Q0JL46 (NCASE_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neutral ceramidase
Short name=N-CDase
Short name=NCDase
Short name=OsCDase
EC=3.5.1.23

Alternative name(s):
Acylsphingosine deacylase
N-acylsphingosine amidohydrolase

Gene names

Ordered Locus Names:	Os01g0624000, LOC_Os01g43520
ORF Names:	OsJ_02661, P0501G01.30-1

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	785 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid. Uses ceramide instead of phytoceramide as substrate. Ref.1
Catalytic activity	N-acylsphingosine + H2O = a carboxylate + sphingosine.
Enzyme regulation	Enhanced activity in the presence of calcium, magnesium, manganese and zinc ions, but inhibited activity in the presence of iron ion. Ref.1
Subcellular location	Secreted By similarity. Endoplasmic reticulum. Golgi apparatus Ref.1.
Tissue specificity	Expressed in seedlings, with higher levels in roots than in shoots. Ref.1
Sequence similarities	Belongs to the neutral ceramidase family.
Biophysicochemical properties	Kinetic parameters: KM=39.34 µM for D-erythro-C12-NBD-ceramide (at pH 5.7 and 37 degrees Celsius) Ref.1 Vmax=0.079 pmol/h/µg enzyme with D-erythro-C12-NBD-ceramide as substrate pH dependence: Optimum pH is 5.7-6.
Sequence caution	The sequence BAD61179.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence EEE55011.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Golgi apparatus Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	ceramidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q0JL46-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 35	35	Potential
	Chain	36 – 785	750	Neutral ceramidase		PRO_0000403419
Sites
	Active site	359	1	Nucleophile By similarity
Amino acid modifications
	Glycosylation	31	1	N-linked (GlcNAc...) Potential
	Glycosylation	377	1	N-linked (GlcNAc...) Potential
	Glycosylation	675	1	N-linked (GlcNAc...) Potential
	Glycosylation	685	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: C8AD952C8A3053C0	FASTA	785	86,593
	10 20 30 40 50 60 MEASSWLCYQ ARGFGSSRVW LWLLLALVLL NCSLVLSASP YLVGMGSFDI TGPAADVNMM 70 80 90 100 110 120 GYANTEQIAS GIHFRLKSRA FIVAEPNGKR VVFVNIDACM ASQIVTIKVL ERLKARYGDL 130 140 150 160 170 180 YNENNVAISG IHTHAGPGGY LQYVVYIVTS LGFVRQSFDV IVDGIEQSIV EAHNNLRPGK 190 200 210 220 230 240 IFVNKGDLLD AGVNRSPSAY LNNPAEERSK YEYNVDKEMT LIKFVDDELG PVGSFNWFAT 250 260 270 280 290 300 HGTSMSRTNS LISGDNKGAA ARFMEDWAEQ MGLPKQSAHA NSDDLRSLHK TSVLPRRVST 310 320 330 340 350 360 IIPEPNEITD DLIQLASSYE ASGGRRLAGS SITRRIRSTQ QNKPKFVSAF CQSNCGDVSP 370 380 390 400 410 420 NVLGTFCIDT NLPCDFNHST CNGKNELCYG RGPGYPDEFE STRVIGNRQF LKARDLFDSA 430 440 450 460 470 480 SEEIQGKIDY RHTYLDFSKL EVKVSTSAGG QQTVKTCPAA MGFAFAAGTT DGPGAFDFRQ 490 500 510 520 530 540 GDVKGNPFWK LVRNLLKTPG KDQVECHSPK PILLDTGEMK EPYDWAPAIL PVQMIRIGQL 550 560 570 580 590 600 VILCVPGEFT TMAGRRLRDA VKTVLTSGNS EFDKNIHVVL AGLTNSYSQY ITTFEEYQIQ 610 620 630 640 650 660 RYEGASTLYG PHTLSAYIQE FQKLAMAMIA NKEVPTNFQP PDMLDKQIGL LPGVVFDSTP 670 680 690 700 710 720 LGVKFGDVNS DVPGNSTFNK GSTVNATFYS ACPRNDLLTD GTFALVEKLD GNNNWVPVYD 730 740 750 760 770 780 DDDWSLRFKW SRPARLSSRS FATLEWTVPE DAAAGVYRLR HFGASKPMFG SVRHFTGTSR AFAVR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of OsCDase, a ceramidase enzyme from rice." Pata M.O., Wu B.X., Bielawski J., Xiong T.C., Hannun Y.A., Ng C.K.-Y. Plant J. 55:1000-1009(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: cv. Nipponbare.
[2]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[5]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[6]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EU422991 mRNA. Translation: ACA49516.1. AP002819 Genomic DNA. Translation: BAD61179.1. Sequence problems. AP008207 Genomic DNA. Translation: BAF05532.1. CM000138 Genomic DNA. Translation: EEE55011.1. Sequence problems. AK099625 mRNA. Translation: BAG94227.1.
RefSeq	NP_001043618.1. NM_001050153.1.
UniGene	Os.23622.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	39947.LOC_Os01g43520.2.
Proteomic databases
PRIDE	Q0JL46.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os01g43520.1; LOC_Os01g43520.1; LOC_Os01g43520. LOC_Os01g43520.2; LOC_Os01g43520.2; LOC_Os01g43520.
GeneID	4326680.
KEGG	osa:4326680.
Organism-specific databases
Gramene	Q0JL46.
Phylogenomic databases
eggNOG	NOG75118.
HOGENOM	HOG000209915.
KO	K12349.
OMA	SWFAVHP.
ProtClustDB	CLSN2682562.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-15591.
Gene expression databases
ArrayExpress	Q0JL46.
Family and domain databases
InterPro	IPR006823. Ceramidase_alk. [Graphical view]
PANTHER	PTHR12670. PTHR12670. 1 hit.
Pfam	PF04734. Ceramidase_alk. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NCASE_ORYSJ

Accession

Primary (citable) accession number: Q0JL46
Secondary accession number(s): B9EY48, Q5ZE61

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2011
Last sequence update:	October 3, 2006
Last modified:	April 3, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program