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Protein

Aconitate hydratase B

Gene

acnB

Organism
Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = (Z)-but-2-ene-1,2,3-tricarboxylate + H2O.UniRule annotation
Citrate = isocitrate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 2 of the subpathway that synthesizes isocitrate from oxaloacetate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Citrate synthase (cisY)
  2. Aconitate hydratase B (acnB)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei191SubstrateUniRule annotation1
Binding sitei501SubstrateUniRule annotation1
Metal bindingi713Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi771Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi774Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei793SubstrateUniRule annotation1
Binding sitei798SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationSAAS annotationImported
Biological processTricarboxylic acid cycleUniRule annotation
Ligand4Fe-4SUniRule annotationSAAS annotation, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00223; UER00718

Names & Taxonomyi

Protein namesi
Recommended name:
Aconitate hydratase BUniRule annotation (EC:4.2.1.3UniRule annotation, EC:4.2.1.99UniRule annotation)
Alternative name(s):
2-methylisocitrate dehydrataseUniRule annotation
Gene namesi
Name:acnBImported
Ordered Locus Names:H16_B0568Imported
OrganismiCupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)Imported
Taxonomic identifieri381666 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
Proteomesi
  • UP000008210 Componenti: Chromosome 2

Subcellular locationi

GO - Cellular componenti

Interactioni

Protein-protein interaction databases

STRINGi381666.H16_B0568

Structurei

3D structure databases

ProteinModelPortaliQ0K3Q9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 156Aconitase_B_NInterPro annotationAdd BLAST153
Domaini168 – 382Aconitase_2_NInterPro annotationAdd BLAST215
Domaini475 – 817AconitaseInterPro annotationAdd BLAST343

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni246 – 248Substrate bindingUniRule annotation3
Regioni417 – 419Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the aconitase/IPM isomerase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QIJ Bacteria
COG1049 LUCA
HOGENOMiHOG000205991
KOiK01682
OMAiQDTTGAM

Family and domain databases

CDDicd01576 AcnB_Swivel, 1 hit
Gene3Di1.25.40.310, 1 hit
3.20.19.10, 1 hit
3.30.499.10, 2 hits
3.40.1060.10, 2 hits
InterProiView protein in InterPro
IPR015931 Acnase/IPM_dHydase_lsu_aba_1/3
IPR001030 Acoase/IPM_deHydtase_lsu_aba
IPR015928 Aconitase/3IPM_dehydase_swvl
IPR018136 Aconitase_4Fe-4S_BS
IPR036008 Aconitase_4Fe-4S_dom
IPR004406 Aconitase_B
IPR015933 Aconitase_B_HEAT-like_dom
IPR036288 Aconitase_B_HEAT-like_dom_sf
IPR015929 Aconitase_B_swivel
IPR015932 Aconitase_dom2
PANTHERiPTHR43160:SF1 PTHR43160:SF1, 1 hit
PfamiView protein in Pfam
PF00330 Aconitase, 1 hit
PF06434 Aconitase_2_N, 1 hit
PF11791 Aconitase_B_N, 1 hit
PIRSFiPIRSF036687 AcnB, 1 hit
SUPFAMiSSF53732 SSF53732, 1 hit
SSF74778 SSF74778, 1 hit
TIGRFAMsiTIGR00117 acnB, 1 hit
PROSITEiView protein in PROSITE
PS00450 ACONITASE_1, 1 hit
PS01244 ACONITASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q0K3Q9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLENYRAHVA ERAALGIPPL PLTAKQTAEL VELLKNPPAG EEQTLVELIT
60 70 80 90 100
YRVPAGVDDA AKVKASYLAA VALGKEKCAL ISRAKATELL GTMLGGYNIS
110 120 130 140 150
PLIELLDDAE VGTVAAEALK KTLLMFDAFH DVKEKADQGN ANAKAVLQSW
160 170 180 190 200
ADAEWFTSRP EVPQSLTITV FKVPGETNTD DLSPAPDATT RPDIPMHALA
210 220 230 240 250
MLKNKREGAA FQPEEDGKRG PVKFIESLKE KGHLVAYVGD VVGTGSSRKS
260 270 280 290 300
ATNSVLWFTG EDIPFIPNKR FGGVCLGNKI APIFYNTMED AGALPIELDV
310 320 330 340 350
SKMEMGDVVE LRPYEGKALK DGAVIAEFKV KSDVLFDEVR AGGRIPLIVG
360 370 380 390 400
RGLTAKAREA LGLAPSTLFR LPQNPADTGR GFTLAQKMVG RACGLPEGKG
410 420 430 440 450
IRPGTYCEPK MTSVGSQDTT GPMTRDELKD LACLGFSADL VMQSFCHTAA
460 470 480 490 500
YPKPVDVKTH HTLPEFISTR GGISLRPGDG VIHSWLNRML LPDTVGTGGD
510 520 530 540 550
SHTRFPIGIS FPAGSGLVAF AAATGVMPLD MPESVLVRFK GKMQPGVTLR
560 570 580 590 600
DLVNAIPLYA IKQGLLTVAK QGKKNIFSGR VLEIEGLPDL KVEQAFELSD
610 620 630 640 650
ASAERSAAGC TVRLNKDPII EYINSNITLL KWMIAQGYQD PRSLQRRIKA
660 670 680 690 700
MEAWLADPKL LEPDADAEYA AVIEIDLADV HEPIVACPND PDDVKTLSEV
710 720 730 740 750
AGAKIDEVFI GSCMTNIGHF RAASKLLEGK RDIPVKLWVA PPTKMDQKQL
760 770 780 790 800
TEEGHYGVFG TAGARTEMPG CSLCMGNQAQ VREGATVMST STRNFPNRLG
810 820 830 840 850
KNTNVYLGSA ELAAICSRLG RIPTKEEYMA DMGVLNANGD KIYKYMNFDQ
860
IEDFKEVADG VTV
Length:863
Mass (Da):93,126
Last modified:October 3, 2006 - v1
Checksum:i6624228948299E1E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM260480 Genomic DNA Translation: CAJ95365.1
RefSeqiWP_010810392.1, NC_008314.1

Genome annotation databases

EnsemblBacteriaiCAJ95365; CAJ95365; H16_B0568
KEGGireh:H16_B0568

Similar proteinsi

Entry informationi

Entry nameiQ0K3Q9_CUPNH
AccessioniPrimary (citable) accession number: Q0K3Q9
Entry historyiIntegrated into UniProtKB/TrEMBL: October 3, 2006
Last sequence update: October 3, 2006
Last modified: March 28, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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