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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathway:iglycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei425 – 4251NucleophileUniRule annotation
Active sitei478 – 4781Proton donorUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciCNEC381666:GJUJ-5262-MONOMER.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
Ordered Locus Names:H16_B1559
OrganismiCupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
Taxonomic identifieri381666 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
ProteomesiUP000008210 Componenti: Chromosome 2

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7567561,4-alpha-glucan branching enzyme GlgBPRO_1000044994Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi381666.H16_B1559.

Structurei

3D structure databases

ProteinModelPortaliQ0K0X8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiRRYHHDR.
OrthoDBiEOG6JX7GT.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0K0X8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPAHGTQPG MLPGHEFEAL LGARHHDPFA VLGPHPDGDG TLVRACLPGA
60 70 80 90 100
ASVQLTDAGG TPLAEMARLH GGGVFAARLP REYKGGAPDY RLRVQWSDGS
110 120 130 140 150
GQCGADPYAF GLLLGELDLH LIAEGRHFEL GACLGAQWQR VDGIDGVRFA
160 170 180 190 200
VWAPNARRVS VIADFNGWHP ARHPMRLRHP SGIWELFIPA ALGAQPGSRY
210 220 230 240 250
KYDLLDPHGT ELPDKADPLA LATEAPPATA SVVAGPGQGA PPFAWHDADW
260 270 280 290 300
MARRGGADPY AAPMSVYEVH ALSWLRAAND TQRGWEILAE RLVPYVQELG
310 320 330 340 350
FTHIELLPIT EHPFGGSWGY QPLSLYAPTA RLGPPQAFAA FIDRCHQAGI
360 370 380 390 400
GVLLDWVPAH FPTDPHGLAR FDGTALYEHE DPREGFHQDW NTLIYNLGRN
410 420 430 440 450
EVRGFLLAGA LHWLEHFHAD GLRVDAVASM LYRDYSREPG QWVPNRFGGR
460 470 480 490 500
ENLEAIDFLR ELNAVVHERC PGALTIAEES TAWPGVTASV ASGGLGFDFK
510 520 530 540 550
WNMGWMHDTL HYLGHEPVHR AWHHQDMTFG LVYAWSEAFV LPLSHDEVVH
560 570 580 590 600
GKASMIGKVP GDEWQRFAGL RAYYGFMWAH PGKKLLFMGG ELAQWQEWNH
610 620 630 640 650
DAELDWALLD HPMHRGMHTL VRDLNRLYRE LPALHALDHR PEGFQWVVGD
660 670 680 690 700
DNHNSVFAWL RRAGPYSREV VLVVVNMTPV PRYGYRLGVP YAGAWQECLN
710 720 730 740 750
TDAAVYGGTN VGNSGAVAAV DVPSHGQPAS LALTLPPLAT LVLRFDPGGG

IQGAST
Length:756
Mass (Da):83,098
Last modified:October 3, 2006 - v1
Checksum:i0AE35BE8875F370B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM260480 Genomic DNA. Translation: CAJ96346.1.
RefSeqiWP_010814545.1. NC_008314.1.

Genome annotation databases

EnsemblBacteriaiCAJ96346; CAJ96346; H16_B1559.
GeneIDi4455403.
KEGGireh:H16_B1559.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM260480 Genomic DNA. Translation: CAJ96346.1.
RefSeqiWP_010814545.1. NC_008314.1.

3D structure databases

ProteinModelPortaliQ0K0X8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi381666.H16_B1559.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ96346; CAJ96346; H16_B1559.
GeneIDi4455403.
KEGGireh:H16_B1559.

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiRRYHHDR.
OrthoDBiEOG6JX7GT.

Enzyme and pathway databases

UniPathwayiUPA00164.
BioCyciCNEC381666:GJUJ-5262-MONOMER.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

Entry informationi

Entry nameiGLGB_CUPNH
AccessioniPrimary (citable) accession number: Q0K0X8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 3, 2006
Last modified: July 22, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.