Q0JJD4 (MAN2_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mannan endo-1,4-beta-mannosidase 2
EC=3.2.1.78

Alternative name(s):
Beta-mannanase 2
Endo-beta-1,4-mannanase 2
OsMAN2

Gene names

Name:	MAN2
Ordered Locus Names:	Os01g0746700, LOC_Os01g54300
ORF Names:	OSJNBa0014K08.42, P0481E12.1

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.
Subcellular location	Secreted Potential.
Tissue specificity	Expressed in stems and seeds, and at lower levels in roots and leaves. Ref.4
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	mannan endo-1,4-beta-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 445	418	Mannan endo-1,4-beta-mannosidase 2		PRO_0000277483
Sites
	Active site	227	1	Proton donor By similarity
	Active site	349	1	Nucleophile By similarity
Amino acid modifications
	Glycosylation	181	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q0JJD4 [UniParc]. Last modified February 6, 2007. Version 2. Checksum: C7A62692B7597FC9	FASTA	445	50,063
	10 20 30 40 50 60 MAVGNGLILY HILGLASCIA LVYFSLGEVD LRDALPSLPF SGGASRAAAA SLPFVERRGK 70 80 90 100 110 120 RLFLDGRPFY INGWNSYWLM DLAVEPNTRP RVSSMFRTAV SMGLTVCRTW AFNDGSYNAL 130 140 150 160 170 180 QLSPGHFDER VFKALDRVVA EASEHGVRLI LSLANNLDAY GGKRQYVRWA WEEGVGLTAS 190 200 210 220 230 240 NDSFFFDPAI RDYFKVYLKT LLMRKNHLTG LEYRDDPTIL AWELMNEPRC TSDPSGDTLQ 250 260 270 280 290 300 RWMEEMSAYV KSIDKKHLLT VGTEGFYGPT SSQEKLNINP GEWFPNNYGA DFIRNSKIQD 310 320 330 340 350 360 IDFASVHVYP DNWLQHASLD EKLKFMTRWI TAHVEDGDGE LEKPVLVTEF GLSHQVEGFE 370 380 390 400 410 420 DAHRDVLYRA VYDIVHGSAR RGGAAGGALV WQLAAEGMEE YHDGFSIVPS ERPSMMRLIK 430 440 EQSCRLAAVR YGEEGARKVL KTVCA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"The endo-beta-mannanase gene families in Arabidopsis, rice, and poplar." Yuan J.S., Yang X., Lai J., Lin H., Cheng Z.-M., Nonogaki H., Chen F. Funct. Integr. Genomics 7:1-16(2007) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP003076 Genomic DNA. Translation: BAB56016.1. AP003376 Genomic DNA. Translation: BAC05600.1. AP008207 Genomic DNA. Translation: BAF06144.2.
RefSeq	NP_001044230.2. NM_001050765.2.
UniGene	Os.2123.
3D structure databases
ProteinModelPortal	Q0JJD4.
ModBase	Search...
Protein family/group databases
CAZy	GH5. Glycoside Hydrolase Family 5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4326178.
KEGG	dosa:Os01t0746700-00. osa:4326178.
Organism-specific databases
Gramene	Q0JJD4.
Phylogenomic databases
eggNOG	COG3934.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR001547. Glyco_hydro_5. IPR018087. Glyco_hydro_5_CS. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00150. Cellulase. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00659. GLYCOSYL_HYDROL_F5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAN2_ORYSJ

Accession

Primary (citable) accession number: Q0JJD4
Secondary accession number(s): Q94J47

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	February 6, 2007
Last modified:	May 1, 2013
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program