Q0K3R9 (RBKTP_CUPNH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional ribose 1,5-bisphosphokinase-thymidine phosphorylase

Including the following 2 domains:

1. Ribose 1,5-bisphosphate phosphokinase PhnN
   EC=2.7.4.23
   Alternative name(s):
   Ribose 1,5-bisphosphokinase
2. Putative thymidine phosphorylase
   EC=2.4.2.4
   Alternative name(s):
   TdRPase

Gene names

Name:	phnN
Ordered Locus Names:	H16_B0558

Organism

Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha) [Complete proteome] [HAMAP]

Taxonomic identifier

381666 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus › Cupriavidus necator

Protein attributes

Sequence length	601 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of ribose 1,5-bisphosphate to 5-phospho-D-ribosyl alpha-1-diphosphate (PRPP) By similarity. HAMAP-Rule MF_00703
Catalytic activity	ATP + alpha-D-ribose 1,5-bisphosphate = ADP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP-Rule MF_00703 Thymidine + phosphate = thymine + 2-deoxy-alpha-D-ribose 1-phosphate. HAMAP-Rule MF_00703
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route II): step 3/3. HAMAP-Rule MF_00703
Sequence similarities	In the N-terminal section; belongs to the ribose 1,5-bisphosphokinase family. In the C-terminal section; belongs to the thymidine/pyrimidine-nucleoside phosphorylase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Alkylphosphonate uptake
Ligand	ATP-binding Nucleotide-binding
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological_process	5-phosphoribose 1-diphosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway organic phosphonate transport Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleobase metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleoside metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphorylase activity Inferred from electronic annotation. Source: InterPro ribose 1,5-bisphosphate phosphokinase activity Inferred from electronic annotation. Source: EC thymidine phosphorylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 601	601	Bifunctional ribose 1,5-bisphosphokinase-thymidine phosphorylase HAMAP-Rule MF_00703		PRO_0000314709
Regions
	Region	1 – 177	177	Ribose 1,5-bisphosphokinase HAMAP-Rule MF_00703
	Region	178 – 601	424	Thymidinephosphorylase HAMAP-Rule MF_00703

Sequences

	Sequence		Length	Mass (Da)
	Q0K3R9 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: 99B7554ACC47C203	FASTA	601	63,353
	10 20 30 40 50 60 MKATGTFFFV VGPSGAGKDS LIDGARAALD GDYVFARRVI TRPDGSAGEE HEGVTEAEFA 70 80 90 100 110 120 RRQRSGEFLV TWDAHDLRYG LPKSLMCELE RGRNVVANGS RGVIAELAAR LPRFVVVLVT 130 140 150 160 170 180 APHDVLARRI AARGRESGDQ VASRVARAGA PVPPHVPCIT VSNHSTLEAG TARFVEALRT 190 200 210 220 230 240 GTRTSAAERP ASRTNLMAKL RGEPLDEAAY VAVLQDAIAG RYTEAELTEF LVAATRTLTD 250 260 270 280 290 300 EEVVALARAR TAFTPRIDWD EPVVVDKHSM GGVPGSRITL IVVPIVAAYG LAMPKTSSRA 310 320 330 340 350 360 ITSAAGTADA METIARVDLA HEDVRRCVAQ ARACIAWNGR LNHSVVDDVM NAITRPLRLD 370 380 390 400 410 420 SRRWSVASIL SKKYTAGATH VIVDLPYGPQ TKLATRADAE ALGALFEHVG KGLGLHVRAL 430 440 450 460 470 480 VTDGSGPIGR GIGPALEVRD VRLVLDNAPD APADLRDKAL RFAGEIIAFD PRVDSPEHGM 490 500 510 520 530 540 QIAAALLHEG KARAAFDRIA AAQGVRCDPV APGTHTLVVP ATTRGRVAGV DGLQISGVAR 550 560 570 580 590 600 AAGAPRDGGA GVDMLCAIGA KVAPGQPLYR IHSDSAEALE AAAALVRAGG ECCQAVRIDP D

References

[1]

"Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia eutropha H16." Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I., Gottschalk G., Steinbuechel A., Friedrich B., Bowien B. Nat. Biotechnol. 24:1257-1262(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM260480 Genomic DNA. Translation: CAJ95355.1.
RefSeq	YP_728720.1. NC_008314.1.
3D structure databases
ProteinModelPortal	Q0K3R9.
ModBase	Search...
Protein-protein interaction databases
STRING	381666.H16_B0558.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAJ95355; CAJ95355; H16_B0558.
GeneID	4456323.
KEGG	reh:H16_B0558.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0213.
HOGENOM	HOG000252767.
KO	K00758.
OMA	VGDHFGI.
ProtClustDB	PRK04350.
Enzyme and pathway databases
BioCyc	CNEC381666:GJUJ-4265-MONOMER.
UniPathway	UPA00087; UER00175.
Family and domain databases
Gene3D	3.40.1030.10. 1 hit. 3.90.1170.30. 1 hit.
HAMAP	MF_00703. Thymid_phosp_2. Fused. MF_00836. PhnN. Fused.
InterPro	IPR000312. Glycosyl_Trfase_fam3. IPR012699. PhnN. IPR013102. PYNP_C. IPR000053. Pyrmidine_PPase. IPR017872. Pyrmidine_PPase_CS. IPR013466. Thymidine_Pase_type2. [Graphical view]
PANTHER	PTHR10515. PTHR10515. 1 hit.
Pfam	PF00591. Glycos_transf_3. 1 hit. PF07831. PYNP_C. 1 hit. [Graphical view]
SMART	SM00941. PYNP_C. 1 hit. [Graphical view]
SUPFAM	SSF52418. Glyco_trans_3. 1 hit. SSF54680. PYNP_C. 1 hit.
TIGRFAMs	TIGR02322. phosphon_PhnN. 1 hit.
PROSITE	PS50052. GUANYLATE_KINASE_2. False negative. PS00647. THYMID_PHOSPHORYLASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RBKTP_CUPNH

Accession

Primary (citable) accession number: Q0K3R9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 3, 2006
Last modified:	May 1, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program