Q0JMV4 (AMYC1_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-amylase isozyme C
EC=3.2.1.1

Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Alpha-amylase isozyme 1B

Gene names

Name:	AMY1B
Synonyms:	AMYC
Ordered Locus Names:	Os01g0357400, LOC_Os01g25510
ORF Names:	P0025H06.22, P0514H03.1

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Important for breakdown of endosperm starch during germination.
Catalytic activity	Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.
Cofactor	Binds 3 calcium ions per subunit By similarity.
Subunit structure	Monomer By similarity.
Tissue specificity	In callus, weakly expressed. Ref.1
Developmental stage	Expressed at a high level during germination in the aleurones cells under the control of the plant hormone gibberellic acid and in the developing grains at a low level. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.
Sequence caution	The sequence AAA33893.1 differs from that shown. Reason: Frameshift at several positions. The sequence AAA33893.1 differs from that shown. Reason: Sequencing errors. The sequence BAD52958.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAD53075.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Ligand	Calcium Metal-binding
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-amylase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 348	348	Alpha-amylase isozyme C		PRO_0000054294
Regions
	Region	87 – 88	2	Substrate binding By similarity
	Region	182 – 187	6	Substrate binding By similarity
	Region	289 – 291	3	Substrate binding By similarity
Sites
	Metal binding	116	1	Calcium By similarity
	Metal binding	188	1	Calcium; via carbonyl oxygen By similarity
	Binding site	214	1	Substrate By similarity
	Binding site	283	1	Substrate By similarity
	Binding site	302	1	Substrate By similarity
	Binding site	344	1	Substrate By similarity
	Site	303	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0JMV4 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: 63D1914555DCE02B	FASTA	348	38,478
	10 20 30 40 50 60 MQVPIEHNGG EQTLLVPFRA HRPPRPLLQL GSRASPVSGF QLGVVEGEWR VVQPAYGQAR 70 80 90 100 110 120 WTTSPPPASP TSGSLRRPTL SASKATCWGG CTIWTRPIIE AFHGKGVQVI ADIVINHRTA 130 140 150 160 170 180 EHKDSRGIYC RLPPRLGPAH DLPRRPLRRR HRKPGHRRRT STTSTSASSG SSSAGSTGSR 190 200 210 220 230 240 WTSASTRGAS TSPRATPPTW QRSTSMPPSR ASPWPRYGRR WRTAGTASRT TTRTRTGRSW 250 260 270 280 290 300 STGSIVSAAP TAMPRRSTSP PRASSTSPWR AIELWRLRGE DGKAPGMIGW WPAKATTFVD 310 320 330 340 NHDTGNPCIF YDHFFDWGLK DEIERLVSIR NRQGIHPARG RCCWLLPS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural organization and differential expression of rice alpha-amylase genes." Huang N., Koizumi N., Reinl S.J., Rodriguez R.L. Nucleic Acids Res. 18:7007-7014(1990) [PubMed: 2263460] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: cv. M202. Tissue: Etiolated leaf.
[2]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed: 18089549] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[5]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M59350 Genomic DNA. Translation: AAA33893.1. Sequence problems. AP003275 Genomic DNA. Translation: BAD52958.1. Sequence problems. AP003312 Genomic DNA. Translation: BAD53075.1. Sequence problems. AP008207 Genomic DNA. Translation: BAF04924.1. AK063489 mRNA. No translation available.
RefSeq	NP_001043010.1. NM_001049545.1.
UniGene	Os.79285.
3D structure databases
ProteinModelPortal	Q0JMV4.
SMR	Q0JMV4. Positions 96-130, 273-336.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4326017.
KEGG	osa:4326017.
Organism-specific databases
Gramene	P27940.
Phylogenomic databases
PhylomeDB	Q0JMV4.
Family and domain databases
InterPro	IPR015902. Alpha_amylase. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR10357. Alpha_amylase. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AMYC1_ORYSJ

Accession

Primary (citable) accession number: Q0JMV4
Secondary accession number(s): P27940, Q5ZBK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 12, 2007
Last sequence update:	October 3, 2006
Last modified:	December 14, 2011
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program