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Q0JJD4

- MAN2_ORYSJ

UniProt

Q0JJD4 - MAN2_ORYSJ

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Protein

Mannan endo-1,4-beta-mannosidase 2

Gene

MAN2

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei227 – 2271Proton donorBy similarity
Active sitei349 – 3491NucleophileBy similarity

GO - Molecular functioni

  1. mannan endo-1,4-beta-mannosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannan endo-1,4-beta-mannosidase 2 (EC:3.2.1.78)
Alternative name(s):
Beta-mannanase 2
Endo-beta-1,4-mannanase 2
OsMAN2
Gene namesi
Name:MAN2
Ordered Locus Names:Os01g0746700, LOC_Os01g54300
ORF Names:OSJNBa0014K08.42, P0481E12.1
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 1

Organism-specific databases

GrameneiQ0JJD4.

Subcellular locationi

Secreted Curated

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence AnalysisAdd
BLAST
Chaini28 – 445418Mannan endo-1,4-beta-mannosidase 2PRO_0000277483Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi181 – 1811N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Expressed in stems and seeds, and at lower levels in roots and leaves.1 Publication

Gene expression databases

ExpressionAtlasiQ0JJD4. baseline.

Structurei

3D structure databases

ProteinModelPortaliQ0JJD4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3934.
InParanoidiQ0JJD4.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0JJD4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAVGNGLILY HILGLASCIA LVYFSLGEVD LRDALPSLPF SGGASRAAAA
60 70 80 90 100
SLPFVERRGK RLFLDGRPFY INGWNSYWLM DLAVEPNTRP RVSSMFRTAV
110 120 130 140 150
SMGLTVCRTW AFNDGSYNAL QLSPGHFDER VFKALDRVVA EASEHGVRLI
160 170 180 190 200
LSLANNLDAY GGKRQYVRWA WEEGVGLTAS NDSFFFDPAI RDYFKVYLKT
210 220 230 240 250
LLMRKNHLTG LEYRDDPTIL AWELMNEPRC TSDPSGDTLQ RWMEEMSAYV
260 270 280 290 300
KSIDKKHLLT VGTEGFYGPT SSQEKLNINP GEWFPNNYGA DFIRNSKIQD
310 320 330 340 350
IDFASVHVYP DNWLQHASLD EKLKFMTRWI TAHVEDGDGE LEKPVLVTEF
360 370 380 390 400
GLSHQVEGFE DAHRDVLYRA VYDIVHGSAR RGGAAGGALV WQLAAEGMEE
410 420 430 440
YHDGFSIVPS ERPSMMRLIK EQSCRLAAVR YGEEGARKVL KTVCA
Length:445
Mass (Da):50,063
Last modified:February 6, 2007 - v2
Checksum:iC7A62692B7597FC9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP003076 Genomic DNA. Translation: BAB56016.1.
AP003376 Genomic DNA. Translation: BAC05600.1.
AP008207 Genomic DNA. Translation: BAF06144.2.
RefSeqiNP_001044230.2. NM_001050765.2.
UniGeneiOs.2123.

Genome annotation databases

EnsemblPlantsiOS01T0746700-00; OS01T0746700-00; OS01G0746700.
GeneIDi4326178.
KEGGiosa:4326178.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP003076 Genomic DNA. Translation: BAB56016.1 .
AP003376 Genomic DNA. Translation: BAC05600.1 .
AP008207 Genomic DNA. Translation: BAF06144.2 .
RefSeqi NP_001044230.2. NM_001050765.2.
UniGenei Os.2123.

3D structure databases

ProteinModelPortali Q0JJD4.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS01T0746700-00 ; OS01T0746700-00 ; OS01G0746700 .
GeneIDi 4326178.
KEGGi osa:4326178.

Organism-specific databases

Gramenei Q0JJD4.

Phylogenomic databases

eggNOGi COG3934.
InParanoidi Q0JJD4.

Gene expression databases

ExpressionAtlasi Q0JJD4. baseline.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF00150. Cellulase. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence and structure of rice chromosome 1."
    Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.
    , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.
    Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  4. "The endo-beta-mannanase gene families in Arabidopsis, rice, and poplar."
    Yuan J.S., Yang X., Lai J., Lin H., Cheng Z.-M., Nonogaki H., Chen F.
    Funct. Integr. Genomics 7:1-16(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, TISSUE SPECIFICITY.

Entry informationi

Entry nameiMAN2_ORYSJ
AccessioniPrimary (citable) accession number: Q0JJD4
Secondary accession number(s): Q94J47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: February 6, 2007
Last modified: October 29, 2014
This is version 59 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3