Reviewed,
UniProtKB/Swiss-Prot Q0K1E0 (RBL1C_RALEH)
Last modified
January 19, 2010.
Version 30.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Ribulose bisphosphate carboxylase large chain, chromosomal Short name=RuBisCO large subunit EC=4.1.1.39 | ||||||
| Gene names |
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| Organism | Ralstonia eutropha (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)) [Complete proteome] [HAMAP] | ||||||
| Taxonomic identifier | 381666 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus |
Protein attributes
| Sequence length | 486 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site By similarity. HAMAP MF_01338 |
| Catalytic activity | 2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O. HAMAP MF_01338 3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2. HAMAP MF_01338 |
| Cofactor | Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01338 |
| Subunit structure | Heterohexadecamer of 8 large chains and 8 small chains; disulfide-linked. The disulfide link is formed within the large subunit homodimers By similarity. HAMAP MF_01338 |
| Induction | Total RuBisCO activity (both chromosome and plasmid-derived enzyme) is high under lithoautotrophic growth conditions, intermediate when grown on fructose and poor when grown on pyruvate. HAMAP MF_01338 |
| Post-translational modification | The disulfide bond which can form between Cys-278 in the large chain dimeric partners within the hexadecamer appears to be associated with oxidative stress and protein turnover By similarity. HAMAP MF_01338 |
| Miscellaneous | The basic functional RuBisCO is composed of a large chain homodimer in a "head-to-tail" conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric "cap" on each end of the "barrel" By similarity. HAMAP MF_01338 |
| Sequence similarities | Belongs to the RuBisCO large chain family. Type I subfamily. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Calvin cycle Carbon dioxide fixation |
| Ligand | Magnesium Metal-binding |
| Molecular function | Lyase Monooxygenase Oxidoreductase |
| PTM | Disulfide bond |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW reductive pentose-phosphate cycleInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | plastid Inferred from electronic annotation. Source: InterPro |
| Molecular function | magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW monooxygenase activityInferred from electronic annotation. Source: UniProtKB-KW ribulose-bisphosphate carboxylase activityInferred from electronic annotation. Source: HAMAP |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 486 | 486 | Ribulose bisphosphate carboxylase large chain, chromosomal HAMAP MF_01338 | PRO_0000273254 | |||||
Sites | |||||||||
| Active site | 178 | 1 | Proton acceptor By similarity | ||||||
| Active site | 296 | 1 | Proton acceptor By similarity | ||||||
| Metal binding | 204 | 1 | Magnesium; via carbamate group By similarity | ||||||
| Metal binding | 206 | 1 | Magnesium By similarity | ||||||
| Metal binding | 207 | 1 | Magnesium By similarity | ||||||
| Binding site | 126 | 1 | Substrate; in homodimeric partner By similarity | ||||||
| Binding site | 176 | 1 | Substrate By similarity | ||||||
| Binding site | 180 | 1 | Substrate By similarity | ||||||
| Binding site | 297 | 1 | Substrate By similarity | ||||||
| Binding site | 329 | 1 | Substrate By similarity | ||||||
| Binding site | 381 | 1 | Substrate By similarity | ||||||
| Site | 336 | 1 | Transition state stabilizer By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 204 | 1 | N6-carboxylysine By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 83 – 88 | 6 | YRAKAY → SVQGL in AAA83745. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Characterization of the duplicate ribulose-1,5-bisphosphate carboxylase genes and cbb promoters of Alcaligenes eutrophus." Kusian B., Bednarski R., Husemann M., Bowien B. J. Bacteriol. 177:4442-4450(1995) [PubMed: 7543477] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], EXPRESSION UNDER DIFFERENT GROWTH CONDITIONS. |
| [2] | "Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia eutropha H16." Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I., Gottschalk G., Steinbuechel A., Friedrich B., Bowien B. Nat. Biotechnol. 24:1257-1262(2006) [PubMed: 16964242] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [3] | "Identification of cfxR, an activator gene of autotrophic CO2 fixation in Alcaligenes eutrophus." Windhoevel U., Bowien B. Mol. Microbiol. 5:2695-2705(1991) [PubMed: 1779759] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-10. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | U20584 Genomic DNA. Translation: AAA83745.1. AM260480 Genomic DNA. Translation: CAJ96184.1. M65065 Genomic DNA. Translation: AAA21981.1. |
| PIR | I39557. |
| RefSeq | YP_840914.1. |
3D structure databases | |
| SMR | Q0K1E0. Positions 24-467. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q0K1E0. |
Genome annotation databases | |
| GeneID | 4456354. |
| GenomeReviews | Gene locus H16_B1395 in contig AM260480_GR. |
| KEGG | reh:H16_B1395. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | HBG405441. |
| OMA | QYFAYIA. |
Family and domain databases | |
| HAMAP | MF_01338. RuBisCO_L_type1. [Tree] |
| InterPro | IPR020878. RuBisCo_large_chain_AS. IPR020888. RuBisCO_lsu. IPR000685. RuBisCO_lsu_C. IPR017443. RuBisCO_lsu_fd_N. IPR017444. RuBisCO_lsu_N. [Graphical view] |
| Gene3D | G3DSA:3.20.20.110. RuBisCO_large. 1 hit. G3DSA:3.30.70.150. RuBisCO_large. 1 hit. |
| Pfam | PF00016. RuBisCO_large. 1 hit. PF02788. RuBisCO_large_N. 1 hit. [Graphical view] |
| PROSITE | PS00157. RUBISCO_LARGE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | RBL1C_RALEH | ||||||||
| Accession | Primary (citable) accession number: Q0K1E0 Secondary accession number(s): P09657, P77811 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||

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