_00210. EPSP_synth. [Tree]
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR006264. EPSP_synthase. IPR023193. EPSP_synthase_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
KO	K00800.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01356. AroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_CUPNH

Accession

Primary (citable) accession number: Q0KDH9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 3, 2006
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program