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Q0K6M6 (DDL_CUPNH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:H16_A3271
OrganismCupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha) [Complete proteome] [HAMAP]
Taxonomic identifier381666 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus

Protein attributes

Sequence length327 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 327327D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341158

Regions

Domain113 – 312200ATP-grasp
Nucleotide binding139 – 19456ATP By similarity

Sites

Metal binding2661Magnesium or manganese 1 By similarity
Metal binding2791Magnesium or manganese 1 By similarity
Metal binding2791Magnesium or manganese 2 By similarity
Metal binding2811Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0K6M6 [UniParc].

Last modified October 3, 2006. Version 1.
Checksum: 7A1E82D8D444FFD7

FASTA32734,924
        10         20         30         40         50         60 
MSFVAHPNID PKSLGKVGVL LGGRSAEREI SLMSGNGVLA ALQSRGVDAH GFDPGLQSVA 

        70         80         90        100        110        120 
ELAAAGFDRV FIALHGRYGE DGTIQGLLEQ LGVPYTGSGV LASAMAMDKQ ATKRLWMTYG 

       130        140        150        160        170        180 
LATPRFAMLH ADTDFDAVAA DLGLPLIVKP AREGSSIGLT KVTAADQMRA AFEKAAALDN 

       190        200        210        220        230        240 
DVIAETFIDG AELTCPIVGE GDSAEALPVI RIVAPEANYD YQNKYFTDDT QYLCPSGLDP 

       250        260        270        280        290        300 
EVEREVRALA VQSYRVLGCR GWARADLMLR ADGKPFLLEM NTSPGMTGHS LVPMAARAVG 

       310        320 
ISYEDFVMQV VAAATLDLHP NEHWKPE 

« Hide

References

[1]"Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia eutropha H16."
Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I., Gottschalk G., Steinbuechel A., Friedrich B., Bowien B.
Nat. Biotechnol. 24:1257-1262(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM260479 Genomic DNA. Translation: CAJ94345.1.
RefSeqYP_727713.1. NC_008313.1.

3D structure databases

ProteinModelPortalQ0K6M6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING381666.H16_A3271.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAJ94345; CAJ94345; H16_A3271.
GeneID4246932.
KEGGreh:H16_A3271.
PATRIC35236075. VBIRalEut6770_3665.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMADTQYRIP.
OrthoDBEOG6ND0KB.
ProtClustDBPRK01372.

Enzyme and pathway databases

BioCycCNEC381666:GJUJ-3235-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_CUPNH
AccessionPrimary (citable) accession number: Q0K6M6
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: October 3, 2006
Last modified: February 19, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways