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Q0KCA9

- SYC_CUPNH

UniProt

Q0KCA9 - SYC_CUPNH

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Protein

Cysteine--tRNA ligase

Gene

cysS

Organism
Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi30 – 301ZincUniRule annotation
Metal bindingi214 – 2141ZincUniRule annotation
Metal bindingi239 – 2391ZincUniRule annotation
Metal bindingi243 – 2431ZincUniRule annotation
Binding sitei274 – 2741ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. cysteine-tRNA ligase activity Source: UniProtKB-HAMAP
  3. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. cysteinyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCNEC381666:GJUJ-1218-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine--tRNA ligaseUniRule annotation (EC:6.1.1.16UniRule annotation)
Alternative name(s):
Cysteinyl-tRNA synthetaseUniRule annotation
Short name:
CysRSUniRule annotation
Gene namesi
Name:cysSUniRule annotation
Ordered Locus Names:H16_A1221
OrganismiCupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
Taxonomic identifieri381666 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
ProteomesiUP000008210: Chromosome 1

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 462462Cysteine--tRNA ligasePRO_1000006602Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi381666.H16_A1221.

Structurei

3D structure databases

ProteinModelPortaliQ0KCA9.
SMRiQ0KCA9. Positions 3-402.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi32 – 4211"HIGH" regionAdd
BLAST
Motifi271 – 2755"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0215.
HOGENOMiHOG000245250.
KOiK01883.
OMAiHIGNIRA.
OrthoDBiEOG6RVFXC.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00041. Cys_tRNA_synth.
InterProiIPR015803. Cys-tRNA-ligase.
IPR015273. Cys-tRNA-synt_Ia_DALR.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF09190. DALR_2. 1 hit.
PF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
SMARTiSM00840. DALR_2. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00435. cysS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0KCA9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQLLNIYNTL AREKQPFVPI EPGKVRMYVC GMTVYDYCHV GHARVMVVFD
60 70 80 90 100
MVHRWLRAAG YEVTYVQNIT DIDDKIIRRA AENGETIGEL TTRFIQYMHE
110 120 130 140 150
DAAALGVIRP DHEPRATDYV PQMLDMIGKL EAKGLAYQAS DGDVNYSVRK
160 170 180 190 200
FAGYGKLSGK SLEDLRAGER VSANDAKQDP LDFVLWKSAK ASEPPESKWD
210 220 230 240 250
SKWGAGRPGW HIECSAMSCT LLGEHFDIHG GGADLQFPHH ENEIAQSEGA
260 270 280 290 300
SGKPFVNMWM HNGFVRINDE KMSKSLGNFF TIREVLKSYD AEVVRFFILR
310 320 330 340 350
AHYRSPLNYS DAHLDDARHA LTRLYTALKD SQPGGCAVDW DEPHARRFAE
360 370 380 390 400
AMGDDFNTPI AMSVLFDLAS EINRTGSTAA ARQLKGLAGT LGLLERDPHT
410 420 430 440 450
FLQGGRSDDG ISPEQIETLI AARKTAKVER NFAEADRIRA QLLEAGIVLE
460
DKPGGATEWR RA
Length:462
Mass (Da):51,650
Last modified:October 3, 2006 - v1
Checksum:i37AAAAE74DD126A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM260479 Genomic DNA. Translation: CAJ92362.1.
RefSeqiYP_725730.1. NC_008313.1.

Genome annotation databases

EnsemblBacteriaiCAJ92362; CAJ92362; H16_A1221.
GeneIDi4247019.
KEGGireh:H16_A1221.
PATRICi35231895. VBIRalEut6770_1610.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM260479 Genomic DNA. Translation: CAJ92362.1 .
RefSeqi YP_725730.1. NC_008313.1.

3D structure databases

ProteinModelPortali Q0KCA9.
SMRi Q0KCA9. Positions 3-402.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 381666.H16_A1221.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAJ92362 ; CAJ92362 ; H16_A1221 .
GeneIDi 4247019.
KEGGi reh:H16_A1221.
PATRICi 35231895. VBIRalEut6770_1610.

Phylogenomic databases

eggNOGi COG0215.
HOGENOMi HOG000245250.
KOi K01883.
OMAi HIGNIRA.
OrthoDBi EOG6RVFXC.

Enzyme and pathway databases

BioCyci CNEC381666:GJUJ-1218-MONOMER.

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
HAMAPi MF_00041. Cys_tRNA_synth.
InterProi IPR015803. Cys-tRNA-ligase.
IPR015273. Cys-tRNA-synt_Ia_DALR.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view ]
PANTHERi PTHR10890. PTHR10890. 1 hit.
Pfami PF09190. DALR_2. 1 hit.
PF01406. tRNA-synt_1e. 1 hit.
[Graphical view ]
PRINTSi PR00983. TRNASYNTHCYS.
SMARTi SM00840. DALR_2. 1 hit.
[Graphical view ]
SUPFAMi SSF47323. SSF47323. 1 hit.
TIGRFAMsi TIGR00435. cysS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

Entry informationi

Entry nameiSYC_CUPNH
AccessioniPrimary (citable) accession number: Q0KCA9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 3, 2006
Last modified: November 26, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3