ID   HGD_RALEH               Reviewed;         439 AA.
AC   Q0K0L8;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2006, sequence version 1.
DT   05-MAY-2009, entry version 18.
DE   RecName: Full=Homogentisate 1,2-dioxygenase;
DE            EC=1.13.11.5;
DE   AltName: Full=Homogentisicase;
DE   AltName: Full=Homogentisate oxygenase;
DE   AltName: Full=Homogentisic acid oxidase;
GN   Name=hmgA; OrderedLocusNames=H16_B1671;
OS   Ralstonia eutropha (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)
OS   (Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier
OS   337)).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Cupriavidus.
OX   NCBI_TaxID=381666;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16964242; DOI=10.1038/nbt1244;
RA   Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H.,
RA   Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E.,
RA   Strittmatter A., Voss I., Gottschalk G., Steinbuechel A.,
RA   Friedrich B., Bowien B.;
RT   "Genome sequence of the bioplastic-producing 'Knallgas' bacterium
RT   Ralstonia eutropha H16.";
RL   Nat. Biotechnol. 24:1257-1262(2006).
CC   -!- CATALYTIC ACTIVITY: Homogentisate + O(2) = 4-maleylacetoacetate.
CC   -!- COFACTOR: Iron (By similarity).
CC   -!- PATHWAY: Amino-acid degradation; L-phenylalanine degradation;
CC       acetoacetic acid and fumarate from L-phenylalanine: step 4/6.
CC   -!- SIMILARITY: Belongs to the homogentisate dioxygenase family.
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DR   EMBL; AM260480; CAJ96456.1; -; Genomic_DNA.
DR   RefSeq; YP_841186.1; -.
DR   GeneID; 4455534; -.
DR   GenomeReviews; AM260480_GR; H16_B1671.
DR   KEGG; reh:H16_B1671; -.
DR   HOGENOM; Q0K0L8; -.
DR   OMA; Q0K0L8; HRNCMSE.
DR   GO; GO:0004411; F:homogentisate 1,2-dioxygenase activity; IEA:HAMAP.
DR   GO; GO:0005506; F:iron ion binding; IEA:HAMAP.
DR   GO; GO:0006559; P:L-phenylalanine catabolic process; IEA:HAMAP.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0006572; P:tyrosine catabolic process; IEA:HAMAP.
DR   HAMAP; MF_00334; -; 1.
DR   InterPro; IPR005708; Homogentis_dOase.
DR   PANTHER; PTHR11056; Homogentis_dOase; 1.
DR   Pfam; PF04209; HgmA; 1.
DR   TIGRFAMs; TIGR01015; hmgA; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Dioxygenase; Iron; Metal-binding; Oxidoreductase;
KW   Phenylalanine catabolism; Tyrosine catabolism.
FT   CHAIN         1    439       Homogentisate 1,2-dioxygenase.
FT                                /FTId=PRO_1000019537.
FT   METAL       336    336       Iron (By similarity).
FT   METAL       342    342       Iron (By similarity).
FT   METAL       372    372       Iron (By similarity).
SQ   SEQUENCE   439 AA;  48708 MW;  29652C9DEC46E3A0 CRC64;
     MTLTHTQLAE HGYMSGFANE FATEALPGAL PVGRNSPQCA PYGLYAEQLS GTAFTAPRAH
     NRRSWLYRIR PAAMHTPFTQ IEQSRFLSRF DQVPPSPNQM RWSPPAMPSV PTDFVDGIVT
     MAGNGGPEAM TGCGIHLYLA NQSMQDRFFY NADGEMLIVP QQGRLLMVTE LGRLEVEPQE
     IVVIPRGVRF RVELPDGEAR GYICENYGAL FQLPDLGVIG SNGLANPRDF LSPVASYEDR
     EGDFELVAKF QGNLWRAGIG HSPLDVVAWH GNYTPYKYDL RRFNTIGSIS FDHPDPSIFL
     VLQSPSATPG VDTIDFVIFG PRWLAMQDSF RPPWFHRNIA SEFMGLITGV YDAKAEGFAP
     GGASLHNCMS GHGPDAETFE KASAADTSKP HRIEDTMAFM FETPGVIRPT PYAAQSASLQ
     QDYYTCWQGL KKHFNPNTR
//