ID   F16P2_ORYSJ             Reviewed;         339 AA.
AC   Q0JHF8; O64421; Q5N9E2; Q94JN1;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 2.
DT   16-JUN-2009, entry version 24.
DE   RecName: Full=Fructose-1,6-bisphosphatase, cytosolic;
DE            Short=FBPase;
DE            EC=3.1.3.11;
DE   AltName: Full=D-fructose-1,6-bisphosphate 1-phosphohydrolase;
GN   OrderedLocusNames=Os01g0866400, LOC_Os01g64660; ORFNames=P0505D12.36;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade;
OC   Ehrhartoideae; Oryzeae; Oryza.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Nipponbare; TISSUE=Leaf;
RA   Takahashi S., Hirose T., Imaizumi N., Ohsugi R.;
RT   "Cloning of a cDNA encoding cytosolic fructose-1,6-bisphosphatase from
RT   rice (Oryza sativa L.).";
RL   (er) Plant Gene Register PGR98-084.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   MEDLINE=22337376; PubMed=12447438; DOI=10.1038/nature01184;
RA   Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA   Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA   Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M.,
RA   Okamoto M., Ando T., Aoki H., Arita K., Hamada M., Harada C.,
RA   Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M.,
RA   Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K.,
RA   Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I.,
RA   Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y.,
RA   Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M.,
RA   Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M.,
RA   Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K.,
RA   Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K.,
RA   Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y.,
RA   Yano M., Jiang J., Gojobori T.;
RT   "The genome sequence and structure of rice chromosome 1.";
RL   Nature 420:312-316(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=17210932; DOI=10.1101/gr.5509507;
RG   The rice annotation project (RAP);
RT   "Curated genome annotation of Oryza sativa ssp. japonica and
RT   comparative genome analysis with Arabidopsis thaliana.";
RL   Genome Res. 17:175-183(2007).
CC   -!- CATALYTIC ACTIVITY: D-fructose 1,6-bisphosphate + H(2)O = D-
CC       fructose 6-phosphate + phosphate.
CC   -!- COFACTOR: Binds 3 magnesium ions per subunit (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- MISCELLANEOUS: In plants there are two FBPase isozymes: one in the
CC       cytosol and the other in the chloroplast.
CC   -!- SIMILARITY: Belongs to the FBPase class 1 family.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAF06820.1; Type=Erroneous gene model prediction;
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DR   EMBL; AB007193; BAA25422.1; -; mRNA.
DR   EMBL; AP003270; BAD81916.1; -; Genomic_DNA.
DR   EMBL; AP008207; BAF06820.1; ALT_SEQ; Genomic_DNA.
DR   RefSeq; NP_001044906.1; -.
DR   UniGene; Os.3408; -.
DR   HSSP; P00636; 1FJ9.
DR   GeneID; 4325071; -.
DR   KEGG; osa:4325071; -.
DR   Gramene; O64421; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0042132; F:fructose 1,6-bisphosphate 1-phosphatase act...; IEA:EC.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR000146; Fructose_bisphosphatase.
DR   InterPro; IPR017955; IMPase/FBPase.
DR   PANTHER; PTHR11556; In_FB_phphtase; 1.
DR   Pfam; PF00316; FBPase; 1.
DR   PRINTS; PR00115; FBPHPHTASE.
DR   PRINTS; PR00377; INFBPHPHTASE.
DR   ProDom; PD001491; In_FB_phphtase; 1.
DR   PROSITE; PS00124; FBPASE; 1.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Cytoplasm; Hydrolase; Magnesium;
KW   Metal-binding.
FT   CHAIN         1    339       Fructose-1,6-bisphosphatase, cytosolic.
FT                                /FTId=PRO_0000200517.
FT   REGION      124    127       Substrate binding (By similarity).
FT   METAL        71     71       Magnesium 1 (By similarity).
FT   METAL       100    100       Magnesium 1 (By similarity).
FT   METAL       100    100       Magnesium 2 (By similarity).
FT   METAL       121    121       Magnesium 2 (By similarity).
FT   METAL       121    121       Magnesium 3 (By similarity).
FT   METAL       123    123       Magnesium 2; via carbonyl oxygen (By
FT                                similarity).
FT   METAL       124    124       Magnesium 3 (By similarity).
FT   METAL       283    283       Magnesium 3 (By similarity).
FT   BINDING     215    215       Substrate (By similarity).
FT   BINDING     247    247       Substrate (By similarity).
FT   BINDING     267    267       Substrate (By similarity).
FT   BINDING     277    277       Substrate (By similarity).
SQ   SEQUENCE   339 AA;  37035 MW;  C87583E7132E8AF3 CRC64;
     MDHEADAYRT DLMTITRYVL NEQSRNPEAR GDLTILLSHI VLGCKFVASA VNKAGLAKLI
     GLAGETNVQG EEQKKLDVLS NEVFVKALVS SGRTCVLVSE EDEEATFVDP ALRGKYCVCF
     DPLDGSSNID CGVSIGTIFG IYMIKDKENV TLEDVLQPGK NMVAAGYCMY GSSCTLVLST
     GNGVNGFTLD PSLGEFILTH PDIKIPKKGK IYSVNEGNAK NWDEPTAKFV EKCKFPKDGS
     SPKSLRYIGS MVADVHRTLL YGGVFLYPAD KKSPNGKLRV LYEVFPMSFL MEQAGGQSFT
     GKERALDLVP TKIHERSPIF LGSFEDVEEI KGLYAAQAK
//