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Q0K808 (SYQ_CUPNH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine--tRNA ligase

EC=6.1.1.18
Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS
Gene names
Name:glnS
Ordered Locus Names:H16_A2784
OrganismCupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha) [Complete proteome] [HAMAP]
Taxonomic identifier381666 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus

Protein attributes

Sequence length585 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP-Rule MF_00126

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00126

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00126.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutaminyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

glutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 585585Glutamine--tRNA ligase HAMAP-Rule MF_00126
PRO_1000095505

Regions

Motif51 – 6111"HIGH" region HAMAP-Rule MF_00126
Motif299 – 3035"KMSKS" region HAMAP-Rule MF_00126

Sites

Binding site3021ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0K808 [UniParc].

Last modified October 3, 2006. Version 1.
Checksum: 16C047BB5FCCF319

FASTA58566,386
        10         20         30         40         50         60 
MSHDNKPTDS TPAASNFLRS IIDQDLAAST YAGRQDKAGE PLPTVITRFP PEPNGYLHIG 

        70         80         90        100        110        120 
HAKSICLNFG LARDYGGRCH LRFDDTNPVK EDTEYVDSII DAVHWLGFSW DSEGKDGTRQ 

       130        140        150        160        170        180 
PHLYYASDYF DQLYAFAETL IERGAAYIDS QTAEQIAASR GNFSEPGKPS PYRERSVEEN 

       190        200        210        220        230        240 
LQLFRDMRAG KYADGEHVLR AKIDMTAPNI VMRDPVLYRI RHAHHHRTGD KWCIYPMYDF 

       250        260        270        280        290        300 
THCISDALEN ITHSLCTLEF ENNRPLYDWV LEHLRDSGVF RDPLPHQYEF ARLNLTYAIT 

       310        320        330        340        350        360 
SKRKLKQLVD EQRVDGWDDP RMPTLVGVRR RGYTPESIQL FCDRVGVAKA DSWIDMSTLE 

       370        380        390        400        410        420 
GAVRDDLDGR AARGVAVLDP LKLIIDNYPE GQSEECSAPV HPKKPELGKR VFPLSRELWI 

       430        440        450        460        470        480 
EREDFNETPP KGYFRLFPGN KVRLKYGYVI ECTGVDKDAD GNVIAVHASY LPETKSGTPG 

       490        500        510        520        530        540 
ADSVKVKGVI HWVSAAHAYE AEVRLYDRLF NDPNPDAGGK NFLDALNPDS KQVITAYLEP 

       550        560        570        580 
GLREAQPEDR FQFERHGYFV ADRTDSQPGK PVFNRIVGLK DSWGK 

« Hide

References

[1]"Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia eutropha H16."
Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I., Gottschalk G., Steinbuechel A., Friedrich B., Bowien B.
Nat. Biotechnol. 24:1257-1262(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM260479 Genomic DNA. Translation: CAJ93863.1.
RefSeqYP_727231.1. NC_008313.1.

3D structure databases

ProteinModelPortalQ0K808.
SMRQ0K808. Positions 15-582.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING381666.H16_A2784.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAJ93863; CAJ93863; H16_A2784.
GeneID4248422.
KEGGreh:H16_A2784.
PATRIC35235101. VBIRalEut6770_3182.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000259232.
KOK01886.
OMAKSDSWID.
OrthoDBEOG6DRPF7.

Enzyme and pathway databases

BioCycCNEC381666:GJUJ-2749-MONOMER.

Family and domain databases

Gene3D1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
HAMAPMF_00126. Gln_tRNA_synth.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR022861. Gln_tRNA_ligase_bac.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF50715. SSF50715. 1 hit.
TIGRFAMsTIGR00440. glnS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYQ_CUPNH
AccessionPrimary (citable) accession number: Q0K808
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: October 3, 2006
Last modified: May 14, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries