Q0KCW5 (ADE_CUPNH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenine deaminase
Short name=ADE
EC=3.5.4.2

Alternative name(s):
Adenine aminohydrolase
Short name=AAH

Gene names

Ordered Locus Names:

H16_A1014

Organism

Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha) [Complete proteome] [HAMAP]

Taxonomic identifier

381666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the hydrolytic deamination of adenine to hypoxanthine. Plays an important role in the purine salvage pathway and in nitrogen catabolism By similarity. HAMAP MF_01962
Catalytic activity	Adenine + H2O = hypoxanthine + NH3. HAMAP MF_01962
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_01962
Sequence similarities	Belongs to the adenosine and AMP deaminases family. Adenine deaminase type 2 subfamily.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	adenine deaminase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	Adenine deaminase HAMAP MF_01962		PRO_1000017687
Sites
	Active site	203	1	Proton donor By similarity
	Metal binding	20	1	Zinc; catalytic By similarity
	Metal binding	22	1	Zinc; catalytic By similarity
	Metal binding	200	1	Zinc; catalytic By similarity
	Metal binding	281	1	Zinc; catalytic By similarity
	Binding site	282	1	Substrate By similarity
	Site	224	1	Important for catalytic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0KCW5 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: ADB22FC13F99CCFA	FASTA	351	38,865
	10 20 30 40 50 60 MTIDAALAEQ IRRTPKAELH VHIEGTLEPE LIFRLAQRNQ VALPYPSVEA LRAAYAFTDL 70 80 90 100 110 120 QSFLDIYYAG ASVLLTEEDF FDMTMDYVKR AVADNVRHAE IFFDPQTHTA RGVPIGVVID 130 140 150 160 170 180 GIADALAQAR TEYDFSSSLI LCFLRHLPEE DAFATLEAAL PYRDRFVGVG LDSSERGNPP 190 200 210 220 230 240 EKFARVFARA RELGLHLVAH AGEEGPAQYV TDALDILKAQ RIDHGVRAID DPALVERLAR 250 260 270 280 290 300 ERVALTVCPL SNVKLKVYPD LRDHPLKRML DAGVVITLHS DDPAYFGGYM NANWEATFEA 310 320 330 340 350 LPLDAADAHK LARNSFEAAF LPAMQKAEFL AEVDHFWSSP PKSPPATAPA A

References

[1]

"Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia eutropha H16." Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I., Gottschalk G., Steinbuechel A., Friedrich B., Bowien B. Nat. Biotechnol. 24:1257-1262(2006) [PubMed: 16964242] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM260479 Genomic DNA. Translation: CAJ92156.1.
RefSeq	YP_725524.1. NC_008313.1.
3D structure databases
ProteinModelPortal	Q0KCW5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0KCW5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4247149.
GenomeReviews	Gene locus H16_A1014 in contig AM260479_GR.
KEGG	reh:H16_A1014.
PATRIC	35231459. VBIRalEut6770_1393.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1816.
HOGENOM	HBG630382.
OMA	SYIYEAL.
PhylomeDB	Q0KCW5.
ProtClustDB	PRK09358.
Enzyme and pathway databases
BioCyc	REUT381666:H16_A1014-MONOMER.
Family and domain databases
HAMAP	MF_01962. Adenine_deaminase. [Tree]
InterPro	IPR001365. A/AMP_deaminase_dom. IPR006330. A_deaminase. [Graphical view]
KO	K01488.
Pfam	PF00962. A_deaminase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01430. Aden_deam. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ADE_CUPNH

Accession

Primary (citable) accession number: Q0KCW5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 3, 2006
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program