Q0K0L8 (HGD_CUPNH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homogentisate 1,2-dioxygenase
EC=1.13.11.5

Alternative name(s):
Homogentisate oxygenase
Homogentisic acid oxidase
Homogentisicase

Gene names

Name:	hmgA
Ordered Locus Names:	H16_B1671

Organism

Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha) [Complete proteome] [HAMAP]

Taxonomic identifier

381666 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus › Cupriavidus necator

Protein attributes

Sequence length	439 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Homogentisate + O2 = 4-maleylacetoacetate. HAMAP-Rule MF_00334
Cofactor	Iron By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 4/6. HAMAP-Rule MF_00334
Sequence similarities	Belongs to the homogentisate dioxygenase family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: HAMAP tyrosine catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	homogentisate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 439	439	Homogentisate 1,2-dioxygenase HAMAP-Rule MF_00334		PRO_1000019537
Sites
	Metal binding	336	1	Iron By similarity
	Metal binding	342	1	Iron By similarity
	Metal binding	372	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0K0L8 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: 29652C9DEC46E3A0	FASTA	439	48,708
	10 20 30 40 50 60 MTLTHTQLAE HGYMSGFANE FATEALPGAL PVGRNSPQCA PYGLYAEQLS GTAFTAPRAH 70 80 90 100 110 120 NRRSWLYRIR PAAMHTPFTQ IEQSRFLSRF DQVPPSPNQM RWSPPAMPSV PTDFVDGIVT 130 140 150 160 170 180 MAGNGGPEAM TGCGIHLYLA NQSMQDRFFY NADGEMLIVP QQGRLLMVTE LGRLEVEPQE 190 200 210 220 230 240 IVVIPRGVRF RVELPDGEAR GYICENYGAL FQLPDLGVIG SNGLANPRDF LSPVASYEDR 250 260 270 280 290 300 EGDFELVAKF QGNLWRAGIG HSPLDVVAWH GNYTPYKYDL RRFNTIGSIS FDHPDPSIFL 310 320 330 340 350 360 VLQSPSATPG VDTIDFVIFG PRWLAMQDSF RPPWFHRNIA SEFMGLITGV YDAKAEGFAP 370 380 390 400 410 420 GGASLHNCMS GHGPDAETFE KASAADTSKP HRIEDTMAFM FETPGVIRPT PYAAQSASLQ 430 QDYYTCWQGL KKHFNPNTR

References

[1]

"Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia eutropha H16." Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I., Gottschalk G., Steinbuechel A., Friedrich B., Bowien B. Nat. Biotechnol. 24:1257-1262(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM260480 Genomic DNA. Translation: CAJ96456.1.
RefSeq	YP_841186.1. NC_008314.1.
3D structure databases
ProteinModelPortal	Q0K0L8.
SMR	Q0K0L8. Positions 9-439.
ModBase	Search...
Protein-protein interaction databases
STRING	381666.H16_B1671.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAJ96456; CAJ96456; H16_B1671.
GeneID	4455534.
KEGG	reh:H16_B1671.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3508.
HOGENOM	HOG000139824.
KO	K00451.
OMA	AVTKWGL.
ProtClustDB	PRK05341.
Enzyme and pathway databases
BioCyc	CNEC381666:GJUJ-5374-MONOMER.
UniPathway	UPA00139; UER00339.
Family and domain databases
Gene3D	2.60.120.10. 2 hits.
HAMAP	MF_00334. Homogentis_dioxygen.
InterPro	IPR005708. Homogentis_dOase. IPR022950. Homogentis_dOase_bac. IPR014710. RmlC-like_jellyroll. IPR011051. RmlC_Cupin. [Graphical view]
PANTHER	PTHR11056. PTHR11056. 1 hit.
Pfam	PF04209. HgmA. 1 hit. [Graphical view]
SUPFAM	SSF51182. RmlC_like_cupin. 1 hit.
TIGRFAMs	TIGR01015. hmgA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HGD_CUPNH

Accession

Primary (citable) accession number: Q0K0L8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 3, 2006
Last modified:	May 1, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program