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Protein

Bifunctional ribose 1,5-bisphosphokinase-thymidine phosphorylase

Gene

phnN

Organism
Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of ribose 1,5-bisphosphate to 5-phospho-D-ribosyl alpha-1-diphosphate (PRPP).By similarity

Catalytic activityi

ATP + alpha-D-ribose 1,5-bisphosphate = ADP + 5-phospho-alpha-D-ribose 1-diphosphate.
Thymidine + phosphate = thymine + 2-deoxy-alpha-D-ribose 1-phosphate.

Pathwayi: 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route II).
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. Bifunctional ribose 1,5-bisphosphokinase-thymidine phosphorylase (phnN), Ribose 1,5-bisphosphate phosphokinase PhnN (phnN)
This subpathway is part of the pathway 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route II), the pathway 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis and in Metabolic intermediate biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Alkylphosphonate uptake

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00087; UER00175.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional ribose 1,5-bisphosphokinase-thymidine phosphorylase
Including the following 2 domains:
Ribose 1,5-bisphosphate phosphokinase PhnN (EC:2.7.4.23)
Alternative name(s):
Ribose 1,5-bisphosphokinase
Putative thymidine phosphorylase (EC:2.4.2.4)
Alternative name(s):
TdRPase
Gene namesi
Name:phnN
Ordered Locus Names:H16_B0558
OrganismiCupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
Taxonomic identifieri381666 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
Proteomesi
  • UP000008210 Componenti: Chromosome 2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003147091 – 601Bifunctional ribose 1,5-bisphosphokinase-thymidine phosphorylaseAdd BLAST601

Proteomic databases

PRIDEiQ0K3R9.

Interactioni

Protein-protein interaction databases

STRINGi381666.H16_B0558.

Structurei

3D structure databases

ProteinModelPortaliQ0K3R9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 177Ribose 1,5-bisphosphokinaseAdd BLAST177
Regioni178 – 601ThymidinephosphorylaseAdd BLAST424

Sequence similaritiesi

In the N-terminal section; belongs to the ribose 1,5-bisphosphokinase family.Curated
In the C-terminal section; belongs to the thymidine/pyrimidine-nucleoside phosphorylase family. Type 2 subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105CMW. Bacteria.
COG0213. LUCA.
COG3709. LUCA.
HOGENOMiHOG000252767.
KOiK00758.
OMAiEVGCCIV.

Family and domain databases

Gene3Di3.40.1030.10. 1 hit.
3.40.50.300. 2 hits.
3.90.1170.30. 1 hit.
HAMAPiMF_00836. PhnN. 1 hit.
MF_00703. Thymid_phosp_2. 1 hit.
InterProiIPR000312. Glycosyl_Trfase_fam3.
IPR027417. P-loop_NTPase.
IPR012699. PhnN.
IPR013102. PYNP_C.
IPR017872. Pyrmidine_PPase_CS.
IPR028579. Thym_Pase_Put.
IPR000053. Thymidine/pyrmidine_PPase.
[Graphical view]
PANTHERiPTHR10515. PTHR10515. 1 hit.
PfamiPF00591. Glycos_transf_3. 1 hit.
PF07831. PYNP_C. 1 hit.
[Graphical view]
SMARTiSM00941. PYNP_C. 1 hit.
[Graphical view]
SUPFAMiSSF52418. SSF52418. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54680. SSF54680. 1 hit.
TIGRFAMsiTIGR02322. phosphon_PhnN. 1 hit.
PROSITEiPS00647. THYMID_PHOSPHORYLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0K3R9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKATGTFFFV VGPSGAGKDS LIDGARAALD GDYVFARRVI TRPDGSAGEE
60 70 80 90 100
HEGVTEAEFA RRQRSGEFLV TWDAHDLRYG LPKSLMCELE RGRNVVANGS
110 120 130 140 150
RGVIAELAAR LPRFVVVLVT APHDVLARRI AARGRESGDQ VASRVARAGA
160 170 180 190 200
PVPPHVPCIT VSNHSTLEAG TARFVEALRT GTRTSAAERP ASRTNLMAKL
210 220 230 240 250
RGEPLDEAAY VAVLQDAIAG RYTEAELTEF LVAATRTLTD EEVVALARAR
260 270 280 290 300
TAFTPRIDWD EPVVVDKHSM GGVPGSRITL IVVPIVAAYG LAMPKTSSRA
310 320 330 340 350
ITSAAGTADA METIARVDLA HEDVRRCVAQ ARACIAWNGR LNHSVVDDVM
360 370 380 390 400
NAITRPLRLD SRRWSVASIL SKKYTAGATH VIVDLPYGPQ TKLATRADAE
410 420 430 440 450
ALGALFEHVG KGLGLHVRAL VTDGSGPIGR GIGPALEVRD VRLVLDNAPD
460 470 480 490 500
APADLRDKAL RFAGEIIAFD PRVDSPEHGM QIAAALLHEG KARAAFDRIA
510 520 530 540 550
AAQGVRCDPV APGTHTLVVP ATTRGRVAGV DGLQISGVAR AAGAPRDGGA
560 570 580 590 600
GVDMLCAIGA KVAPGQPLYR IHSDSAEALE AAAALVRAGG ECCQAVRIDP

D
Length:601
Mass (Da):63,353
Last modified:October 3, 2006 - v1
Checksum:i99B7554ACC47C203
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM260480 Genomic DNA. Translation: CAJ95355.1.
RefSeqiWP_011616677.1. NC_008314.1.

Genome annotation databases

EnsemblBacteriaiCAJ95355; CAJ95355; H16_B0558.
GeneIDi4456323.
KEGGireh:H16_B0558.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM260480 Genomic DNA. Translation: CAJ95355.1.
RefSeqiWP_011616677.1. NC_008314.1.

3D structure databases

ProteinModelPortaliQ0K3R9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi381666.H16_B0558.

Proteomic databases

PRIDEiQ0K3R9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ95355; CAJ95355; H16_B0558.
GeneIDi4456323.
KEGGireh:H16_B0558.

Phylogenomic databases

eggNOGiENOG4105CMW. Bacteria.
COG0213. LUCA.
COG3709. LUCA.
HOGENOMiHOG000252767.
KOiK00758.
OMAiEVGCCIV.

Enzyme and pathway databases

UniPathwayiUPA00087; UER00175.

Family and domain databases

Gene3Di3.40.1030.10. 1 hit.
3.40.50.300. 2 hits.
3.90.1170.30. 1 hit.
HAMAPiMF_00836. PhnN. 1 hit.
MF_00703. Thymid_phosp_2. 1 hit.
InterProiIPR000312. Glycosyl_Trfase_fam3.
IPR027417. P-loop_NTPase.
IPR012699. PhnN.
IPR013102. PYNP_C.
IPR017872. Pyrmidine_PPase_CS.
IPR028579. Thym_Pase_Put.
IPR000053. Thymidine/pyrmidine_PPase.
[Graphical view]
PANTHERiPTHR10515. PTHR10515. 1 hit.
PfamiPF00591. Glycos_transf_3. 1 hit.
PF07831. PYNP_C. 1 hit.
[Graphical view]
SMARTiSM00941. PYNP_C. 1 hit.
[Graphical view]
SUPFAMiSSF52418. SSF52418. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54680. SSF54680. 1 hit.
TIGRFAMsiTIGR02322. phosphon_PhnN. 1 hit.
PROSITEiPS00647. THYMID_PHOSPHORYLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRBKTP_CUPNH
AccessioniPrimary (citable) accession number: Q0K3R9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 3, 2006
Last modified: November 2, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.