Reviewed, UniProtKB/Swiss-Prot Q0K0L8 (HGD_RALEH)

Names and origin

Protein names

Recommended name:
    Homogentisate 1,2-dioxygenase
    EC=1.13.11.5
Alternative name(s):
    Homogentisicase
    Homogentisate oxygenase
    Homogentisic acid oxidase

Gene names

Name:	hmgA
Ordered Locus Names:	H16_B1671

Organism

Ralstonia eutropha (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)) [Complete proteome] [HAMAP]

Taxonomic identifier

381666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	439 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Homogentisate + O2 = 4-maleylacetoacetate. HAMAP MF_00334
Cofactor	Iron By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 4/6. HAMAP MF_00334
Sequence similarities	Belongs to the homogentisate dioxygenase family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tyrosine catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	homogentisate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 439	439	Homogentisate 1,2-dioxygenase HAMAP MF_00334		PRO_1000019537
Sites
	Metal binding	336	1	Iron By similarity
	Metal binding	342	1	Iron By similarity
	Metal binding	372	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0K0L8-1 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: 29652C9DEC46E3A0	FASTA	439	48,708
	10 20 30 40 50 60 MTLTHTQLAE HGYMSGFANE FATEALPGAL PVGRNSPQCA PYGLYAEQLS GTAFTAPRAH 70 80 90 100 110 120 NRRSWLYRIR PAAMHTPFTQ IEQSRFLSRF DQVPPSPNQM RWSPPAMPSV PTDFVDGIVT 130 140 150 160 170 180 MAGNGGPEAM TGCGIHLYLA NQSMQDRFFY NADGEMLIVP QQGRLLMVTE LGRLEVEPQE 190 200 210 220 230 240 IVVIPRGVRF RVELPDGEAR GYICENYGAL FQLPDLGVIG SNGLANPRDF LSPVASYEDR 250 260 270 280 290 300 EGDFELVAKF QGNLWRAGIG HSPLDVVAWH GNYTPYKYDL RRFNTIGSIS FDHPDPSIFL 310 320 330 340 350 360 VLQSPSATPG VDTIDFVIFG PRWLAMQDSF RPPWFHRNIA SEFMGLITGV YDAKAEGFAP 370 380 390 400 410 420 GGASLHNCMS GHGPDAETFE KASAADTSKP HRIEDTMAFM FETPGVIRPT PYAAQSASLQ 430 QDYYTCWQGL KKHFNPNTR

References

[1]

"Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia eutropha H16." Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I., Gottschalk G., Steinbuechel A., Friedrich B., Bowien B. Nat. Biotechnol. 24:1257-1262(2006) [PubMed: 16964242] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM260480 Genomic DNA. Translation: CAJ96456.1.
RefSeq	YP_841186.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q0K0L8.
Genome annotation databases
GeneID	4455534.
GenomeReviews	Gene locus H16_B1671 in contig AM260480_GR.
KEGG	reh:H16_B1671.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0K0L8.
OMA	HRNCMSE.
Family and domain databases
HAMAP	MF_00334. [Tree]
InterPro	IPR005708. Homogentis_dOase. [Graphical view]
PANTHER	PTHR11056. Homogentis_dOase. 1 hit.
Pfam	PF04209. HgmA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01015. hmgA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HGD_RALEH

Accession

Primary (citable) accession number: Q0K0L8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 3, 2006
Last modified:	November 3, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)