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Q0KCA9

- SYC_CUPNH

UniProt

Q0KCA9 - SYC_CUPNH

Protein

Cysteine--tRNA ligase

Gene

cysS

Organism
Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (03 Oct 2006)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi30 – 301ZincUniRule annotation
    Metal bindingi214 – 2141ZincUniRule annotation
    Metal bindingi239 – 2391ZincUniRule annotation
    Metal bindingi243 – 2431ZincUniRule annotation
    Binding sitei274 – 2741ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. cysteine-tRNA ligase activity Source: UniProtKB-HAMAP
    3. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cysteinyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciCNEC381666:GJUJ-1218-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cysteine--tRNA ligaseUniRule annotation (EC:6.1.1.16UniRule annotation)
    Alternative name(s):
    Cysteinyl-tRNA synthetaseUniRule annotation
    Short name:
    CysRSUniRule annotation
    Gene namesi
    Name:cysSUniRule annotation
    Ordered Locus Names:H16_A1221
    OrganismiCupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
    Taxonomic identifieri381666 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
    ProteomesiUP000008210: Chromosome 1

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 462462Cysteine--tRNA ligasePRO_1000006602Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi381666.H16_A1221.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0KCA9.
    SMRiQ0KCA9. Positions 3-402.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi32 – 4211"HIGH" regionAdd
    BLAST
    Motifi271 – 2755"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0215.
    HOGENOMiHOG000245250.
    KOiK01883.
    OMAiHIGNIRA.
    OrthoDBiEOG6RVFXC.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    HAMAPiMF_00041. Cys_tRNA_synth.
    InterProiIPR015803. Cys-tRNA-ligase.
    IPR015273. Cys-tRNA-synt_Ia_DALR.
    IPR024909. Cys-tRNA/MSH_ligase.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    [Graphical view]
    PANTHERiPTHR10890. PTHR10890. 1 hit.
    PfamiPF09190. DALR_2. 1 hit.
    PF01406. tRNA-synt_1e. 1 hit.
    [Graphical view]
    PRINTSiPR00983. TRNASYNTHCYS.
    SMARTiSM00840. DALR_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF47323. SSF47323. 1 hit.
    TIGRFAMsiTIGR00435. cysS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q0KCA9-1 [UniParc]FASTAAdd to Basket

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    MQLLNIYNTL AREKQPFVPI EPGKVRMYVC GMTVYDYCHV GHARVMVVFD    50
    MVHRWLRAAG YEVTYVQNIT DIDDKIIRRA AENGETIGEL TTRFIQYMHE 100
    DAAALGVIRP DHEPRATDYV PQMLDMIGKL EAKGLAYQAS DGDVNYSVRK 150
    FAGYGKLSGK SLEDLRAGER VSANDAKQDP LDFVLWKSAK ASEPPESKWD 200
    SKWGAGRPGW HIECSAMSCT LLGEHFDIHG GGADLQFPHH ENEIAQSEGA 250
    SGKPFVNMWM HNGFVRINDE KMSKSLGNFF TIREVLKSYD AEVVRFFILR 300
    AHYRSPLNYS DAHLDDARHA LTRLYTALKD SQPGGCAVDW DEPHARRFAE 350
    AMGDDFNTPI AMSVLFDLAS EINRTGSTAA ARQLKGLAGT LGLLERDPHT 400
    FLQGGRSDDG ISPEQIETLI AARKTAKVER NFAEADRIRA QLLEAGIVLE 450
    DKPGGATEWR RA 462
    Length:462
    Mass (Da):51,650
    Last modified:October 3, 2006 - v1
    Checksum:i37AAAAE74DD126A2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM260479 Genomic DNA. Translation: CAJ92362.1.
    RefSeqiYP_725730.1. NC_008313.1.

    Genome annotation databases

    EnsemblBacteriaiCAJ92362; CAJ92362; H16_A1221.
    GeneIDi4247019.
    KEGGireh:H16_A1221.
    PATRICi35231895. VBIRalEut6770_1610.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM260479 Genomic DNA. Translation: CAJ92362.1 .
    RefSeqi YP_725730.1. NC_008313.1.

    3D structure databases

    ProteinModelPortali Q0KCA9.
    SMRi Q0KCA9. Positions 3-402.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 381666.H16_A1221.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAJ92362 ; CAJ92362 ; H16_A1221 .
    GeneIDi 4247019.
    KEGGi reh:H16_A1221.
    PATRICi 35231895. VBIRalEut6770_1610.

    Phylogenomic databases

    eggNOGi COG0215.
    HOGENOMi HOG000245250.
    KOi K01883.
    OMAi HIGNIRA.
    OrthoDBi EOG6RVFXC.

    Enzyme and pathway databases

    BioCyci CNEC381666:GJUJ-1218-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    HAMAPi MF_00041. Cys_tRNA_synth.
    InterProi IPR015803. Cys-tRNA-ligase.
    IPR015273. Cys-tRNA-synt_Ia_DALR.
    IPR024909. Cys-tRNA/MSH_ligase.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    [Graphical view ]
    PANTHERi PTHR10890. PTHR10890. 1 hit.
    Pfami PF09190. DALR_2. 1 hit.
    PF01406. tRNA-synt_1e. 1 hit.
    [Graphical view ]
    PRINTSi PR00983. TRNASYNTHCYS.
    SMARTi SM00840. DALR_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47323. SSF47323. 1 hit.
    TIGRFAMsi TIGR00435. cysS. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

    Entry informationi

    Entry nameiSYC_CUPNH
    AccessioniPrimary (citable) accession number: Q0KCA9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: October 3, 2006
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3