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Reviewed, UniProtKB/Swiss-Prot Q0KDH0 (HLDD_RALEH)

Last modified June 16, 2009. Version 27. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    ADP-L-glycero-D-manno-heptose-6-epimerase
    EC=5.1.3.20
Alternative name(s):
    ADP-L-glycero-beta-D-manno-heptose-6-epimerase
      Short name=ADP-glyceromanno-heptose 6-epimerase
      Short name=ADP-hep 6-epimerase
      Short name=AGME
Gene names
Name: hldD
Ordered Locus Names: H16_A0804
OrganismRalstonia eutropha (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)) [Complete proteome] [HAMAP]
Taxonomic identifier381666 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

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Protein attributes

Sequence length331 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Catalyzes the interconversion between ADP-D-glycero-beta-D-manno-heptose and ADP-L-glycero-beta-D-manno-heptose via an epimerization at carbon 6 of the heptose By similarity.

Catalytic activity

ADP-D-glycero-D-manno-heptose = ADP-L-glycero-D-manno-heptose. HAMAP MF_01601

Cofactor

Binds 1 NADP+ per subunit By similarity.

Pathway

Nucleotide-sugar biosynthesis; ADP-L-glycero-beta-D-manno-heptose biosynthesis; ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate: step 4/4. HAMAP MF_01601

Subunit structure

Homopentamer By similarity.

Domain

Contains a large N-terminal NADP-binding domain, and a smaller C-terminal substrate-binding domain By similarity.

Sequence similarities

Belongs to the sugar epimerase family. HldD subfamily.

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Ontologies

Keywords
   Biological processCarbohydrate metabolism
   LigandNADP
   Molecular functionIsomerase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processcarbohydrate metabolic process

Inferred from electronic annotation. Source: HAMAP

cellular metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular functionADP-glyceromanno-heptose 6-epimerase activity

Inferred from electronic annotation. Source: HAMAP

NADP or NADPH binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 331331ADP-L-glycero-D-manno-heptose-6-epimerase HAMAP MF_01601
PRO_1000069365

Regions

Nucleotide binding11 – 122NADP By similarity
Nucleotide binding32 – 332NADP By similarity
Nucleotide binding75 – 795NADP By similarity
Region200 – 2034Substrate binding By similarity

Sites

Active site1391Proton acceptor By similarity
Active site1771Proton acceptor By similarity
Binding site391NADP By similarity
Binding site541NADP By similarity
Binding site921NADP By similarity
Binding site1431NADP By similarity
Binding site1681Substrate By similarity
Binding site1691NADP; via amide nitrogen By similarity
Binding site1771NADP By similarity
Binding site1791Substrate; via carbonyl oxygen By similarity
Binding site1861Substrate By similarity
Binding site2131Substrate By similarity
Binding site2921Substrate By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q0KDH0-1 [UniParc].

Last modified October 3, 2006. Version 1.
Checksum: 4ED4171C4F798A5E

FASTA33137,428
        10         20         30         40         50         60 
MTIIVTGAAG FIGSNLVKGL NDRGETNVIA VDNLTRADKF HNLVDCEISD YLDKQDFLAR 

        70         80         90        100        110        120 
FARGEFGKVR AVFHEGACSD TMETDGRYMM ENNYRYTLSL MESCLEQGTQ FLYASSAATY 

       130        140        150        160        170        180 
GASQVFREDR EFERPLNVYG YSKFLFDQIV RRRLPSALSQ IVGFRYFNVY GPRETHKGRM 

       190        200        210        220        230        240 
ASVAFHNFNQ FRADGTVKLF GEYGGYAPGM QSRDFISVED VVKVNLHFFD HPDKSGIFNL 

       250        260        270        280        290        300 
GTGRAQPFND IAATVVNTLR EAEGKPRLSL DELVQEGLLE YVKFPDALRG KYQCFTQSDV 

       310        320        330 
SKLRSAGYSE PFLSVEEGVA RYCRWLIERA G

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References

[1]"Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia eutropha H16."
Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I., Gottschalk G., Steinbuechel A., Friedrich B., Bowien B.
Nat. Biotechnol. 24:1257-1262(2006) [PubMed: 16964242] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

AM260479 Genomic DNA. Translation: CAJ91951.1.
RefSeqYP_725319.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID4250456.
GenomeReviewsGene locus H16_A0804 in contig AM260479_GR.
KEGGreh:H16_A0804.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ0KDH0.
OMAQ0KDH0. FGPNEYH.

Family and domain databases

HAMAPMF_01601.
[Tree]
InterProIPR001509. Epimerase_deHydtase.
IPR011912. Heptose_epim.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHERPTHR10366:SF29. Heptose_epim. 1 hit.
PfamPF01370. Epimerase. 1 hit.
[Graphical view]
TIGRFAMsTIGR02197. heptose_epim. 1 hit.
ProtoNetSearch...

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Entry information

Entry nameHLDD_RALEH
AccessionPrimary (citable) accession number: Q0KDH0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 3, 2006
Last modified: June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents