ID   P2C06_ORYSJ             Reviewed;         467 AA.
AC   Q0JLP9; A0A0P0V4J5;
DT   10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2006, sequence version 1.
DT   24-JAN-2024, entry version 108.
DE   RecName: Full=Probable protein phosphatase 2C 6 {ECO:0000305};
DE            Short=OsPP2C06 {ECO:0000303|PubMed:19021904};
DE            EC=3.1.3.16 {ECO:0000305};
DE   AltName: Full=ABI1-like protein 1 {ECO:0000305};
DE            Short=OsABI-LIKE1 {ECO:0000303|PubMed:26491145};
DE            Short=OsABIL1 {ECO:0000303|PubMed:26491145};
GN   Name=PP2C06 {ECO:0000303|PubMed:19021904};
GN   Synonyms=ABIL1 {ECO:0000303|PubMed:26491145};
GN   OrderedLocusNames=Os01g0583100 {ECO:0000312|EMBL:BAS72874.1},
GN   LOC_Os01g40094 {ECO:0000305};
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 72-467.
RC   STRAIN=cv. Nipponbare;
RG   The rice full-length cDNA consortium;
RT   "Oryza sativa full length cDNA.";
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=19021904; DOI=10.1186/1471-2164-9-550;
RA   Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.;
RT   "Genome-wide and expression analysis of protein phosphatase 2C in rice and
RT   Arabidopsis.";
RL   BMC Genomics 9:550-550(2008).
RN   [6]
RP   SUBCELLULAR LOCATION, AND INDUCTION BY ABSCISIC ACID.
RX   PubMed=26491145; DOI=10.1093/pcp/pcv154;
RA   Li C., Shen H., Wang T., Wang X.;
RT   "ABA regulates subcellular redistribution of OsABI-LIKE2, a negative
RT   regulator in ABA signaling, to control root architecture and drought
RT   resistance in Oryza sativa.";
RL   Plant Cell Physiol. 56:2396-2408(2015).
RN   [7]
RP   X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS), AND INTERACTION WITH PYL9.
RX   PubMed=24743650; DOI=10.1371/journal.pone.0095246;
RA   He Y., Hao Q., Li W., Yan C., Yan N., Yin P.;
RT   "Identification and characterization of ABA receptors in Oryza sativa.";
RL   PLoS ONE 9:E95246-E95246(2014).
CC   -!- FUNCTION: Probable protein phosphatase that may function in abscisic
CC       acid (ABA) signaling. {ECO:0000250|UniProtKB:Q6L4R7}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + O-phospho-L-seryl-[protein] = L-seryl-[protein] +
CC         phosphate; Xref=Rhea:RHEA:20629, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC         COMP:11604, ChEBI:CHEBI:15377, ChEBI:CHEBI:29999, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:83421; EC=3.1.3.16; Evidence={ECO:0000305};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + O-phospho-L-threonyl-[protein] = L-threonyl-[protein] +
CC         phosphate; Xref=Rhea:RHEA:47004, Rhea:RHEA-COMP:11060, Rhea:RHEA-
CC         COMP:11605, ChEBI:CHEBI:15377, ChEBI:CHEBI:30013, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:61977; EC=3.1.3.16; Evidence={ECO:0000305};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC       Note=Binds 2 magnesium or manganese ions per subunit. {ECO:0000250};
CC   -!- SUBUNIT: Interacts with PYL9. {ECO:0000269|PubMed:24743650}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:26491145}. Cytoplasm,
CC       cytosol {ECO:0000269|PubMed:26491145}. Note=Localizes predominantly in
CC       nucleus. {ECO:0000269|PubMed:26491145}.
CC   -!- INDUCTION: Induced by abscisic acid (ABA).
CC       {ECO:0000269|PubMed:26491145}.
CC   -!- MISCELLANEOUS: Plants overexpressing PP2C06 exhibit dramatically
CC       reduced fertility and severe pre-harvest sprouting.
CC       {ECO:0000269|PubMed:26491145}.
CC   -!- SIMILARITY: Belongs to the PP2C family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAF05329.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=BAS72874.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AP008207; BAF05329.2; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP014957; BAS72874.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AK242616; -; NOT_ANNOTATED_CDS; mRNA.
DR   RefSeq; XP_015621667.1; XM_015766181.1.
DR   PDB; 4OIC; X-ray; 2.00 A; B=1-467.
DR   PDBsum; 4OIC; -.
DR   AlphaFoldDB; Q0JLP9; -.
DR   SMR; Q0JLP9; -.
DR   BioGRID; 793238; 1.
DR   STRING; 39947.Q0JLP9; -.
DR   PaxDb; 39947-Q0JLP9; -.
DR   GeneID; 4324201; -.
DR   KEGG; osa:4324201; -.
DR   eggNOG; KOG0698; Eukaryota.
DR   HOGENOM; CLU_013173_20_4_1; -.
DR   InParanoid; Q0JLP9; -.
DR   OrthoDB; 216736at2759; -.
DR   Proteomes; UP000000763; Chromosome 1.
DR   Proteomes; UP000059680; Chromosome 1.
DR   GO; GO:0005829; C:cytosol; IDA:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0017018; F:myosin phosphatase activity; IEA:UniProtKB-EC.
DR   GO; GO:0004722; F:protein serine/threonine phosphatase activity; IBA:GO_Central.
