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Q0K0X8 (GLGB_CUPNH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:H16_B1559
OrganismCupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha) [Complete proteome] [HAMAP]
Taxonomic identifier381666 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus

Protein attributes

Sequence length756 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7567561,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_1000044994

Sites

Active site4251Nucleophile By similarity
Active site4781Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0K0X8 [UniParc].

Last modified October 3, 2006. Version 1.
Checksum: 0AE35BE8875F370B

FASTA75683,098
        10         20         30         40         50         60 
MTPAHGTQPG MLPGHEFEAL LGARHHDPFA VLGPHPDGDG TLVRACLPGA ASVQLTDAGG 

        70         80         90        100        110        120 
TPLAEMARLH GGGVFAARLP REYKGGAPDY RLRVQWSDGS GQCGADPYAF GLLLGELDLH 

       130        140        150        160        170        180 
LIAEGRHFEL GACLGAQWQR VDGIDGVRFA VWAPNARRVS VIADFNGWHP ARHPMRLRHP 

       190        200        210        220        230        240 
SGIWELFIPA ALGAQPGSRY KYDLLDPHGT ELPDKADPLA LATEAPPATA SVVAGPGQGA 

       250        260        270        280        290        300 
PPFAWHDADW MARRGGADPY AAPMSVYEVH ALSWLRAAND TQRGWEILAE RLVPYVQELG 

       310        320        330        340        350        360 
FTHIELLPIT EHPFGGSWGY QPLSLYAPTA RLGPPQAFAA FIDRCHQAGI GVLLDWVPAH 

       370        380        390        400        410        420 
FPTDPHGLAR FDGTALYEHE DPREGFHQDW NTLIYNLGRN EVRGFLLAGA LHWLEHFHAD 

       430        440        450        460        470        480 
GLRVDAVASM LYRDYSREPG QWVPNRFGGR ENLEAIDFLR ELNAVVHERC PGALTIAEES 

       490        500        510        520        530        540 
TAWPGVTASV ASGGLGFDFK WNMGWMHDTL HYLGHEPVHR AWHHQDMTFG LVYAWSEAFV 

       550        560        570        580        590        600 
LPLSHDEVVH GKASMIGKVP GDEWQRFAGL RAYYGFMWAH PGKKLLFMGG ELAQWQEWNH 

       610        620        630        640        650        660 
DAELDWALLD HPMHRGMHTL VRDLNRLYRE LPALHALDHR PEGFQWVVGD DNHNSVFAWL 

       670        680        690        700        710        720 
RRAGPYSREV VLVVVNMTPV PRYGYRLGVP YAGAWQECLN TDAAVYGGTN VGNSGAVAAV 

       730        740        750 
DVPSHGQPAS LALTLPPLAT LVLRFDPGGG IQGAST 

« Hide

References

[1]"Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia eutropha H16."
Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I., Gottschalk G., Steinbuechel A., Friedrich B., Bowien B.
Nat. Biotechnol. 24:1257-1262(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM260480 Genomic DNA. Translation: CAJ96346.1.
RefSeqYP_841076.1. NC_008314.1.

3D structure databases

ProteinModelPortalQ0K0X8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING381666.H16_B1559.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAJ96346; CAJ96346; H16_B1559.
GeneID4455403.
KEGGreh:H16_B1559.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAHDADWMA.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycCNEC381666:GJUJ-5262-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_CUPNH
AccessionPrimary (citable) accession number: Q0K0X8
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 3, 2006
Last modified: June 11, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries