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Q0K889

- TYSY_CUPNH

UniProt

Q0K889 - TYSY_CUPNH

Protein

Thymidylate synthase

Gene

thyA

Organism
Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 2 (26 Feb 2008)
      Previous versions | rss
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    Functioni

    Provides the sole de novo source of dTMP for DNA biosynthesis.UniRule annotation

    Catalytic activityi

    5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei146 – 1461UniRule annotation

    GO - Molecular functioni

    1. thymidylate synthase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. dTMP biosynthetic process Source: UniProtKB-HAMAP
    2. dTTP biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Nucleotide biosynthesis

    Enzyme and pathway databases

    BioCyciCNEC381666:GJUJ-2668-MONOMER.
    UniPathwayiUPA00575.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
    Short name:
    TSUniRule annotation
    Short name:
    TSaseUniRule annotation
    Gene namesi
    Name:thyAUniRule annotation
    Ordered Locus Names:H16_A2703
    OrganismiCupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
    Taxonomic identifieri381666 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
    ProteomesiUP000008210: Chromosome 1

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 264264Thymidylate synthasePRO_0000321477Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi381666.H16_A2703.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0K889.
    SMRiQ0K889. Positions 1-264.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0207.
    HOGENOMiHOG000257899.
    KOiK00560.
    OMAiFEYEDIA.
    OrthoDBiEOG6K6V53.

    Family and domain databases

    Gene3Di3.30.572.10. 1 hit.
    HAMAPiMF_00008. Thymidy_synth_bact.
    InterProiIPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view]
    PfamiPF00303. Thymidylat_synt. 1 hit.
    [Graphical view]
    PRINTSiPR00108. THYMDSNTHASE.
    SUPFAMiSSF55831. SSF55831. 1 hit.
    TIGRFAMsiTIGR03284. thym_sym. 2 hits.
    PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q0K889-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKQYLDFMRH VYEHGTDKAD RTGTGTRSVF GYQMRFDLRA GFPVVTTKKL    50
    HLKSIIYELL WFLQGSTNVR WLQEHGVTIW DEWADENGEL GPVYGSQWRS 100
    WPTPDGRHID QITDLVAQIR ANPDSRRLIV SAWNVADIPR MKLPPCHAFF 150
    QFYVADGRLS CQLYQRSADI FLGVPFNIAS YALLTHMMAQ QTGLEVGDFV 200
    WTGGDCHLYN NHFEQVQTQL AREPLALPQL KILRKPDSIF DYRYEDFELA 250
    GYQSHAAIKA PVAV 264
    Length:264
    Mass (Da):30,429
    Last modified:February 26, 2008 - v2
    Checksum:i052CAFE7F65F631C
    GO

    Sequence cautioni

    The sequence CAJ93782.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM260479 Genomic DNA. Translation: CAJ93782.1. Different initiation.
    RefSeqiWP_011615791.1. NC_008313.1.
    YP_727150.1. NC_008313.1.

    Genome annotation databases

    EnsemblBacteriaiCAJ93782; CAJ93782; H16_A2703.
    GeneIDi4249109.
    KEGGireh:H16_A2703.
    PATRICi35234919. VBIRalEut6770_3092.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM260479 Genomic DNA. Translation: CAJ93782.1 . Different initiation.
    RefSeqi WP_011615791.1. NC_008313.1.
    YP_727150.1. NC_008313.1.

    3D structure databases

    ProteinModelPortali Q0K889.
    SMRi Q0K889. Positions 1-264.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 381666.H16_A2703.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAJ93782 ; CAJ93782 ; H16_A2703 .
    GeneIDi 4249109.
    KEGGi reh:H16_A2703.
    PATRICi 35234919. VBIRalEut6770_3092.

    Phylogenomic databases

    eggNOGi COG0207.
    HOGENOMi HOG000257899.
    KOi K00560.
    OMAi FEYEDIA.
    OrthoDBi EOG6K6V53.

    Enzyme and pathway databases

    UniPathwayi UPA00575 .
    BioCyci CNEC381666:GJUJ-2668-MONOMER.

    Family and domain databases

    Gene3Di 3.30.572.10. 1 hit.
    HAMAPi MF_00008. Thymidy_synth_bact.
    InterProi IPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view ]
    Pfami PF00303. Thymidylat_synt. 1 hit.
    [Graphical view ]
    PRINTSi PR00108. THYMDSNTHASE.
    SUPFAMi SSF55831. SSF55831. 1 hit.
    TIGRFAMsi TIGR03284. thym_sym. 2 hits.
    PROSITEi PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

    Entry informationi

    Entry nameiTYSY_CUPNH
    AccessioniPrimary (citable) accession number: Q0K889
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 26, 2008
    Last sequence update: February 26, 2008
    Last modified: October 1, 2014
    This is version 58 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3