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Q0JZ73 (Q0JZ73_CUPNH) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP-Rule MF_00191
EC=1.17.1.2 HAMAP-Rule MF_00191
Gene names
Name:ispH HAMAP-Rule MF_00191
Ordered Locus Names:H16_B2169 EMBL CAJ96951.1, h16_B2169 EMBL CAJ96951.1
OrganismCupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha) [Complete proteome] [HAMAP] EMBL CAJ96951.1
Taxonomic identifier381666 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus ›

Protein attributes

Sequence length337 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. SAAS SAAS003451 HAMAP-Rule MF_00191

Catalytic activity

Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. SAAS SAAS003451 HAMAP-Rule MF_00191

Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. SAAS SAAS003451 HAMAP-Rule MF_00191

Cofactor

Binds 1 3Fe-4S cluster per subunit By similarity. HAMAP-Rule MF_00191 SAAS SAAS003451

Pathway

Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. SAAS SAAS003451 HAMAP-Rule MF_00191

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. SAAS SAAS003451 HAMAP-Rule MF_00191

Sequence similarities

Belongs to the IspH family. HAMAP-Rule MF_00191

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Region222 – 2243Substrate binding By similarity HAMAP-Rule MF_00191

Sites

Metal binding121Iron-sulfur (3Fe-4S) By similarity HAMAP-Rule MF_00191
Metal binding961Iron-sulfur (3Fe-4S) By similarity HAMAP-Rule MF_00191
Metal binding1941Iron-sulfur (3Fe-4S) By similarity HAMAP-Rule MF_00191
Binding site411Substrate By similarity HAMAP-Rule MF_00191
Binding site741Substrate By similarity HAMAP-Rule MF_00191
Binding site1241Substrate By similarity HAMAP-Rule MF_00191
Binding site1641Substrate By similarity HAMAP-Rule MF_00191
Binding site2661Substrate By similarity HAMAP-Rule MF_00191

Sequences

Sequence LengthMass (Da)Tools
Q0JZ73 [UniParc].

Last modified October 3, 2006. Version 1.
Checksum: F44641947CE9A93A

FASTA33735,825
        10         20         30         40         50         60 
MQVILAQPRG FCAGVVRAIE IVDRALVKHG APVFVRHEIV HNKHVVEGLK DKGARFVEEL 

        70         80         90        100        110        120 
DEVPTGAVTI FSAHGVSREV IADASQRQLH AIDATCPLVI KVHTQGRQYA ASGRTVILIG 

       130        140        150        160        170        180 
HAGHPEVEGT MGQIPGKVIL VQNEAEVAQL DLPADTPVAY VTQTTLSVDD TRNIIAALGR 

       190        200        210        220        230        240 
RFSNLVGPDT RDICYATQNR QSAVRDLCKL ADVILVIGAT NSSNSNRLRE IGTESGVPSY 

       250        260        270        280        290        300 
LIAEGAELDP AWVRDANVVG ITAGASAPEE MVEDVINALR RLGPVDVTTM AGREEHAEFR 

       310        320        330 
LPPELADVKP QSAPRKAGKE PAAVAAAPAT ANENIAG

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References

[1]"Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia eutropha H16."
Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I., Gottschalk G., Steinbuechel A., Friedrich B., Bowien B.
Nat. Biotechnol. 24:1257-1262(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AM260480 Genomic DNA. Translation: CAJ96951.1.
RefSeqYP_841681.1. NC_008314.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRING381666.H16_B2169.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAJ96951; CAJ96951; H16_B2169.
GeneID4456039.
KEGGreh:H16_B2169.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0761.
HOGENOMHOG000220192.
KOK03527.
OMAEENIEFR.
ProtClustDBPRK01045.

Enzyme and pathway databases

BioCycCNEC381666:GJUJ-5869-MONOMER.
UniPathwayUPA00056; UER00097.
UPA00059; UER00105.

Family and domain databases

HAMAPMF_00191. IspH.
InterProIPR003451. LytB.
[Graphical view]
PfamPF02401. LYTB. 1 hit.
[Graphical view]
TIGRFAMsTIGR00216. ispH_lytB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameQ0JZ73_CUPNH
AccessionPrimary (citable) accession number: Q0JZ73
Entry history
Integrated into UniProtKB/TrEMBL: October 3, 2006
Last sequence update: October 3, 2006
Last modified: May 1, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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