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Q0K7A0

- SYI_CUPNH

UniProt

Q0K7A0 - SYI_CUPNH

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Protein

Isoleucine--tRNA ligase

Gene
ileS, H16_A3046
Organism
Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei596 – 5961Aminoacyl-adenylate By similarity
Binding sitei640 – 6401ATP By similarity
Metal bindingi927 – 9271Zinc By similarity
Metal bindingi930 – 9301Zinc By similarity
Metal bindingi947 – 9471Zinc By similarity
Metal bindingi950 – 9501Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCNEC381666:GJUJ-3010-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:H16_A3046
OrganismiCupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
Taxonomic identifieri381666 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
ProteomesiUP000008210: Chromosome 1

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 964964Isoleucine--tRNA ligaseUniRule annotationPRO_1000022111Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi381666.H16_A3046.

Structurei

3D structure databases

ProteinModelPortaliQ0K7A0.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi66 – 7611"HIGH" regionUniRule annotationAdd
BLAST
Motifi637 – 6415"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiERLMLHQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0K7A0-1 [UniParc]FASTAAdd to Basket

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MPDDKRAKPE KSKYPVNLLD TPFPMRGDLP KREPQWVKQW QDKQLYKKIR    50
AARKGAKKFV LHDGPPYANG DIHIGHAVNK VLKDMIIKAR GLTGLDAVYV 100
PGWDCHGMPI EIQIEKKFGK GLPVQEVQAK ARAYATEQIK RQMMDFERLG 150
VLGDWDHPYL TMNYSNEADE LRALGKIMEK GYVFRGLKPV NWCFDCGSAL 200
AEAEVEYKDK VDLSIDVGFP FAQADKLAHA FKVPFEQLDG KPGWIVIWTT 250
TPWTIPSNQA LNVHPEVEYA LVETPRGYLI LATERVEEQL KVYGLEGKVV 300
ATTTGAALSE IRFHHPLAKM DAGYDRLSPV YLGDYVTTDT GSGIVHSAPA 350
YGVEDFQSCK AHGMADSDII SPVMGNGVYA GTLPLFGGLS IWDANPKIVE 400
VLQASGNLFN SHKYTHSYMH CWRHKTPIIY RATSQWFAGM DVDPADENGK 450
TGPTLRETAL AGIDATEFFP AWGKQRLHNM IANRPDWTLS RQRQWGVPMA 500
FFLHKETGAL HPRTPQLLEE VARRVEQHGI EAWQTLDPAE LLGDEASQYE 550
KNRDTLDVWF DSGTTHWSVI RGSHRDDLYD PSADEADGRL ADLYLEGSDQ 600
HRGWFHSSLL TASMLYGKPP YKALLTHGFT VDGEGRKMSK SIGNTVSPQD 650
ISNKMGAEII RLWVASTDYS GELSISDEIL KRVVEGYRRI RNTLRFLLSN 700
LSDYDHARHA LPASEWLEID RYAVALTAQL QKEVLSHYEA YEFHPVVSKL 750
QTFCSEDLGG FYLDVLKDRL YTTAPDSKAR RAAQNALYHI TQAMLHWMAP 800
FLSFTAEEAW QIFAHGTEHT DTIFTSTYYA IPEVDDGADD LLQKWHTLRE 850
VRAEVTKQLE AVRVEGEIGS SLQAELTIQA GGPVLDALQS LGDDLRFVLL 900
TSSAKVTHAP EAGDLLVTVT PSTHAKCERC WHYRADVGHN PDHPTLCGRC 950
DSNLFGAGEH RSHA 964
Length:964
Mass (Da):108,212
Last modified:October 3, 2006 - v1
Checksum:i082E30A7D7350B97
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM260479 Genomic DNA. Translation: CAJ94121.1.
RefSeqiYP_727489.1. NC_008313.1.

Genome annotation databases

EnsemblBacteriaiCAJ94121; CAJ94121; H16_A3046.
GeneIDi4250185.
KEGGireh:H16_A3046.
PATRICi35235637. VBIRalEut6770_3447.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM260479 Genomic DNA. Translation: CAJ94121.1 .
RefSeqi YP_727489.1. NC_008313.1.

3D structure databases

ProteinModelPortali Q0K7A0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 381666.H16_A3046.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAJ94121 ; CAJ94121 ; H16_A3046 .
GeneIDi 4250185.
KEGGi reh:H16_A3046.
PATRICi 35235637. VBIRalEut6770_3447.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi ERLMLHQ.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CNEC381666:GJUJ-3010-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

Entry informationi

Entry nameiSYI_CUPNH
AccessioniPrimary (citable) accession number: Q0K7A0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 3, 2006
Last modified: May 14, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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