Reviewed, UniProtKB/Swiss-Prot Q0JHF8 (F16P2_ORYSJ)

Names and origin

Protein names

Recommended name:
    Fructose-1,6-bisphosphatase, cytosolic
      Short name=FBPase
    EC=3.1.3.11
Alternative name(s):
    D-fructose-1,6-bisphosphate 1-phosphohydrolase

Gene names

Ordered Locus Names:	Os01g0866400, LOC_Os01g64660
ORF Names:	P0505D12.36

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.
Cofactor	Binds 3 magnesium ions per subunit By similarity.
Subcellular location	Cytoplasm.
Miscellaneous	In plants there are two FBPase isozymes: one in the cytosol and the other in the chloroplast.
Sequence similarities	Belongs to the FBPase class 1 family.
Sequence caution	The sequence BAF06820.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	fructose 1,6-bisphosphate 1-phosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Fructose-1,6-bisphosphatase, cytosolic		PRO_0000200517
Regions
	Region	124 – 127	4	Substrate binding By similarity
Sites
	Metal binding	71	1	Magnesium 1 By similarity
	Metal binding	100	1	Magnesium 1 By similarity
	Metal binding	100	1	Magnesium 2 By similarity
	Metal binding	121	1	Magnesium 2 By similarity
	Metal binding	121	1	Magnesium 3 By similarity
	Metal binding	123	1	Magnesium 2; via carbonyl oxygen By similarity
	Metal binding	124	1	Magnesium 3 By similarity
	Metal binding	283	1	Magnesium 3 By similarity
	Binding site	215	1	Substrate By similarity
	Binding site	247	1	Substrate By similarity
	Binding site	267	1	Substrate By similarity
	Binding site	277	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0JHF8-1 [UniParc]. Last modified July 10, 2007. Version 2. Checksum: C87583E7132E8AF3	FASTA	339	37,035
	10 20 30 40 50 60 MDHEADAYRT DLMTITRYVL NEQSRNPEAR GDLTILLSHI VLGCKFVASA VNKAGLAKLI 70 80 90 100 110 120 GLAGETNVQG EEQKKLDVLS NEVFVKALVS SGRTCVLVSE EDEEATFVDP ALRGKYCVCF 130 140 150 160 170 180 DPLDGSSNID CGVSIGTIFG IYMIKDKENV TLEDVLQPGK NMVAAGYCMY GSSCTLVLST 190 200 210 220 230 240 GNGVNGFTLD PSLGEFILTH PDIKIPKKGK IYSVNEGNAK NWDEPTAKFV EKCKFPKDGS 250 260 270 280 290 300 SPKSLRYIGS MVADVHRTLL YGGVFLYPAD KKSPNGKLRV LYEVFPMSFL MEQAGGQSFT 310 320 330 GKERALDLVP TKIHERSPIF LGSFEDVEEI KGLYAAQAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a cDNA encoding cytosolic fructose-1,6-bisphosphatase from rice (Oryza sativa L.)." Takahashi S., Hirose T., Imaizumi N., Ohsugi R. Plant Gene Register PGR98-084 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nipponbare. Tissue: Leaf.
[2]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB007193 mRNA. Translation: BAA25422.1. AP003270 Genomic DNA. Translation: BAD81916.1. AP008207 Genomic DNA. Translation: BAF06820.1. Sequence problems.
RefSeq	NP_001044906.1.
UniGene	Os.3408
3D structure databases
SMR	Q0JHF8. Positions 11-337.
ModBase	Search...
Genome annotation databases
GeneID	4325071.
KEGG	osa:4325071.
Organism-specific databases
Gramene	O64421.
Phylogenomic databases
HOGENOM	HBG731261.
Family and domain databases
InterPro	IPR000146. Fructose_bisphosphatase. IPR020548. Fructose_bisphosphatase_AS. [Graphical view]
PANTHER	PTHR11556. In_FB_phphtase. 1 hit.
Pfam	PF00316. FBPase. 1 hit. [Graphical view]
PRINTS	PR00115. F16BPHPHTASE.
PROSITE	PS00124. FBPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

F16P2_ORYSJ

Accession

Primary (citable) accession number: Q0JHF8
Secondary accession number(s): O64421, Q5N9E2, Q94JN1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	July 10, 2007
Last modified:	January 19, 2010
This is version 30 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)