ID   SCRK1_ORYSJ             Reviewed;         323 AA.
AC   Q0JGZ6; Q5JLV6; Q7F445; Q944F4;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 2.
DT   25-NOV-2008, entry version 18.
DE   RecName: Full=Fructokinase-1;
DE            EC=2.7.1.4;
DE   AltName: Full=Fructokinase I;
DE   AltName: Full=OsFKI;
GN   Name=FRK1; Synonyms=FKI;
GN   OrderedLocusNames=Os01g0894300, LOC_Os01g66940;
GN   ORFNames=P0696G06.9, B1078G07.45-1, B1078G07.45-2;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade;
OC   Ehrhartoideae; Oryzeae; Oryza.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   MEDLINE=22337376; PubMed=12447438; DOI=10.1038/nature01184;
RA   Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA   Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA   Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M.,
RA   Okamoto M., Ando T., Aoki H., Arita K., Hamada M., Harada C.,
RA   Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M.,
RA   Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K.,
RA   Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I.,
RA   Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y.,
RA   Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M.,
RA   Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M.,
RA   Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K.,
RA   Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K.,
RA   Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y.,
RA   Yano M., Jiang J., Gojobori T.;
RT   "The genome sequence and structure of rice chromosome 1.";
RL   Nature 420:312-316(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=17210932; DOI=10.1101/gr.5509507;
RG   The rice annotation project (RAP);
RT   "Curated genome annotation of Oryza sativa ssp. japonica and
RT   comparative genome analysis with Arabidopsis thaliana.";
RL   Genome Res. 17:175-183(2007).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   STRAIN=cv. Nipponbare;
RX   MEDLINE=22752273; PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [5]
RP   BIOPHYSICOCHEMICAL PROPERTIES.
RX   MEDLINE=22364004; PubMed=12475618; DOI=10.1016/S0031-9422(02)00428-4;
RA   Jiang H., Dian W., Liu F., Wu P.;
RT   "Isolation and characterization of two fructokinase cDNA clones from
RT   rice.";
RL   Phytochemistry 62:47-52(2003).
CC   -!- FUNCTION: May play an important role in maintaining the flux of
CC       carbon towards starch formation. May also be involved in a sugar-
CC       sensing pathway.
CC   -!- CATALYTIC ACTIVITY: ATP + D-fructose = ADP + D-fructose 6-
CC       phosphate.
CC   -!- ENZYME REGULATION: Not inhibited at high fructose concentration.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=3.3 mM for fructose;
CC   -!- PATHWAY: Glycan biosynthesis; starch biosynthesis.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q0JGZ6-1, Q944F4-1;
CC         Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q0JGZ6-2, Q944F4-2;
CC         Sequence=VSP_018208;
CC         Note=No experimental confirmation available;
CC   -!- SIMILARITY: Belongs to the carbohydrate kinase pfkB family.
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DR   EMBL; AP003316; BAC06252.1; -; Genomic_DNA.
DR   EMBL; AP003407; BAB90210.1; -; Genomic_DNA.
DR   EMBL; AP003407; BAD87551.1; -; Genomic_DNA.
DR   EMBL; AP008207; BAF06982.1; -; Genomic_DNA.
DR   EMBL; AK060500; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; AK120887; -; NOT_ANNOTATED_CDS; mRNA.
DR   RefSeq; NP_001045068.1; -.
DR   UniGene; Os.8324; -.
DR   HSSP; P05054; 1RKD.
DR   GeneID; 4324962; -.
DR   KEGG; osa:4324962; -.
DR   NMPDR; fig|39947.1.peg.7205; -.
DR   Gramene; Q944F4; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0008865; F:fructokinase activity; IEA:EC.
DR   GO; GO:0004747; F:ribokinase activity; IEA:InterPro.
DR   GO; GO:0006014; P:D-ribose metabolic process; IEA:InterPro.
DR   InterPro; IPR011611; Carb/pur_kinase.
DR   InterPro; IPR002173; Carboh/pur_kinase_PfkB_CS.
DR   InterPro; IPR002139; Ribokinase.
DR   Pfam; PF00294; PfkB; 1.
DR   PRINTS; PR00990; RIBOKINASE.
DR   PROSITE; PS00583; PFKB_KINASES_1; 1.
DR   PROSITE; PS00584; PFKB_KINASES_2; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; ATP-binding; Carbohydrate metabolism; Kinase;
KW   Nucleotide-binding; Transferase.
FT   CHAIN         1    323       Fructokinase-1.
FT                                /FTId=PRO_0000234064.
FT   VAR_SEQ      18     95       Missing (in isoform 2).
FT                                /FTId=VSP_018208.
SQ   SEQUENCE   323 AA;  34720 MW;  6DE00EDD49DBDDC4 CRC64;
     MAGRSELVVS FGEMLIDFVP TVAGVSLAEA PAFVKAPGGA PANVAIAVAR LGGGAAFVGK
     LGDDEFGRML AAILRDNGVD DGGVVFDAGA RTALAFVTLR ADGEREFMFY RNPSADMLLT
     HAELNVELIK RAAVFHYGSI SLIAEPCRSA HLRAMEIAKE AGALLSYDPN LREALWPSRE
     EARTKILSIW DQADIVKVSE VELEFLTGID SVEDDVVMKL WRPTMKLLLV TLGDQGCKYY
     ARDFRGAVPS YKVQQVDTTG AGDAFVGALL RRIVQDPSSL QDQKKLEEAI KFANACGAIT
     ATKKGAIPSL PTEVEVLKLM ESA
//