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Protein

Thiamine pyrophosphokinase 2

Gene

TPK2

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate (TPP). TPP is an active cofactor for enzymes involved in glycolysis and energy production. Plant leaves require high levels of TPP for photosynthesis and carbohydrate metabolism (By similarity).By similarity

Catalytic activityi

ATP + thiamine = AMP + thiamine diphosphate.

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes thiamine diphosphate from thiamine.
Proteins known to be involved in this subpathway in this organism are:
  1. Thiamine pyrophosphokinase 1 (TPK1), Thiamine pyrophosphokinase 2 (TPK2), Thiamine pyrophosphokinase (OsJ_01724), Thiamine pyrophosphokinase 3 (TPK3)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes thiamine diphosphate from thiamine, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-OSA-196819. Vitamin B1 (thiamin) metabolism.
UniPathwayiUPA00060; UER00597.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine pyrophosphokinase 2 (EC:2.7.6.2)
Short name:
OsTPK2
Alternative name(s):
Thiamine kinase 2
Gene namesi
Name:TPK2
Ordered Locus Names:Os01g0356500, LOC_Os01g25440
ORF Names:B1157F09.24, P0025H06.10
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004239691 – 267Thiamine pyrophosphokinase 2Add BLAST267

Proteomic databases

PaxDbiQ0JMW0.

Expressioni

Gene expression databases

GenevisibleiQ0JMW0. OS.

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os01g25440.1.

Structurei

3D structure databases

SMRiQ0JMW0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thiamine pyrophosphokinase family.Curated

Phylogenomic databases

eggNOGiKOG3153. Eukaryota.
COG1564. LUCA.
InParanoidiQ0JMW0.
KOiK00949.
OMAiESKMRFG.
OrthoDBiEOG09360JCF.

Family and domain databases

CDDicd07995. TPK. 1 hit.
Gene3Di2.60.120.320. 1 hit.
3.40.50.10240. 1 hit.
InterProiView protein in InterPro
IPR006282. Thi_PPkinase.
IPR016966. Thiamin_pyrophosphokinase_euk.
IPR007373. Thiamin_PyroPKinase_B1-bd.
IPR036371. TPK_B1-bd_sf.
IPR007371. TPK_catalytic.
IPR036759. TPK_catalytic_sf.
PfamiView protein in Pfam
PF04265. TPK_B1_binding. 1 hit.
PF04263. TPK_catalytic. 1 hit.
PIRSFiPIRSF031057. Thiamin_pyrophosphokinase. 1 hit.
SMARTiView protein in SMART
SM00983. TPK_B1_binding. 1 hit.
SUPFAMiSSF63862. SSF63862. 1 hit.
SSF63999. SSF63999. 1 hit.
TIGRFAMsiTIGR01378. thi_PPkinase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0JMW0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGRAIRSME VMVHSSTFLL PKLHQPANSP AKKYALVVLN QNLPRFVPRL
60 70 80 90 100
WTHAKLRICA DGGANRIFDE MFQMTNDPDY ESTRKRYIPE IIEGDMDSIR
110 120 130 140 150
PEVKQFYSSQ GSKISDKSHN QETTDLHKCI SRIHRCTPDH EKTNLCVLVT
160 170 180 190 200
GALGGRFDHE AANINILYLF SDMRIVLLSD DCLIRLLPKT HKHEIYIESS
210 220 230 240 250
VEGPHCGLFP VGAPSGSTTT TGLKWNLSEA KMRFGSMIST SNIVHAEKVT
260
VQSDADLLWT ISLRNLT
Length:267
Mass (Da):30,107
Last modified:October 3, 2006 - v1
Checksum:iA21BDF30C4C59073
GO

Sequence cautioni

Q0JMW0: The sequence BAD52763 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
Q0JMW0: The sequence BAD53067 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP003207 Genomic DNA. Translation: BAD52763.1. Different initiation.
AP003312 Genomic DNA. Translation: BAD53067.1. Different initiation.
AP008207 Genomic DNA. Translation: BAF04918.1.
AP014957 Genomic DNA. Translation: BAS72068.1.
AK070843 mRNA. Translation: BAG92167.1.
RefSeqiXP_015621862.1. XM_015766376.1.
UniGeneiOs.64684.

Genome annotation databases

EnsemblPlantsiOS01T0356500-01; OS01T0356500-01; OS01G0356500.
GeneIDi4326288.
GrameneiOS01T0356500-01; OS01T0356500-01; OS01G0356500.
KEGGiosa:4326288.

Similar proteinsi

Entry informationi

Entry nameiTPK2_ORYSJ
AccessioniPrimary (citable) accession number: Q0JMW0
Secondary accession number(s): A0A0P0V2C5, Q5ZBJ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 16, 2013
Last sequence update: October 3, 2006
Last modified: October 25, 2017
This is version 71 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families