Q0K0V6 (METE_CUPNH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	H16_B1581

Organism

Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha) [Complete proteome] [HAMAP]

Taxonomic identifier

381666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	792 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 792	792	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000071615
Sites
	Metal binding	642	1	Zinc By similarity
	Metal binding	644	1	Zinc By similarity
	Metal binding	727	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0K0V6 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: 672ECFBABA3F31E0	FASTA	792	88,039
	10 20 30 40 50 60 MARTHILGFP RIGERRELKF AQEAFWRGDR TEAELRDVAA QLRRRHWQLQ ADRGLDTVAT 70 80 90 100 110 120 GDFAYYDQML SLTALLGALP RRFGLDPARL TLTQYFELAR GNREQPAMEM TKWFDTNYHY 130 140 150 160 170 180 LVPELDAETT FDGGPAWFFE EADEALALGL RARPVLIGPV TYLWLSKSHV AGFDRLALLP 190 200 210 220 230 240 QLLQGYRRIL DQLKARGIEW VQIDEPALCL DLEPAWLDAF DTAYAALREA GPKLLLATYF 250 260 270 280 290 300 DTAADHAQRA AALPVDGFHI DLVRAPEQLA AWQAALPAHA VLSLGVIDGR NIWRTDLRRV 310 320 330 340 350 360 LDTLRPVQAA LGERLWLAPS CSLLHVPVSL AHEVRLDVEL KSWLAFATEK LDELSVLGRA 370 380 390 400 410 420 LNQGDAVVAE ALAASDAAQA SRRASRRVVN PRVQQRLAAV SAGMADRASP FAERIERQRQ 430 440 450 460 470 480 ALQLPLLPTT TIGSFPQTAA IRQTRAAFKR GEIGALEYLE RIRAEIAVAV RKQEALGLDV 490 500 510 520 530 540 LVHGEAERND MVEYFGEQLC GYGFTENGWV QSYGSRCVKP PVIYGDVYRP EPMTVDTARY 550 560 570 580 590 600 AQSLTERPMK GMLTGPITML QWSFVRDDQP RATTARQLAL AIRDEVCDLE QAGIRVIQID 610 620 630 640 650 660 EPALREGLPL RRADWDAYLD WAVTAFRLSA SGVQDQTQIH THMCYAEFND ILPAIAAMDA 670 680 690 700 710 720 DVITIETSRS AMELLEGFGD FDYPNEIGPG VYDIHSPRVP SVQAMERLLD RACEVVPPQR 730 740 750 760 770 780 LWVNPDCGLK TRGWEETEAA LANMVSAARA LRARLSARGA TTWKRLSKPA AATAAAVPHA 790 GNACTACATH AN

References

[1]

"Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia eutropha H16." Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I., Gottschalk G., Steinbuechel A., Friedrich B., Bowien B. Nat. Biotechnol. 24:1257-1262(2006) [PubMed: 16964242] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM260480 Genomic DNA. Translation: CAJ96368.1.
RefSeq	YP_841098.1. NC_008314.1.
3D structure databases
ProteinModelPortal	Q0K0V6.
SMR	Q0K0V6. Positions 3-754.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0K0V6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4457471.
GenomeReviews	Gene locus H16_B1581 in contig AM260480_GR.
KEGG	reh:H16_B1581.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0620.
HOGENOM	HBG287495.
OMA	LWLSKSH.
PhylomeDB	Q0K0V6.
ProtClustDB	PRK05222.
Enzyme and pathway databases
BioCyc	REUT381666:H16_B1581-MONOMER.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_CUPNH

Accession

Primary (citable) accession number: Q0K0V6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	October 3, 2006
Last modified:	November 16, 2011
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program