Q0JR25 (IBBR_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bowman-Birk type bran trypsin inhibitor

Alternative name(s):
OSE727A
Protein RBBI3-3
RBTI

Gene names

Name:	RBBI3.3
Ordered Locus Names:	Os01g0124401, Os01g0124400, LOC_Os01g03360
ORF Names:	P0037C04.19, P0044F08.1

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities	Belongs to the Bowman-Birk serine protease inhibitor family.
Sequence caution	The sequence AAB68026.1 differs from that shown. Reason: Erroneous initiation. The sequence AAD10378.1 differs from that shown. Reason: Chimeric cDNA. Its N-terminal part is derived from the gene LOC_Os01g03390, which encoded for an RBBI2.3 protein. The sequence BAD52869.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Domain	Repeat Signal
Molecular function	Protease inhibitor Serine protease inhibitor
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: InterPro
Molecular function	serine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 22

22

Potential

Propeptide

23 – 118

96

PRO_0000003286

Chain

119 – 251

133

Bowman-Birk type bran trypsin inhibitor

PRO_0000003287

Propeptide

252 – 254

3

PRO_0000003288

Regions

Repeat

46 – 120

75

Repeat

121 – 187

67

Repeat

188 – 251

64

Sites

Site

135 – 136

2

Reactive bond for trypsin By similarity

Site

201 – 202

2

Reactive bond for trypsin By similarity

Amino acid modifications

Disulfide bond

125 ↔ 185

Ref.7

Disulfide bond

126 ↔ 143

Ref.7

Disulfide bond

152 ↔ 159

Ref.7

Disulfide bond

156 ↔ 172

Ref.7

Disulfide bond

193 ↔ 248

Ref.7

Disulfide bond

194 ↔ 209

Ref.7

Disulfide bond

199 ↔ 207

Ref.7

Disulfide bond

216 ↔ 223

Ref.7

Disulfide bond

220 ↔ 236

Ref.7

Experimental info

Sequence conflict

121

1

R → K in AAB68026. Ref.5

Sequence conflict

121

1

R → K in AAD10378. Ref.5

Sequence conflict

130

1

K → E in AAB68026. Ref.5

Sequence conflict

130

1

K → E in AAD10378. Ref.5

Sequence conflict

136 – 137

2

PD → TN in AAB68026. Ref.5

Sequence conflict

136 – 137

2

PD → TN in AAD10378. Ref.5

Sequence conflict

189

1

P → T in AAB68026. Ref.5

Sequence conflict

189

1

P → T in AAD10378. Ref.5

Secondary structure

1

.

.

.

.

.

.

.

.

.

254

Helix Strand Turn

Details...

Beta strand

127 – 131

5

Beta strand

141 – 143

3

Beta strand

172 – 176

5

Turn

203 – 205

3

Sequences

	Sequence		Length	Mass (Da)
	Q0JR25 [UniParc]. Last modified July 10, 2007. Version 2. Checksum: 32630D7DE579C1E8	FASTA	254	27,790
	10 20 30 40 50 60 MSNTTMATST ILLFLLAGLA AAHGDGDTTI RLPSDGAKAS RPRAAKPWDC CDNIEISRLM 70 80 90 100 110 120 IYPPLYRCND EVKQCAAACK ECVEAPGGDF NGGAFVCSDW FSTVDPGPKC TAALDGLSME 130 140 150 160 170 180 RPWKCCDNIK RLPTKPDPPQ WRCNDELEPS QCTAACKSCR EAPGPFPGKL ICEDIYWGAD 190 200 210 220 230 240 PGPFCTPRPW GDCCDKAFCN KMNPPTCRCM DEVKECADAC KDCQRVESSE PPRYVCKDRF 250 TGHPGPVCKP RAEN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, expression and localization pattern of a trypsin inhibitor gene from rice." Masumura T., Fujioka M., Matsui Y., Kumazawa Y., Tashiro M., Morita S., Tanaka K. Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nipponbare. Tissue: Embryo.
[2]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed: 18089549] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[5]	"Nucleotide sequence of a cDNA encoding rice Bowman-Birk proteinase inhibitor." Chen P.-W., Chow S.-H., Chen L.-J. Plant Gene Register PGR97-015 Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-254. Strain: cv. Lomello. Tissue: Embryo.
[6]	"The complete amino acid sequence of rice bran trypsin inhibitor." Tashiro M., Hashino K., Shiozaki M., Ibuki F., Maki Z. J. Biochem. 102:297-306(1987) [PubMed: 3667571] [Abstract] Cited for: PROTEIN SEQUENCE OF 119-251. Strain: cv. Nipponbare.
[7]	"Purification, crystallization and preliminary X-ray crystallographic analysis of rice Bowman-Birk inhibitor from Oryza sativa." Lin Y.H., Li H.T., Huang Y.C., Hsieh Y.C., Guan H.H., Liu M.Y., Chang T., Wang A.H., Chen C.J. Acta Crystallogr. F 62:522-524(2006) [PubMed: 16754971] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 119-251, DISULFIDE BONDS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB098712 mRNA. Translation: BAC78439.1.
AP002909 Genomic DNA. Translation: BAB21173.1.
AP003233 Genomic DNA. Translation: BAD52869.1. Sequence problems.
AP008207 Genomic DNA. Translation: BAH90883.1.
U57640 mRNA. Translation: AAD10378.1. Sequence problems.
U76004 mRNA. Translation: AAB68026.1. Different initiation.

PIR

TIRZBR. A27193.
T04161.

RefSeq

NP_001172153.1. NM_001185224.1.

UniGene

Os.74608.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2QN5	X-ray	3.00	B	119-251	[»]

ProteinModelPortal

Q0JR25.

ModBase

Search...

Protein-protein interaction databases

STRING

Q0JR25.

Protein family/group databases

MEROPS

I12.007.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblPlants

LOC_Os01g03360.1; LOC_Os01g03360.1; LOC_Os01g03360.

GeneID

9271230.

KEGG

osa:9271230.

NMPDR

fig|39947.1.peg.1022.

Organism-specific databases

Gramene

P07084.

Phylogenomic databases

GeneTree

EPGT00050000011034.

Family and domain databases

InterPro

IPR000877. Prot_inh_BBI.
[Graphical view]

Gene3D

G3DSA:2.10.69.10. Prot_inh_BBI. 3 hits.

Pfam

PF00228. Bowman-Birk_leg. 4 hits.
[Graphical view]

SMART

SM00269. BowB. 3 hits.
[Graphical view]

SUPFAM

SSF57247. Prot_inh_BBI. 3 hits.

PROSITE

PS00281. BOWMAN_BIRK. 2 hits.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

IBBR_ORYSJ

Accession

Primary (citable) accession number: Q0JR25
Secondary accession number(s): C7IWR7 , P07084, P93432, Q5ZCA7, Q7XZC9, Q9AWV9, Q9M3W2, Q9SAS2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	July 10, 2007
Last modified:	September 21, 2011
This is version 37 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program