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Q0KCA9 (SYC_CUPNH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cysteine--tRNA ligase

EC=6.1.1.16
Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS
Gene names
Name:cysS
Ordered Locus Names:H16_A1221
OrganismCupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha) [Complete proteome] [HAMAP]
Taxonomic identifier381666 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus

Protein attributes

Sequence length462 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP-Rule MF_00041

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00041

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00041

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00041.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcysteinyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

cysteine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 462462Cysteine--tRNA ligase HAMAP-Rule MF_00041
PRO_1000006602

Regions

Motif32 – 4211"HIGH" region HAMAP-Rule MF_00041
Motif271 – 2755"KMSKS" region HAMAP-Rule MF_00041

Sites

Metal binding301Zinc By similarity
Metal binding2141Zinc By similarity
Metal binding2391Zinc By similarity
Metal binding2431Zinc By similarity
Binding site2741ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0KCA9 [UniParc].

Last modified October 3, 2006. Version 1.
Checksum: 37AAAAE74DD126A2

FASTA46251,650
        10         20         30         40         50         60 
MQLLNIYNTL AREKQPFVPI EPGKVRMYVC GMTVYDYCHV GHARVMVVFD MVHRWLRAAG 

        70         80         90        100        110        120 
YEVTYVQNIT DIDDKIIRRA AENGETIGEL TTRFIQYMHE DAAALGVIRP DHEPRATDYV 

       130        140        150        160        170        180 
PQMLDMIGKL EAKGLAYQAS DGDVNYSVRK FAGYGKLSGK SLEDLRAGER VSANDAKQDP 

       190        200        210        220        230        240 
LDFVLWKSAK ASEPPESKWD SKWGAGRPGW HIECSAMSCT LLGEHFDIHG GGADLQFPHH 

       250        260        270        280        290        300 
ENEIAQSEGA SGKPFVNMWM HNGFVRINDE KMSKSLGNFF TIREVLKSYD AEVVRFFILR 

       310        320        330        340        350        360 
AHYRSPLNYS DAHLDDARHA LTRLYTALKD SQPGGCAVDW DEPHARRFAE AMGDDFNTPI 

       370        380        390        400        410        420 
AMSVLFDLAS EINRTGSTAA ARQLKGLAGT LGLLERDPHT FLQGGRSDDG ISPEQIETLI 

       430        440        450        460 
AARKTAKVER NFAEADRIRA QLLEAGIVLE DKPGGATEWR RA 

« Hide

References

[1]"Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia eutropha H16."
Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I., Gottschalk G., Steinbuechel A., Friedrich B., Bowien B.
Nat. Biotechnol. 24:1257-1262(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM260479 Genomic DNA. Translation: CAJ92362.1.
RefSeqYP_725730.1. NC_008313.1.

3D structure databases

ProteinModelPortalQ0KCA9.
SMRQ0KCA9. Positions 3-402.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING381666.H16_A1221.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAJ92362; CAJ92362; H16_A1221.
GeneID4247019.
KEGGreh:H16_A1221.
PATRIC35231895. VBIRalEut6770_1610.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0215.
HOGENOMHOG000245250.
KOK01883.
OMAHIGNIRA.
OrthoDBEOG6RVFXC.

Enzyme and pathway databases

BioCycCNEC381666:GJUJ-1218-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00041. Cys_tRNA_synth.
InterProIPR015803. Cys-tRNA-ligase.
IPR015273. Cys-tRNA-synt_Ia_DALR.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PANTHERPTHR10890. PTHR10890. 1 hit.
PfamPF09190. DALR_2. 1 hit.
PF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSPR00983. TRNASYNTHCYS.
SMARTSM00840. DALR_2. 1 hit.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
TIGRFAMsTIGR00435. cysS. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYC_CUPNH
AccessionPrimary (citable) accession number: Q0KCA9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 3, 2006
Last modified: May 14, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries