Reviewed, UniProtKB/Swiss-Prot Q0JPT5 (NIP12_ORYSJ)

Names and origin

Protein names

Recommended name:
    Aquaporin NIP1-2
Alternative name(s):
    NOD26-like intrinsic protein 1-2
    OsNIP1;2

Gene names

Name:	NIP1-2
Ordered Locus Names:	Os01g0202800/Os01g0202900, LOC_Os01g10600
ORF Names:	P0489A05.30, OsJ_000763

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Aquaporins facilitate the transport of water and small neutral solutes across cell membranes By similarity.
Subcellular location	Membrane; Multi-pass membrane protein Probable.
Tissue specificity	Expressed in roots and leaves, and at lower levels in anthers. Ref.5
Domain	Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Sequence similarities	Belongs to the MIP/aquaporin (TC 1.A.8) family. NIP subfamily. [View classification]
Sequence caution	The sequence BAF04242.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes Os01g0202800 and Os01g0202900. The sequence BAF04243.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes Os01g0202800 and Os01g0202900.

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane
Domain	Repeat Transmembrane
Gene Ontology (GO)
Biological process	transmembrane transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	Aquaporin NIP1-2		PRO_0000286027
Regions
	Transmembrane	66 – 86	21	1 Potential
	Transmembrane	91 – 111	21	2 Potential
	Transmembrane	145 – 165	21	3 Potential
	Transmembrane	188 – 208	21	4 Potential
	Transmembrane	212 – 232	21	5 Potential
	Transmembrane	255 – 275	21	6 Potential
	Motif	123 – 125	3	NPA 1
	Motif	241 – 243	3	NPA 2
	Compositional bias	31 – 37	7	Poly-Ser
	Compositional bias	164 – 169	6	Poly-Gly

Sequences

	Sequence		Length	Mass (Da)
	Q0JPT5-1 [UniParc]. Last modified May 1, 2007. Version 2. Checksum: E2AFCAB79D1BEE77	FASTA	303	31,534
	10 20 30 40 50 60 MAGREDGAAA GAMEEGQDSK EVKCESSEDG SSSSSSSRCH GNDVISVQFM QKVHPWCMCM 70 80 90 100 110 120 NKNLLILAEI LGTYFMIFAG CGAVVVNQST GGAVTFPGIC AVWGLVVMVL VYTVSHISGA 130 140 150 160 170 180 HFNPAVTVAF ATCGRFRWKQ VPSYVVAQVL GSTMASLTLR VVFGGGGGGA RGEHLFFGTT 190 200 210 220 230 240 PAGSMAQAAA LEFVISFFLM FVVSGVATDN RAIGELAGLA VGATVAVNVL FAGPVTGASM 250 260 270 280 290 300 NPARSLGPAM VAGRYGGVWV YVAAPVSGTV CGAWAYNLLR FTDKPLRDIA NTASFLRRSS RRS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[5]	"Identification of 33 rice aquaporin genes and analysis of their expression and function." Sakurai J., Ishikawa F., Yamaguchi T., Uemura M., Maeshima M. Plant Cell Physiol. 46:1568-1577(2005) [PubMed: 16033806] [Abstract] Cited for: NOMENCLATURE, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP003105 Genomic DNA. Translation: BAD73177.1. AP008207 Genomic DNA. Translation: BAF04242.1. Sequence problems. AP008207 Genomic DNA. Translation: BAF04243.1. Sequence problems. CM000138 Genomic DNA. Translation: EAZ10938.1.
RefSeq	NP_001042328.1. NP_001042329.1.
UniGene	Os.80672 Os.80682
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4327074. 4327075.
GenomeReviews	Gene locus NIP1-2 in contig AP008207_GR.
KEGG	osa:4327074. osa:4327075.
NMPDR	fig|39947.1.peg.5273.
Organism-specific databases
Gramene	Q5QN31.
Phylogenomic databases
HOGENOM	HBG705794.
Family and domain databases
InterPro	IPR012269. Aquaporin. IPR000425. MIP. [Graphical view]
Gene3D	G3DSA:1.20.1080.10. MIP. 1 hit.
PANTHER	PTHR19139. MIP. 1 hit.
Pfam	PF00230. MIP. 1 hit. [Graphical view]
PRINTS	PR00783. MINTRINSICP.
PROSITE	PS00221. MIP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NIP12_ORYSJ

Accession

Primary (citable) accession number: Q0JPT5
Secondary accession number(s): Q0JPT6, Q5QN31

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	May 1, 2007
Last modified:	December 15, 2009
This is version 25 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)