Reviewed, UniProtKB/Swiss-Prot Q0JHP8 (LAC8_ORYSJ)

Names and origin

Protein names

Recommended name:
    Laccase-8
    EC=1.10.3.2
Alternative name(s):
    Benzenediol:oxygen oxidoreductase 8
    Urishiol oxidase 8
    Diphenol oxidase 8

Gene names

Name:	LAC8
Ordered Locus Names:	Os01g0850800, LOC_Os01g63200
ORF Names:	P0529H11.24

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	554 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Lignin degradation and detoxification of lignin-derived products By similarity.
Catalytic activity	4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O.
Cofactor	Binds 4 copper ions per monomer By similarity.
Subcellular location	Secreted › extracellular space › apoplastPotential.
Sequence similarities	Belongs to the multicopper oxidase family. Contains 3 plastocyanin-like domains.
Sequence caution	The sequence BAD82649.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAF06730.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Lignin degradation
Cellular component	Apoplast Secreted
Domain	Repeat Signal
Ligand	Copper Metal-binding
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	lignin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	copper ion binding Inferred from electronic annotation. Source: InterPro laccase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 554	533	Laccase-8		PRO_0000291893
Regions
	Domain	29 – 145	117	Plastocyanin-like 1
	Domain	156 – 309	154	Plastocyanin-like 2
	Domain	411 – 537	127	Plastocyanin-like 3
Sites
	Metal binding	79	1	Copper 1 By similarity
	Metal binding	81	1	Copper 2 By similarity
	Metal binding	124	1	Copper 2 By similarity
	Metal binding	126	1	Copper 3 By similarity
	Metal binding	455	1	Copper 4 By similarity
	Metal binding	458	1	Copper 1 By similarity
	Metal binding	460	1	Copper 3 By similarity
	Metal binding	516	1	Copper 3 By similarity
	Metal binding	517	1	Copper 4 By similarity
	Metal binding	518	1	Copper 2 By similarity
	Metal binding	522	1	Copper 4 By similarity
Amino acid modifications
	Glycosylation	107	1	N-linked (GlcNAc...) Potential
	Glycosylation	113	1	N-linked (GlcNAc...) Potential
	Glycosylation	271	1	N-linked (GlcNAc...) Potential
	Glycosylation	369	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q0JHP8-1 [UniParc]. Last modified June 26, 2007. Version 2. Checksum: 8BBF740424ECC3F4	FASTA	554	60,278
	10 20 30 40 50 60 MASAAMLVPL VLVLCTAAAS AAVVEHTFKV GGTKITQLCM NSVIYTANQQ LPGPTIEVTE 70 80 90 100 110 120 GDTLVVHAVN DSPYPLSLHW HGVYQLRSGW NDGANKITQC PIQPSGNFTY RFNITGQEGT 130 140 150 160 170 180 LWWHAHSSLL RATIYGALII KPRNGPSGYP FPEPYEEIPI LLGEWWNRNV DDVENDGYLT 190 200 210 220 230 240 GLGPQISDAL TINGMPGDQN RCKGSAMYEV EVEYGKTCLL RIINAAVNVE LFFKVAGHTF 250 260 270 280 290 300 TVVAADASYT KPYATDVIVI APGQTVDALM NTTASPGRYY MAAHVFDSKT VAVPFDQSTA 310 320 330 340 350 360 TGIVKYKGVP NYAPAAMPSL PPHDDVVTAG RFYWSLTGLA RPSDPGVPTT VDHNMVVTFG 370 380 390 400 410 420 LDQAPCAPNQ TKCSGFALVA AMNRNSFQFP DQKVSLLEAL YKGVPGVYSE DFPDFPPPMQ 430 440 450 460 470 480 GFRKATAVKK VKYNDVVEVV LQSEQYSSTL GTENHPIHLH GFDFYLLAQG LGRFNPSMKS 490 500 510 520 530 540 KYNLVDPQVR NTVAVPAGGW AVIRFMANNP GMWFMHCHLD AHLPLGLAMV FEVLNGPAPN 550 LLPPPPVDHP KCHG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP004072 Genomic DNA. Translation: BAD82649.1. Sequence problems. AP008207 Genomic DNA. Translation: BAF06730.1. Sequence problems.
RefSeq	NP_001044816.1.
UniGene	Os.91169
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4324803.
KEGG	osa:4324803.
NMPDR	fig|39947.1.peg.7578.
Organism-specific databases
Gramene	Q5N7A2.
Family and domain databases
InterPro	IPR001117. Cu-oxidase. IPR011707. Cu-oxidase_3. IPR002355. Cu_oxidase_Cu_BS. IPR008972. Cupredoxin. IPR017761. Laccase. [Graphical view]
Gene3D	G3DSA:2.60.40.420. Cupredoxin. 1 hit.
Pfam	PF00394. Cu-oxidase. 1 hit. PF07732. Cu-oxidase_3. 1 hit. [Graphical view]
PROSITE	PS00079. MULTICOPPER_OXIDASE1. False negative. PS00080. MULTICOPPER_OXIDASE2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LAC8_ORYSJ

Accession

Primary (citable) accession number: Q0JHP8
Secondary accession number(s): Q5N7A2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	June 26, 2007
Last modified:	November 25, 2008
This is version 21 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)