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Q0K7A0

- SYI_CUPNH

UniProt

Q0K7A0 - SYI_CUPNH

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (03 Oct 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei596 – 5961Aminoacyl-adenylateUniRule annotation
    Binding sitei640 – 6401ATPUniRule annotation
    Metal bindingi927 – 9271ZincUniRule annotation
    Metal bindingi930 – 9301ZincUniRule annotation
    Metal bindingi947 – 9471ZincUniRule annotation
    Metal bindingi950 – 9501ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciCNEC381666:GJUJ-3010-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:H16_A3046
    OrganismiCupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
    Taxonomic identifieri381666 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
    ProteomesiUP000008210: Chromosome 1

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 964964Isoleucine--tRNA ligasePRO_1000022111Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi381666.H16_A3046.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0K7A0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi66 – 7611"HIGH" regionAdd
    BLAST
    Motifi637 – 6415"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiERLMLHQ.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q0K7A0-1 [UniParc]FASTAAdd to Basket

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    MPDDKRAKPE KSKYPVNLLD TPFPMRGDLP KREPQWVKQW QDKQLYKKIR    50
    AARKGAKKFV LHDGPPYANG DIHIGHAVNK VLKDMIIKAR GLTGLDAVYV 100
    PGWDCHGMPI EIQIEKKFGK GLPVQEVQAK ARAYATEQIK RQMMDFERLG 150
    VLGDWDHPYL TMNYSNEADE LRALGKIMEK GYVFRGLKPV NWCFDCGSAL 200
    AEAEVEYKDK VDLSIDVGFP FAQADKLAHA FKVPFEQLDG KPGWIVIWTT 250
    TPWTIPSNQA LNVHPEVEYA LVETPRGYLI LATERVEEQL KVYGLEGKVV 300
    ATTTGAALSE IRFHHPLAKM DAGYDRLSPV YLGDYVTTDT GSGIVHSAPA 350
    YGVEDFQSCK AHGMADSDII SPVMGNGVYA GTLPLFGGLS IWDANPKIVE 400
    VLQASGNLFN SHKYTHSYMH CWRHKTPIIY RATSQWFAGM DVDPADENGK 450
    TGPTLRETAL AGIDATEFFP AWGKQRLHNM IANRPDWTLS RQRQWGVPMA 500
    FFLHKETGAL HPRTPQLLEE VARRVEQHGI EAWQTLDPAE LLGDEASQYE 550
    KNRDTLDVWF DSGTTHWSVI RGSHRDDLYD PSADEADGRL ADLYLEGSDQ 600
    HRGWFHSSLL TASMLYGKPP YKALLTHGFT VDGEGRKMSK SIGNTVSPQD 650
    ISNKMGAEII RLWVASTDYS GELSISDEIL KRVVEGYRRI RNTLRFLLSN 700
    LSDYDHARHA LPASEWLEID RYAVALTAQL QKEVLSHYEA YEFHPVVSKL 750
    QTFCSEDLGG FYLDVLKDRL YTTAPDSKAR RAAQNALYHI TQAMLHWMAP 800
    FLSFTAEEAW QIFAHGTEHT DTIFTSTYYA IPEVDDGADD LLQKWHTLRE 850
    VRAEVTKQLE AVRVEGEIGS SLQAELTIQA GGPVLDALQS LGDDLRFVLL 900
    TSSAKVTHAP EAGDLLVTVT PSTHAKCERC WHYRADVGHN PDHPTLCGRC 950
    DSNLFGAGEH RSHA 964
    Length:964
    Mass (Da):108,212
    Last modified:October 3, 2006 - v1
    Checksum:i082E30A7D7350B97
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM260479 Genomic DNA. Translation: CAJ94121.1.
    RefSeqiYP_727489.1. NC_008313.1.

    Genome annotation databases

    EnsemblBacteriaiCAJ94121; CAJ94121; H16_A3046.
    GeneIDi4250185.
    KEGGireh:H16_A3046.
    PATRICi35235637. VBIRalEut6770_3447.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM260479 Genomic DNA. Translation: CAJ94121.1 .
    RefSeqi YP_727489.1. NC_008313.1.

    3D structure databases

    ProteinModelPortali Q0K7A0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 381666.H16_A3046.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAJ94121 ; CAJ94121 ; H16_A3046 .
    GeneIDi 4250185.
    KEGGi reh:H16_A3046.
    PATRICi 35235637. VBIRalEut6770_3447.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi ERLMLHQ.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci CNEC381666:GJUJ-3010-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

    Entry informationi

    Entry nameiSYI_CUPNH
    AccessioniPrimary (citable) accession number: Q0K7A0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: October 3, 2006
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3