Reviewed, UniProtKB/Swiss-Prot Q0KCY0 (F16A1_RALEH)

Names and origin

Protein names

Recommended name:
    Fructose-1,6-bisphosphatase class 1 1
      Short name=FBPase class 1 1
    EC=3.1.3.11
Alternative name(s):
    D-fructose-1,6-bisphosphate 1-phosphohydrolase class 1 1

Gene names

Name:	fbp1
Ordered Locus Names:	H16_A0999

Organism

Ralstonia eutropha (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)) [Complete proteome] [HAMAP]

Taxonomic identifier

381666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate. HAMAP MF_01855
Cofactor	Binds 2 magnesium ions per subunit By similarity. HAMAP MF_01855
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_01855
Subunit structure	Homotetramer By similarity. HAMAP MF_01855
Subcellular location	Cytoplasm Potential HAMAP MF_01855.
Sequence similarities	Belongs to the FBPase class 1 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	fructose 1,6-bisphosphate 1-phosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	Fructose-1,6-bisphosphatase class 1 1 HAMAP MF_01855		PRO_0000364660
Regions
	Region	116 – 119	4	Substrate binding By similarity
Sites
	Metal binding	91	1	Magnesium 1 By similarity
	Metal binding	113	1	Magnesium 1 By similarity
	Metal binding	113	1	Magnesium 2 By similarity
	Metal binding	115	1	Magnesium 1; via carbonyl oxygen By similarity
	Metal binding	116	1	Magnesium 2 By similarity
	Metal binding	280	1	Magnesium 2 By similarity
	Binding site	208	1	Substrate By similarity
	Binding site	274	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0KCY0-1 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: 68F8D352B016E09C	FASTA	338	37,505
	10 20 30 40 50 60 MTRISLTRYL VEEQRKHNTI QPELRLLIEV VARACKAISN AVSKGALAGV LGSAGTGNVQ 70 80 90 100 110 120 GETQQKLDVI ANEVLLDANE WGGHLAAMAS EEMESFYEIP NRYPKGEYLL MFDPLDGSSN 130 140 150 160 170 180 IDVNVSIGTI FSVLHMPKPG QTVTEADFMQ PGTHQVAAGY AVYGPQTTLV LTVGNGVHMF 190 200 210 220 230 240 TLDREAGSFV LTHSNVTIPD DTKEFAINMS NMRHWAPPVR RYIDECLAGE EGPRGKNFNM 250 260 270 280 290 300 RWVASMVADV HRILTRGGVF MYPWDKREPE KPGKLRLMYE ANPMAMLVEQ AGGAATNGHQ 310 320 330 RIMDVQPEKL HQRVSVILGS KNEVERVTRY HLEAEDKA

References

[1]

"Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia eutropha H16." Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I., Gottschalk G., Steinbuechel A., Friedrich B., Bowien B. Nat. Biotechnol. 24:1257-1262(2006) [PubMed: 16964242] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM260479 Genomic DNA. Translation: CAJ92141.1.
RefSeq	YP_725509.1.
3D structure databases
SMR	Q0KCY0. Positions 4-336.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0KCY0.
Genome annotation databases
GeneID	4246989.
GenomeReviews	Gene locus H16_A0999 in contig AM260479_GR.
KEGG	reh:H16_A0999.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0158.
HOGENOM	HBG731261.
OMA	HWEAPVQ.
PhylomeDB	Q0KCY0.
Family and domain databases
HAMAP	MF_01855. FBPase_class1. [Tree]
InterPro	IPR000146. Fructose_bisphosphatase. [Graphical view]
PANTHER	PTHR11556. In_FB_phphtase. 1 hit.
Pfam	PF00316. FBPase. 1 hit. [Graphical view]
PRINTS	PR00115. F16BPHPHTASE.
PROSITE	PS00124. FBPASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

F16A1_RALEH

Accession

Primary (citable) accession number: Q0KCY0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	October 3, 2006
Last modified:	January 19, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)