Q0KCE4 (KDSA_CUPNH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-dehydro-3-deoxyphosphooctonate aldolase
EC=2.5.1.55

Alternative name(s):
3-deoxy-D-manno-octulosonic acid 8-phosphate synthase
KDO-8-phosphate synthase
Short name=KDO 8-P synthase
Short name=KDOPS
Phospho-2-dehydro-3-deoxyoctonate aldolase

Gene names

Name:	kdsA
Ordered Locus Names:	H16_A1186

Organism

Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha) [Complete proteome] [HAMAP]

Taxonomic identifier

381666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + D-arabinose 5-phosphate + H2O = 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate. HAMAP MF_00056
Pathway	Carbohydrate biosynthesis; 3-deoxy-D-manno-octulosonate biosynthesis; 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate: step 2/3. HAMAP MF_00056 Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. HAMAP MF_00056
Subcellular location	Cytoplasm By similarity HAMAP MF_00056.
Sequence similarities	Belongs to the KdsA family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-deoxy-8-phosphooctulonate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 289	289	2-dehydro-3-deoxyphosphooctonate aldolase HAMAP MF_00056		PRO_0000304477

Sequences

	Sequence		Length	Mass (Da)
	Q0KCE4 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: 99325FFFE9A89B5D	FASTA	289	30,987
	10 20 30 40 50 60 MKLCGFEAGL DKPFFLIAGP CVIESEQMAL DTAGELKAIT AELGIPFIYK SSFDKANRSS 70 80 90 100 110 120 GKSFRGLGME KGLEILATVK REIGVPVLTD IHEIDEIKPV AAVVDVLQTP AFLCRQTDFI 130 140 150 160 170 180 RACAQSGKPV NIKKGQFLAP HDMKNVIDKA RDAAREAGLP DDVFMACERG VSFGYNNLVS 190 200 210 220 230 240 DMRSLAIMRE TGAPVVFDAT HSVQLPGGQG TSSGGQREFV PVLSRAAVAT GVAGLFMETH 250 260 270 280 PDPSKAMSDG PNAVPLSRMK ELLAVLKELD TLVKRAGFLE DNFGWPACA

References

[1]

"Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia eutropha H16." Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I., Gottschalk G., Steinbuechel A., Friedrich B., Bowien B. Nat. Biotechnol. 24:1257-1262(2006) [PubMed: 16964242] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM260479 Genomic DNA. Translation: CAJ92327.1.
RefSeq	YP_725695.1. NC_008313.1.
3D structure databases
ProteinModelPortal	Q0KCE4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0KCE4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4250345.
GenomeReviews	Gene locus H16_A1186 in contig AM260479_GR.
KEGG	reh:H16_A1186.
PATRIC	35231823. VBIRalEut6770_1574.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2877.
HOGENOM	HBG575990.
OMA	VFMACER.
PhylomeDB	Q0KCE4.
ProtClustDB	PRK05198.
Enzyme and pathway databases
BioCyc	REUT381666:H16_A1186-MONOMER.
Family and domain databases
HAMAP	MF_00056. KDO8P_synth. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR006218. DAHP1/KDSA. IPR006269. KDO8P_synthase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01627.
PANTHER	PTHR21057:SF2. KDO8P_synthase. 1 hit.
Pfam	PF00793. DAHP_synth_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01362. KDO8P_synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSA_CUPNH

Accession

Primary (citable) accession number: Q0KCE4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	October 3, 2006
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program