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Q0K7S5

- TRMB_CUPNH

UniProt

Q0K7S5 - TRMB_CUPNH

Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (03 Oct 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei91 – 911S-adenosyl-L-methionineUniRule annotation
    Binding sitei116 – 1161S-adenosyl-L-methionineUniRule annotation
    Binding sitei143 – 1431S-adenosyl-L-methionineUniRule annotation
    Active sitei166 – 1661By similarity
    Binding sitei166 – 1661S-adenosyl-L-methionineUniRule annotation
    Binding sitei170 – 1701SubstrateUniRule annotation
    Binding sitei202 – 2021SubstrateUniRule annotation

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciCNEC381666:GJUJ-2834-MONOMER.
    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
    tRNA(m7G46)-methyltransferaseUniRule annotation
    Gene namesi
    Name:trmBUniRule annotation
    Ordered Locus Names:H16_A2870
    OrganismiCupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
    Taxonomic identifieri381666 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
    ProteomesiUP000008210: Chromosome 1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 263263tRNA (guanine-N(7)-)-methyltransferasePRO_0000288207Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi381666.H16_A2870.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0K7S5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni172 – 1776Interaction with RNAUniRule annotation
    Regioni240 – 2434Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000073968.
    KOiK03439.
    OMAiRERANAK.
    OrthoDBiEOG6K6VBC.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q0K7S5-1 [UniParc]FASTAAdd to Basket

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    MLPQDPSTEP TPADDAAPVD SAGQASAPSP ADPEGVAHPR RIRSFVRRAG    50
    RTSTGQQRAI DEVGPRMMVP YAPQPLDWEA TFGRKAPSIL EIGFGMGETT 100
    AHIAGLRPQD NFLGCEVHEP GVGALLKLID ERGLANVRIL QHDAVEVIAH 150
    MLTDDCLDGV HIYFPDPWHK KRHNKRRLVQ PPLVKVLAAR LKPGGYIHCA 200
    TDWEEYAHQM LEVLSGEPLL ENTSTAADGF AERPDYRPVT KFERRGVRLG 250
    HGVWDVVFRK RAA 263
    Length:263
    Mass (Da):29,047
    Last modified:October 3, 2006 - v1
    Checksum:i28C58FFF3E1AE22B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM260479 Genomic DNA. Translation: CAJ93946.1.
    RefSeqiYP_727314.1. NC_008313.1.

    Genome annotation databases

    EnsemblBacteriaiCAJ93946; CAJ93946; H16_A2870.
    GeneIDi4250530.
    KEGGireh:H16_A2870.
    PATRICi35235277. VBIRalEut6770_3268.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM260479 Genomic DNA. Translation: CAJ93946.1 .
    RefSeqi YP_727314.1. NC_008313.1.

    3D structure databases

    ProteinModelPortali Q0K7S5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 381666.H16_A2870.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAJ93946 ; CAJ93946 ; H16_A2870 .
    GeneIDi 4250530.
    KEGGi reh:H16_A2870.
    PATRICi 35235277. VBIRalEut6770_3268.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000073968.
    KOi K03439.
    OMAi RERANAK.
    OrthoDBi EOG6K6VBC.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .
    BioCyci CNEC381666:GJUJ-2834-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

    Entry informationi

    Entry nameiTRMB_CUPNH
    AccessioniPrimary (citable) accession number: Q0K7S5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 29, 2007
    Last sequence update: October 3, 2006
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3