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Protein

Aconitate hydratase B

Gene

acnB

Organism
Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = (Z)-but-2-ene-1,2,3-tricarboxylate + H2O.UniRule annotation
Citrate = isocitrate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 2 of the subpathway that synthesizes isocitrate from oxaloacetate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Citrate synthase (cisY)
  2. Aconitate hydratase B (acnB)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei191SubstrateUniRule annotation1
Binding sitei501SubstrateUniRule annotation1
Metal bindingi713Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi771Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi774Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei793SubstrateUniRule annotation1
Binding sitei798SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotationSAAS annotationImported

Keywords - Biological processi

Tricarboxylic acid cycleUniRule annotation

Keywords - Ligandi

4Fe-4SUniRule annotationSAAS annotation, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00223; UER00718.

Names & Taxonomyi

Protein namesi
Recommended name:
Aconitate hydratase BUniRule annotation (EC:4.2.1.3UniRule annotation, EC:4.2.1.99UniRule annotation)
Alternative name(s):
2-methylisocitrate dehydrataseUniRule annotation
Gene namesi
Name:acnBImported
Ordered Locus Names:H16_B0568Imported
OrganismiCupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)Imported
Taxonomic identifieri381666 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
Proteomesi
  • UP000008210 Componenti: Chromosome 2

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Interactioni

Protein-protein interaction databases

STRINGi381666.H16_B0568.

Structurei

3D structure databases

ProteinModelPortaliQ0K3Q9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 156Aconitase_B_NInterPro annotationAdd BLAST153
Domaini168 – 382Aconitase_2_NInterPro annotationAdd BLAST215
Domaini475 – 817AconitaseInterPro annotationAdd BLAST343

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni246 – 248Substrate bindingUniRule annotation3
Regioni417 – 419Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the aconitase/IPM isomerase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QIJ. Bacteria.
COG1049. LUCA.
HOGENOMiHOG000205991.
KOiK01682.
OMAiHATTRPD.

Family and domain databases

CDDicd01576. AcnB_Swivel. 1 hit.
Gene3Di3.20.19.10. 1 hit.
3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
InterProiIPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015928. Aconitase/3IPM_dehydase_swvl.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
IPR004406. Aconitase_B.
IPR015933. Aconitase_B_HEAT-like_dom.
IPR015929. Aconitase_B_swivel.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00330. Aconitase. 1 hit.
PF06434. Aconitase_2_N. 1 hit.
PF11791. Aconitase_B_N. 1 hit.
[Graphical view]
PIRSFiPIRSF036687. AcnB. 1 hit.
SUPFAMiSSF52016. SSF52016. 1 hit.
SSF53732. SSF53732. 1 hit.
SSF74778. SSF74778. 1 hit.
TIGRFAMsiTIGR00117. acnB. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0K3Q9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLENYRAHVA ERAALGIPPL PLTAKQTAEL VELLKNPPAG EEQTLVELIT
60 70 80 90 100
YRVPAGVDDA AKVKASYLAA VALGKEKCAL ISRAKATELL GTMLGGYNIS
110 120 130 140 150
PLIELLDDAE VGTVAAEALK KTLLMFDAFH DVKEKADQGN ANAKAVLQSW
160 170 180 190 200
ADAEWFTSRP EVPQSLTITV FKVPGETNTD DLSPAPDATT RPDIPMHALA
210 220 230 240 250
MLKNKREGAA FQPEEDGKRG PVKFIESLKE KGHLVAYVGD VVGTGSSRKS
260 270 280 290 300
ATNSVLWFTG EDIPFIPNKR FGGVCLGNKI APIFYNTMED AGALPIELDV
310 320 330 340 350
SKMEMGDVVE LRPYEGKALK DGAVIAEFKV KSDVLFDEVR AGGRIPLIVG
360 370 380 390 400
RGLTAKAREA LGLAPSTLFR LPQNPADTGR GFTLAQKMVG RACGLPEGKG
410 420 430 440 450
IRPGTYCEPK MTSVGSQDTT GPMTRDELKD LACLGFSADL VMQSFCHTAA
460 470 480 490 500
YPKPVDVKTH HTLPEFISTR GGISLRPGDG VIHSWLNRML LPDTVGTGGD
510 520 530 540 550
SHTRFPIGIS FPAGSGLVAF AAATGVMPLD MPESVLVRFK GKMQPGVTLR
560 570 580 590 600
DLVNAIPLYA IKQGLLTVAK QGKKNIFSGR VLEIEGLPDL KVEQAFELSD
610 620 630 640 650
ASAERSAAGC TVRLNKDPII EYINSNITLL KWMIAQGYQD PRSLQRRIKA
660 670 680 690 700
MEAWLADPKL LEPDADAEYA AVIEIDLADV HEPIVACPND PDDVKTLSEV
710 720 730 740 750
AGAKIDEVFI GSCMTNIGHF RAASKLLEGK RDIPVKLWVA PPTKMDQKQL
760 770 780 790 800
TEEGHYGVFG TAGARTEMPG CSLCMGNQAQ VREGATVMST STRNFPNRLG
810 820 830 840 850
KNTNVYLGSA ELAAICSRLG RIPTKEEYMA DMGVLNANGD KIYKYMNFDQ
860
IEDFKEVADG VTV
Length:863
Mass (Da):93,126
Last modified:October 3, 2006 - v1
Checksum:i6624228948299E1E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM260480 Genomic DNA. Translation: CAJ95365.1.
RefSeqiWP_010810392.1. NC_008314.1.

Genome annotation databases

EnsemblBacteriaiCAJ95365; CAJ95365; H16_B0568.
GeneIDi4456731.
KEGGireh:H16_B0568.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM260480 Genomic DNA. Translation: CAJ95365.1.
RefSeqiWP_010810392.1. NC_008314.1.

3D structure databases

ProteinModelPortaliQ0K3Q9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi381666.H16_B0568.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ95365; CAJ95365; H16_B0568.
GeneIDi4456731.
KEGGireh:H16_B0568.

Phylogenomic databases

eggNOGiENOG4107QIJ. Bacteria.
COG1049. LUCA.
HOGENOMiHOG000205991.
KOiK01682.
OMAiHATTRPD.

Enzyme and pathway databases

UniPathwayiUPA00223; UER00718.

Family and domain databases

CDDicd01576. AcnB_Swivel. 1 hit.
Gene3Di3.20.19.10. 1 hit.
3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
InterProiIPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015928. Aconitase/3IPM_dehydase_swvl.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
IPR004406. Aconitase_B.
IPR015933. Aconitase_B_HEAT-like_dom.
IPR015929. Aconitase_B_swivel.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00330. Aconitase. 1 hit.
PF06434. Aconitase_2_N. 1 hit.
PF11791. Aconitase_B_N. 1 hit.
[Graphical view]
PIRSFiPIRSF036687. AcnB. 1 hit.
SUPFAMiSSF52016. SSF52016. 1 hit.
SSF53732. SSF53732. 1 hit.
SSF74778. SSF74778. 1 hit.
TIGRFAMsiTIGR00117. acnB. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiQ0K3Q9_CUPNH
AccessioniPrimary (citable) accession number: Q0K3Q9
Entry historyi
Integrated into UniProtKB/TrEMBL: October 3, 2006
Last sequence update: October 3, 2006
Last modified: November 30, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.