Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei596Aminoacyl-adenylateUniRule annotation1
Binding sitei640ATPUniRule annotation1
Metal bindingi927ZincUniRule annotation1
Metal bindingi930ZincUniRule annotation1
Metal bindingi947ZincUniRule annotation1
Metal bindingi950ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:H16_A3046
OrganismiCupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
Taxonomic identifieri381666 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
Proteomesi
  • UP000008210 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000221111 – 964Isoleucine--tRNA ligaseAdd BLAST964

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi381666.H16_A3046

Structurei

3D structure databases

ProteinModelPortaliQ0K7A0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi66 – 76"HIGH" regionAdd BLAST11
Motifi637 – 641"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q0K7A0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPDDKRAKPE KSKYPVNLLD TPFPMRGDLP KREPQWVKQW QDKQLYKKIR
60 70 80 90 100
AARKGAKKFV LHDGPPYANG DIHIGHAVNK VLKDMIIKAR GLTGLDAVYV
110 120 130 140 150
PGWDCHGMPI EIQIEKKFGK GLPVQEVQAK ARAYATEQIK RQMMDFERLG
160 170 180 190 200
VLGDWDHPYL TMNYSNEADE LRALGKIMEK GYVFRGLKPV NWCFDCGSAL
210 220 230 240 250
AEAEVEYKDK VDLSIDVGFP FAQADKLAHA FKVPFEQLDG KPGWIVIWTT
260 270 280 290 300
TPWTIPSNQA LNVHPEVEYA LVETPRGYLI LATERVEEQL KVYGLEGKVV
310 320 330 340 350
ATTTGAALSE IRFHHPLAKM DAGYDRLSPV YLGDYVTTDT GSGIVHSAPA
360 370 380 390 400
YGVEDFQSCK AHGMADSDII SPVMGNGVYA GTLPLFGGLS IWDANPKIVE
410 420 430 440 450
VLQASGNLFN SHKYTHSYMH CWRHKTPIIY RATSQWFAGM DVDPADENGK
460 470 480 490 500
TGPTLRETAL AGIDATEFFP AWGKQRLHNM IANRPDWTLS RQRQWGVPMA
510 520 530 540 550
FFLHKETGAL HPRTPQLLEE VARRVEQHGI EAWQTLDPAE LLGDEASQYE
560 570 580 590 600
KNRDTLDVWF DSGTTHWSVI RGSHRDDLYD PSADEADGRL ADLYLEGSDQ
610 620 630 640 650
HRGWFHSSLL TASMLYGKPP YKALLTHGFT VDGEGRKMSK SIGNTVSPQD
660 670 680 690 700
ISNKMGAEII RLWVASTDYS GELSISDEIL KRVVEGYRRI RNTLRFLLSN
710 720 730 740 750
LSDYDHARHA LPASEWLEID RYAVALTAQL QKEVLSHYEA YEFHPVVSKL
760 770 780 790 800
QTFCSEDLGG FYLDVLKDRL YTTAPDSKAR RAAQNALYHI TQAMLHWMAP
810 820 830 840 850
FLSFTAEEAW QIFAHGTEHT DTIFTSTYYA IPEVDDGADD LLQKWHTLRE
860 870 880 890 900
VRAEVTKQLE AVRVEGEIGS SLQAELTIQA GGPVLDALQS LGDDLRFVLL
910 920 930 940 950
TSSAKVTHAP EAGDLLVTVT PSTHAKCERC WHYRADVGHN PDHPTLCGRC
960
DSNLFGAGEH RSHA
Length:964
Mass (Da):108,212
Last modified:October 3, 2006 - v1
Checksum:i082E30A7D7350B97
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM260479 Genomic DNA Translation: CAJ94121.1
RefSeqiWP_011615965.1, NC_008313.1

Genome annotation databases

EnsemblBacteriaiCAJ94121; CAJ94121; H16_A3046
KEGGireh:H16_A3046
PATRICifig|381666.6.peg.3447

Similar proteinsi

Entry informationi

Entry nameiSYI_CUPNH
AccessioniPrimary (citable) accession number: Q0K7A0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 3, 2006
Last modified: March 28, 2018
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health