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Protein

Gibberellin 20 oxidase 2

Gene

20ox2

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Key oxidase enzyme in the biosynthesis of gibberellin that catalyzes the conversion of GA53 to GA20 via a three-step oxidation at C-20 of the GA skeleton.1 Publication

Miscellaneous

A second gene, 20ox1, is preferentially expressed in the reproductive organs and enables the flowers in sd1 plants to develop and be fertilized normally.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi249IronPROSITE-ProRule annotation1
Metal bindingi251IronPROSITE-ProRule annotation1
Metal bindingi305IronPROSITE-ProRule annotation1
Active sitei315Sequence analysis1

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
LigandIron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Gibberellin 20 oxidase 2 (EC:1.14.11.-)
Alternative name(s):
GA 20-oxidase 2
Gibberellin C-20 oxidase 2
Os20ox2
Protein semidwarf-1
Gene namesi
Name:20ox2
Synonyms:C20ox2, GA20, Sd-1
Ordered Locus Names:Os01g0883800, LOC_Os01g66100
ORF Names:B1065E10.46
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 1

Pathology & Biotechi

Biotechnological usei

This gene, also known as the 'green revolution gene' has been introduced by conventional breeding procedures into several cultivars. It provides rice cultivars with short, thick culms, raises the harvest index, improves lodging resistance and responsiveness to nitrogen fertilizer, resulting high yields without affecting panicle and grain quality.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002195211 – 389Gibberellin 20 oxidase 2Add BLAST389

Proteomic databases

PaxDbiQ0JH50.

Expressioni

Tissue specificityi

Strongly expressed in leaf blades, stems and unopened flowers. Lower expression in leaf sheath, rachis, shoot apex and roots.1 Publication

Developmental stagei

Expressed within 48 hours after sowing, as well as in 10-day-old plants and flowering panicles.1 Publication

Gene expression databases

ExpressionAtlasiQ0JH50. baseline and differential.

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os01g66100.1.

Structurei

3D structure databases

ProteinModelPortaliQ0JH50.
SMRiQ0JH50.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini224 – 324Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST101

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0143. Eukaryota.
COG3491. LUCA.
InParanoidiQ0JH50.
OMAiWPHEEAR.
OrthoDBiEOG09360BJH.

Family and domain databases

Gene3Di2.60.120.330. 1 hit.
InterProiView protein in InterPro
IPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
PfamiView protein in Pfam
PF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
PROSITEiView protein in PROSITE
PS51471. FE2OG_OXY. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0JH50-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAEHPTPPQ PHQPPPMDST AGSGIAAPAA AAVCDLRMEP KIPEPFVWPN
60 70 80 90 100
GDARPASAAE LDMPVVDVGV LRDGDAEGLR RAAAQVAAAC ATHGFFQVSE
110 120 130 140 150
HGVDAALARA ALDGASDFFR LPLAEKRRAR RVPGTVSGYT SAHADRFASK
160 170 180 190 200
LPWKETLSFG FHDRAAAPVV ADYFSSTLGP DFAPMGRVYQ KYCEEMKELS
210 220 230 240 250
LTIMELLELS LGVERGYYRE FFADSSSIMR CNYYPPCPEP ERTLGTGPHC
260 270 280 290 300
DPTALTILLQ DDVGGLEVLV DGEWRPVSPV PGAMVINIGD TFMALSNGRY
310 320 330 340 350
KSCLHRAVVN QRRERRSLAF FLCPREDRVV RPPPSAATPQ HYPDFTWADL
360 370 380
MRFTQRHYRA DTRTLDAFTR WLAPPAADAA ATAQVEAAS
Length:389
Mass (Da):42,513
Last modified:October 3, 2006 - v1
Checksum:i01B26E496DF2EAC1
GO

Polymorphismi

Sd-1 is the major semidwarfing allele extensively used in modern rice cultivars.1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti94G → V in strain: cv. Jikkoku; semidwarfing. 1 Publication1
Natural varianti266L → F in strain: cv. Calrose76; semidwarfing. 2 Publications1
Natural varianti349D → H in strain: cv. Reimei; semidwarfing. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB077025 mRNA. Translation: BAB89356.1.
AF465255 Genomic DNA. Translation: AAL87949.1.
AP003561 Genomic DNA. Translation: BAB90378.1.
AP008207 Genomic DNA. Translation: BAF06928.1.
AP014957 Genomic DNA. Translation: BAS75582.1.
RefSeqiXP_015627721.1. XM_015772235.1.
UniGeneiOs.19823.

Genome annotation databases

EnsemblPlantsiOS01T0883800-02; OS01T0883800-02; OS01G0883800.
GeneIDi4325003.
GrameneiOS01T0883800-02; OS01T0883800-02; OS01G0883800.
KEGGiosa:4325003.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiGAOX2_ORYSJ
AccessioniPrimary (citable) accession number: Q0JH50
Secondary accession number(s): Q6YLX3
, Q7EC22, Q7GBH4, Q8RVF5, Q8S492
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 3, 2006
Last modified: August 30, 2017
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families