Q0JKM9 (MAN1_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mannan endo-1,4-beta-mannosidase 1
EC=3.2.1.78

Alternative name(s):
Beta-mannanase 1
Endo-beta-1,4-mannanase 1
OsMAN1

Gene names

Name:	MAN1
Ordered Locus Names:	Os01g0663300, LOC_Os01g47400
ORF Names:	P0671D01.40

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.
Subcellular location	Secreted Potential.
Tissue specificity	Ubiquitous. Ref.5
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	mannan endo-1,4-beta-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q0JKM9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q0JKM9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     318-346: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Potential
	Chain	29 – 432	404	Mannan endo-1,4-beta-mannosidase 1		PRO_0000277482
Sites
	Active site	210	1	Proton donor By similarity
	Active site	329	1	Nucleophile By similarity
Natural variations
	Alternative sequence	318 – 346	29	Missing in isoform 2.		VSP_023020

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 6, 2007. Version 2. Checksum: 170FC7BF13EA51F3	FASTA	432	46,875
	10 20 30 40 50 60 MRLLGAHRAA LLVLACVVVV VIHGLGEAEA LGGGGGFVRA QGTRFVLDGN PYYANGFNAY 70 80 90 100 110 120 WLMLLAADPS QRGKVSAALG EAAGHGLTVA RTWAFSDGGG GNALQLSPGN YNENTFKGLD 130 140 150 160 170 180 FVLSEARKYG IKVILSLVDN YDSFGGRKQY VNWARAQGQG IGSDDEFFTN PVVKGFYKNH 190 200 210 220 230 240 VKTVLTRKNT ITGVAYRDDP TILAWELMNE PRCQSDLSGR TVQSWITEMA AHVKSIDRNH 250 260 270 280 290 300 MLEVGLEGFY GASSPSRIAA VNPSGYQLGT DFIANNQVPG IDFATVHSYP DQWLSGKDDQ 310 320 330 340 350 360 AQLGFMGRWL DAHIADAQAV LRKPLLIAEF GKSWKDPGYS SGQRDALYGT VYAKIYESAR 370 380 390 400 410 420 RGGATVGGLF WQLLVPGMDS YRDGYEVVFG ETPSTTGVIT TNSRRLRFLS KAFARARQAQ 430 PARGKGRHNG GK
	Isoform 2 [UniParc]. Checksum: C47F8DF4FFC576AA	FASTA	403	43,674
	10 20 30 40 50 60 MRLLGAHRAA LLVLACVVVV VIHGLGEAEA LGGGGGFVRA QGTRFVLDGN PYYANGFNAY 70 80 90 100 110 120 WLMLLAADPS QRGKVSAALG EAAGHGLTVA RTWAFSDGGG GNALQLSPGN YNENTFKGLD 130 140 150 160 170 180 FVLSEARKYG IKVILSLVDN YDSFGGRKQY VNWARAQGQG IGSDDEFFTN PVVKGFYKNH 190 200 210 220 230 240 VKTVLTRKNT ITGVAYRDDP TILAWELMNE PRCQSDLSGR TVQSWITEMA AHVKSIDRNH 250 260 270 280 290 300 MLEVGLEGFY GASSPSRIAA VNPSGYQLGT DFIANNQVPG IDFATVHSYP DQWLSGKDDQ 310 320 330 340 350 360 AQLGFMGRWL DAHIADALYG TVYAKIYESA RRGGATVGGL FWQLLVPGMD SYRDGYEVVF 370 380 390 400 GETPSTTGVI TTNSRRLRFL SKAFARARQA QPARGKGRHN GGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Nipponbare.
[5]	"The endo-beta-mannanase gene families in Arabidopsis, rice, and poplar." Yuan J.S., Yang X., Lai J., Lin H., Cheng Z.-M., Nonogaki H., Chen F. Funct. Integr. Genomics 7:1-16(2007) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP003284 Genomic DNA. Translation: BAB91747.1. AP008207 Genomic DNA. Translation: BAF05699.1. AK071667 mRNA. No translation available.
RefSeq	NP_001043785.1. NM_001050320.1.
UniGene	Os.10006.
3D structure databases
ProteinModelPortal	Q0JKM9.
SMR	Q0JKM9. Positions 35-410.
ModBase	Search...
Protein family/group databases
CAZy	GH5. Glycoside Hydrolase Family 5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os01g47400.1; LOC_Os01g47400.1; LOC_Os01g47400.
GeneID	4327860.
KEGG	osa:4327860.
Organism-specific databases
Gramene	Q0JKM9.
Phylogenomic databases
eggNOG	COG3934.
HOGENOM	HOG000169952.
OMA	NRIPGID.
ProtClustDB	CLSN2691665.
Gene expression databases
ArrayExpress	Q0JKM9.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR001547. Glyco_hydro_5. IPR018087. Glyco_hydro_5_CS. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00150. Cellulase. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00659. GLYCOSYL_HYDROL_F5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAN1_ORYSJ

Accession

Primary (citable) accession number: Q0JKM9
Secondary accession number(s): Q8LR27

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	February 6, 2007
Last modified:	May 1, 2013
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program