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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei596 – 5961Aminoacyl-adenylateUniRule annotation
Binding sitei640 – 6401ATPUniRule annotation
Metal bindingi927 – 9271ZincUniRule annotation
Metal bindingi930 – 9301ZincUniRule annotation
Metal bindingi947 – 9471ZincUniRule annotation
Metal bindingi950 – 9501ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCNEC381666:GJUJ-3010-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:H16_A3046
OrganismiCupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
Taxonomic identifieri381666 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
ProteomesiUP000008210 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 964964Isoleucine--tRNA ligasePRO_1000022111Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi381666.H16_A3046.

Structurei

3D structure databases

ProteinModelPortaliQ0K7A0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi66 – 7611"HIGH" regionAdd
BLAST
Motifi637 – 6415"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0K7A0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPDDKRAKPE KSKYPVNLLD TPFPMRGDLP KREPQWVKQW QDKQLYKKIR
60 70 80 90 100
AARKGAKKFV LHDGPPYANG DIHIGHAVNK VLKDMIIKAR GLTGLDAVYV
110 120 130 140 150
PGWDCHGMPI EIQIEKKFGK GLPVQEVQAK ARAYATEQIK RQMMDFERLG
160 170 180 190 200
VLGDWDHPYL TMNYSNEADE LRALGKIMEK GYVFRGLKPV NWCFDCGSAL
210 220 230 240 250
AEAEVEYKDK VDLSIDVGFP FAQADKLAHA FKVPFEQLDG KPGWIVIWTT
260 270 280 290 300
TPWTIPSNQA LNVHPEVEYA LVETPRGYLI LATERVEEQL KVYGLEGKVV
310 320 330 340 350
ATTTGAALSE IRFHHPLAKM DAGYDRLSPV YLGDYVTTDT GSGIVHSAPA
360 370 380 390 400
YGVEDFQSCK AHGMADSDII SPVMGNGVYA GTLPLFGGLS IWDANPKIVE
410 420 430 440 450
VLQASGNLFN SHKYTHSYMH CWRHKTPIIY RATSQWFAGM DVDPADENGK
460 470 480 490 500
TGPTLRETAL AGIDATEFFP AWGKQRLHNM IANRPDWTLS RQRQWGVPMA
510 520 530 540 550
FFLHKETGAL HPRTPQLLEE VARRVEQHGI EAWQTLDPAE LLGDEASQYE
560 570 580 590 600
KNRDTLDVWF DSGTTHWSVI RGSHRDDLYD PSADEADGRL ADLYLEGSDQ
610 620 630 640 650
HRGWFHSSLL TASMLYGKPP YKALLTHGFT VDGEGRKMSK SIGNTVSPQD
660 670 680 690 700
ISNKMGAEII RLWVASTDYS GELSISDEIL KRVVEGYRRI RNTLRFLLSN
710 720 730 740 750
LSDYDHARHA LPASEWLEID RYAVALTAQL QKEVLSHYEA YEFHPVVSKL
760 770 780 790 800
QTFCSEDLGG FYLDVLKDRL YTTAPDSKAR RAAQNALYHI TQAMLHWMAP
810 820 830 840 850
FLSFTAEEAW QIFAHGTEHT DTIFTSTYYA IPEVDDGADD LLQKWHTLRE
860 870 880 890 900
VRAEVTKQLE AVRVEGEIGS SLQAELTIQA GGPVLDALQS LGDDLRFVLL
910 920 930 940 950
TSSAKVTHAP EAGDLLVTVT PSTHAKCERC WHYRADVGHN PDHPTLCGRC
960
DSNLFGAGEH RSHA
Length:964
Mass (Da):108,212
Last modified:October 3, 2006 - v1
Checksum:i082E30A7D7350B97
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM260479 Genomic DNA. Translation: CAJ94121.1.
RefSeqiWP_011615965.1. NC_008313.1.

Genome annotation databases

EnsemblBacteriaiCAJ94121; CAJ94121; H16_A3046.
GeneIDi4250185.
KEGGireh:H16_A3046.
PATRICi35235637. VBIRalEut6770_3447.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM260479 Genomic DNA. Translation: CAJ94121.1.
RefSeqiWP_011615965.1. NC_008313.1.

3D structure databases

ProteinModelPortaliQ0K7A0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi381666.H16_A3046.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ94121; CAJ94121; H16_A3046.
GeneIDi4250185.
KEGGireh:H16_A3046.
PATRICi35235637. VBIRalEut6770_3447.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciCNEC381666:GJUJ-3010-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

Entry informationi

Entry nameiSYI_CUPNH
AccessioniPrimary (citable) accession number: Q0K7A0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 3, 2006
Last modified: July 22, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.