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Q0K9B9 (IF2_CUPNH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Translation initiation factor IF-2
Gene names
Name:infB
Ordered Locus Names:H16_A2306
OrganismCupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha) [Complete proteome] [HAMAP]
Taxonomic identifier381666 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus

Protein attributes

Sequence length962 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex By similarity. HAMAP-Rule MF_00100

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00100.

Sequence similarities

Belongs to the IF-2 family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandGTP-binding
Nucleotide-binding
   Molecular functionInitiation factor
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTPase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

translation initiation factor activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 962962Translation initiation factor IF-2 HAMAP-Rule MF_00100
PRO_1000008310

Regions

Nucleotide binding471 – 4788GTP By similarity
Nucleotide binding517 – 5215GTP By similarity
Nucleotide binding571 – 5744GTP By similarity
Region465 – 613149G-domain HAMAP-Rule MF_00100

Sequences

Sequence LengthMass (Da)Tools
Q0K9B9 [UniParc].

Last modified October 3, 2006. Version 1.
Checksum: AB8F163CF73F5741

FASTA962103,710
        10         20         30         40         50         60 
MASTTVAQLA AELSRSAAAL LEQLQAAGVG KATPEDIITE SDKTRLLDYL KRSHGQADDS 

        70         80         90        100        110        120 
SRKKITLTKR ETSEIRQSDG TGKTRTVQVE VRKKRVLIKR DEAAPDAQAD AVEAQAPVVD 

       130        140        150        160        170        180 
AVEEARRDEE ERQQAELLAR QEAEAKAARE AAEREEAERR ARQEALEAEQ RRQAELAARK 

       190        200        210        220        230        240 
AEEEAAASRA VTEANEDSSR KKAEDEKARV TAERAEAQKA ADEAKAAADK ARAEQEVAAR 

       250        260        270        280        290        300 
KRREAAEAEA RAIQQMLNAP PRVLKAPSER KAEEKKAEQT GTLHKPVKPA GATTEAKKDE 

       310        320        330        340        350        360 
KKPATTTTTT ATADKKGKVV KAGWQDDSSR KKGSGLKTRG DTSGGVGGWR GGPRGRGGRQ 

       370        380        390        400        410        420 
QQHDDSRSSF QAPTEPVVRE VHVPETVSVA DLAHKMAVKA SEVIKQMMKL GQMVTINQVL 

       430        440        450        460        470        480 
DQETAMIVVE EMGHKAYAAK LDDPEALLVV GGEEHTDAEL LPRPPVVTVM GHVDHGKTSL 

       490        500        510        520        530        540 
LDYIRRTKVA AGEAGGITQH IGAYHVETDR GVITFLDTPG HEAFTAMRAR GAKATDIVIL 

       550        560        570        580        590        600 
VVAADDGVMP QTKEAIAHAK AAGVPIVVAI NKIDKPDANP DRVKQELVAE QVVPEEYGGD 

       610        620        630        640        650        660 
SPFVPVSAKM GTGVEDLLEQ VLLQAEVLEL TAPVDAPAKG LVVEAQLDKG KGPIATILVS 

       670        680        690        700        710        720 
SGTLKRGDVV LAGSAYGRVR AMLDENGKPT KEAGPSIPVE IQGLSEVPAA GEEVLVLPDE 

       730        740        750        760        770        780 
RKAREIALFR QGKFRDVKLA KQQAAKLENM LEQMAEGEVQ TLPLIVKADV QGSQEALVQS 

       790        800        810        820        830        840 
LQKLSTAEVR VQIVHGGVGG ISESDVNLAT ASKAVIIGFN VRADAGARKL AEHNGIDIRY 

       850        860        870        880        890        900 
YNIIYDAVDE IKAAMSGMLA PEKRETTIGQ VEVRQVFRVP KIGAVAGCMV TDGLVKRNSL 

       910        920        930        940        950        960 
VRVLRNNVVI HDGELDSLKR FKDDVKEVKQ GFECGLSIKN FNDVQEGDQL EVYEITEVAR 


TL 

« Hide

References

[1]"Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia eutropha H16."
Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I., Gottschalk G., Steinbuechel A., Friedrich B., Bowien B.
Nat. Biotechnol. 24:1257-1262(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM260479 Genomic DNA. Translation: CAJ93402.1.
RefSeqYP_726770.1. NC_008313.1.

3D structure databases

ProteinModelPortalQ0K9B9.
SMRQ0K9B9. Positions 865-960.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING381666.H16_A2306.

Proteomic databases

PRIDEQ0K9B9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAJ93402; CAJ93402; H16_A2306.
GeneID4250192.
KEGGreh:H16_A2306.
PATRIC35234125. VBIRalEut6770_2709.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0532.
HOGENOMHOG000076907.
KOK02519.
OMAMMKLGQM.
OrthoDBEOG67HJSV.
ProtClustDBPRK05306.

Enzyme and pathway databases

BioCycCNEC381666:GJUJ-2274-MONOMER.

Family and domain databases

Gene3D3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPMF_00100_B. IF_2_B.
InterProIPR009061. DNA-bd_dom_put.
IPR000795. EF_GTP-bd_dom.
IPR013575. IF2_assoc_dom_bac.
IPR006847. IF2_N.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000178. TF_IF2_bacterial-like.
IPR015760. TIF_IF2.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PANTHERPTHR23115:SF41. PTHR23115:SF41. 1 hit.
PfamPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 2 hits.
PF11987. IF-2. 1 hit.
PF08364. IF2_assoc. 1 hit.
PF04760. IF2_N. 2 hits.
[Graphical view]
SUPFAMSSF46955. SSF46955. 1 hit.
SSF50447. SSF50447. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00487. IF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEPS01176. IF2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIF2_CUPNH
AccessionPrimary (citable) accession number: Q0K9B9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 3, 2006
Last modified: April 16, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Translation initiation factors

List of translation initiation factor entries