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Protein

Cysteine--tRNA ligase

Gene

cysS

Organism
Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi30ZincUniRule annotation1
Metal bindingi214ZincUniRule annotation1
Metal bindingi239ZincUniRule annotation1
Metal bindingi243ZincUniRule annotation1
Binding sitei274ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine--tRNA ligaseUniRule annotation (EC:6.1.1.16UniRule annotation)
Alternative name(s):
Cysteinyl-tRNA synthetaseUniRule annotation
Short name:
CysRSUniRule annotation
Gene namesi
Name:cysSUniRule annotation
Ordered Locus Names:H16_A1221
OrganismiCupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
Taxonomic identifieri381666 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
Proteomesi
  • UP000008210 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000066021 – 462Cysteine--tRNA ligaseAdd BLAST462

Proteomic databases

PRIDEiQ0KCA9

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi381666.H16_A1221

Structurei

3D structure databases

ProteinModelPortaliQ0KCA9
SMRiQ0KCA9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi32 – 42"HIGH" regionAdd BLAST11
Motifi271 – 275"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C8N Bacteria
COG0215 LUCA
HOGENOMiHOG000245250
KOiK01883
OMAiHECEIAQ

Family and domain databases

CDDicd00672 CysRS_core, 1 hit
Gene3Di3.40.50.620, 1 hit
HAMAPiMF_00041 Cys_tRNA_synth, 1 hit
InterProiView protein in InterPro
IPR015803 Cys-tRNA-ligase
IPR015273 Cys-tRNA-synt_Ia_DALR
IPR024909 Cys-tRNA/MSH_ligase
IPR014729 Rossmann-like_a/b/a_fold
IPR032678 tRNA-synt_1_cat_dom
IPR009080 tRNAsynth_Ia_anticodon-bd
PANTHERiPTHR10890 PTHR10890, 1 hit
PfamiView protein in Pfam
PF09190 DALR_2, 1 hit
PF01406 tRNA-synt_1e, 1 hit
PRINTSiPR00983 TRNASYNTHCYS
SMARTiView protein in SMART
SM00840 DALR_2, 1 hit
SUPFAMiSSF47323 SSF47323, 1 hit
TIGRFAMsiTIGR00435 cysS, 1 hit

Sequencei

Sequence statusi: Complete.

Q0KCA9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQLLNIYNTL AREKQPFVPI EPGKVRMYVC GMTVYDYCHV GHARVMVVFD
60 70 80 90 100
MVHRWLRAAG YEVTYVQNIT DIDDKIIRRA AENGETIGEL TTRFIQYMHE
110 120 130 140 150
DAAALGVIRP DHEPRATDYV PQMLDMIGKL EAKGLAYQAS DGDVNYSVRK
160 170 180 190 200
FAGYGKLSGK SLEDLRAGER VSANDAKQDP LDFVLWKSAK ASEPPESKWD
210 220 230 240 250
SKWGAGRPGW HIECSAMSCT LLGEHFDIHG GGADLQFPHH ENEIAQSEGA
260 270 280 290 300
SGKPFVNMWM HNGFVRINDE KMSKSLGNFF TIREVLKSYD AEVVRFFILR
310 320 330 340 350
AHYRSPLNYS DAHLDDARHA LTRLYTALKD SQPGGCAVDW DEPHARRFAE
360 370 380 390 400
AMGDDFNTPI AMSVLFDLAS EINRTGSTAA ARQLKGLAGT LGLLERDPHT
410 420 430 440 450
FLQGGRSDDG ISPEQIETLI AARKTAKVER NFAEADRIRA QLLEAGIVLE
460
DKPGGATEWR RA
Length:462
Mass (Da):51,650
Last modified:October 3, 2006 - v1
Checksum:i37AAAAE74DD126A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM260479 Genomic DNA Translation: CAJ92362.1
RefSeqiWP_011614939.1, NC_008313.1

Genome annotation databases

EnsemblBacteriaiCAJ92362; CAJ92362; H16_A1221
KEGGireh:H16_A1221
PATRICifig|381666.6.peg.1610

Similar proteinsi

Entry informationi

Entry nameiSYC_CUPNH
AccessioniPrimary (citable) accession number: Q0KCA9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 3, 2006
Last modified: May 23, 2018
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health