DR   GO; GO:0009738; P:abscisic acid-activated signaling pathway; IEA:UniProtKB-KW.
DR   CDD; cd00143; PP2Cc; 1.
DR   Gene3D; 3.60.40.10; PPM-type phosphatase domain; 1.
DR   InterPro; IPR015655; PP2C.
DR   InterPro; IPR000222; PP2C_BS.
DR   InterPro; IPR036457; PPM-type-like_dom_sf.
DR   InterPro; IPR001932; PPM-type_phosphatase-like_dom.
DR   PANTHER; PTHR47992; PROTEIN PHOSPHATASE; 1.
DR   PANTHER; PTHR47992:SF160; PROTEIN PHOSPHATASE 2C 77; 1.
DR   Pfam; PF00481; PP2C; 1.
DR   SMART; SM00331; PP2C_SIG; 1.
DR   SMART; SM00332; PP2Cc; 1.
DR   SUPFAM; SSF81606; PP2C-like; 1.
DR   PROSITE; PS01032; PPM_1; 1.
DR   PROSITE; PS51746; PPM_2; 1.
DR   Genevisible; Q0JLP9; OS.
PE   1: Evidence at protein level;
KW   3D-structure; Abscisic acid signaling pathway; Cytoplasm; Hydrolase;
KW   Magnesium; Manganese; Metal-binding; Nucleus; Protein phosphatase;
KW   Reference proteome.
FT   CHAIN           1..467
FT                   /note="Probable protein phosphatase 2C 6"
FT                   /id="PRO_0000363252"
FT   DOMAIN          149..457
FT                   /note="PPM-type phosphatase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01082"
FT   REGION          61..81
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         205
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         205
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         206
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         386
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         448
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   STRAND          142..144
FT                   /evidence="ECO:0007829|PDB:4OIC"
FT   STRAND          149..155
FT                   /evidence="ECO:0007829|PDB:4OIC"
FT   STRAND          159..161
FT                   /evidence="ECO:0007829|PDB:4OIC"
FT   STRAND          164..176
FT                   /evidence="ECO:0007829|PDB:4OIC"
FT   HELIX           178..181
FT                   /evidence="ECO:0007829|PDB:4OIC"
FT   TURN            191..193
FT                   /evidence="ECO:0007829|PDB:4OIC"
FT   STRAND          195..210
FT                   /evidence="ECO:0007829|PDB:4OIC"
FT   HELIX           211..234
FT                   /evidence="ECO:0007829|PDB:4OIC"
FT   HELIX           239..241
FT                   /evidence="ECO:0007829|PDB:4OIC"
FT   HELIX           245..264
FT                   /evidence="ECO:0007829|PDB:4OIC"
FT   STRAND          280..288
FT                   /evidence="ECO:0007829|PDB:4OIC"
FT   STRAND          290..300
FT                   /evidence="ECO:0007829|PDB:4OIC"
FT   STRAND          302..307
FT                   /evidence="ECO:0007829|PDB:4OIC"
FT   STRAND          310..313
FT                   /evidence="ECO:0007829|PDB:4OIC"
FT   HELIX           323..331
FT                   /evidence="ECO:0007829|PDB:4OIC"
FT   STRAND          336..344
FT                   /evidence="ECO:0007829|PDB:4OIC"
FT   TURN            345..347
FT                   /evidence="ECO:0007829|PDB:4OIC"
FT   HELIX           357..359
FT                   /evidence="ECO:0007829|PDB:4OIC"
FT   TURN            360..362
FT                   /evidence="ECO:0007829|PDB:4OIC"
FT   STRAND          368..373
FT                   /evidence="ECO:0007829|PDB:4OIC"
FT   STRAND          378..384
FT                   /evidence="ECO:0007829|PDB:4OIC"
FT   HELIX           386..389
FT                   /evidence="ECO:0007829|PDB:4OIC"
FT   HELIX           394..411
FT                   /evidence="ECO:0007829|PDB:4OIC"
FT   HELIX           428..443
FT                   /evidence="ECO:0007829|PDB:4OIC"
FT   STRAND          450..456
FT                   /evidence="ECO:0007829|PDB:4OIC"
SQ   SEQUENCE   467 AA;  48635 MW;  50CB687EAB641C5B CRC64;
     MEDVAVAAAL APAPATAPVF SPAAAGLTLI AAAAADPIAA VVAGAMDGVV TVPPVRTASA
     VEDDAVAPGR GEEGGEASAV GSPCSVTSDC SSVASADFEG VGLGFFGAAA DGGAAMVFED
     SAASAATVEA EARVAAGARS VFAVECVPLW GHKSICGRRP EMEDAVVAVS RFFDIPLWML
     TGNSVVDGLD PMSFRLPAHF FGVYDGHGGA QVANYCRERL HAALVEELSR IEGSVSGANL
     GSVEFKKKWE QAFVDCFSRV DEEVGGNASR GEAVAPETVG STAVVAVICS SHIIVANCGD
     SRAVLCRGKQ PVPLSVDHKP NREDEYARIE AEGGKVIQWN GYRVFGVLAM SRSIGDRYLK
     PWIIPVPEIT IVPRAKDDEC LVLASDGLWD VMSNEEVCDV ARKRILLWHK KNGTNPASAP
     RSGDSSDPAA EAAAECLSKL ALQKGSKDNI SVIVVDLKAH RKFKSKS
//