Q0JGZ6 (SCRK1_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fructokinase-1
EC=2.7.1.4

Alternative name(s):
Fructokinase I
OsFKI

Gene names

Name:	FRK1
Synonyms:	FKI
Ordered Locus Names:	Os01g0894300, LOC_Os01g66940
ORF Names:	P0696G06.9, B1078G07.45-1, B1078G07.45-2

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play an important role in maintaining the flux of carbon towards starch formation. May also be involved in a sugar-sensing pathway.
Catalytic activity	ATP + D-fructose = ADP + D-fructose 6-phosphate.
Enzyme regulation	Not inhibited at high fructose concentration.
Pathway	Glycan biosynthesis; starch biosynthesis.
Sequence similarities	Belongs to the carbohydrate kinase pfkB family.
Biophysicochemical properties	Kinetic parameters: KM=3.3 mM for fructose Ref.5

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	D-ribose metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW fructokinase activity Inferred from electronic annotation. Source: EC ribokinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q0JGZ6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q0JGZ6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     18-95: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 323	323	Fructokinase-1		PRO_0000234064
Natural variations
	Alternative sequence	18 – 95	78	Missing in isoform 2.		VSP_018208

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 11, 2007. Version 2. Checksum: 6DE00EDD49DBDDC4	FASTA	323	34,720
	10 20 30 40 50 60 MAGRSELVVS FGEMLIDFVP TVAGVSLAEA PAFVKAPGGA PANVAIAVAR LGGGAAFVGK 70 80 90 100 110 120 LGDDEFGRML AAILRDNGVD DGGVVFDAGA RTALAFVTLR ADGEREFMFY RNPSADMLLT 130 140 150 160 170 180 HAELNVELIK RAAVFHYGSI SLIAEPCRSA HLRAMEIAKE AGALLSYDPN LREALWPSRE 190 200 210 220 230 240 EARTKILSIW DQADIVKVSE VELEFLTGID SVEDDVVMKL WRPTMKLLLV TLGDQGCKYY 250 260 270 280 290 300 ARDFRGAVPS YKVQQVDTTG AGDAFVGALL RRIVQDPSSL QDQKKLEEAI KFANACGAIT 310 320 ATKKGAIPSL PTEVEVLKLM ESA
	Isoform 2 [UniParc] [UniParc]. Checksum: D3D73635C2930EEF	FASTA	245	27,199
	10 20 30 40 50 60 MAGRSELVVS FGEMLIDFVT LRADGEREFM FYRNPSADML LTHAELNVEL IKRAAVFHYG 70 80 90 100 110 120 SISLIAEPCR SAHLRAMEIA KEAGALLSYD PNLREALWPS REEARTKILS IWDQADIVKV 130 140 150 160 170 180 SEVELEFLTG IDSVEDDVVM KLWRPTMKLL LVTLGDQGCK YYARDFRGAV PSYKVQQVDT 190 200 210 220 230 240 TGAGDAFVGA LLRRIVQDPS SLQDQKKLEE AIKFANACGA ITATKKGAIP SLPTEVEVLK LMESA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed: 18089549] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: cv. Nipponbare.
[5]	"Isolation and characterization of two fructokinase cDNA clones from rice." Jiang H., Dian W., Liu F., Wu P. Phytochemistry 62:47-52(2003) [PubMed: 12475618] [Abstract] Cited for: BIOPHYSICOCHEMICAL PROPERTIES.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP003316 Genomic DNA. Translation: BAC06252.1. AP003407 Genomic DNA. Translation: BAB90210.1. AP003407 Genomic DNA. Translation: BAD87551.1. AP008207 Genomic DNA. Translation: BAF06982.2. AK060500 mRNA. Translation: BAG87470.1. AK120887 mRNA. No translation available.
RefSeq	NP_001045068.2. NM_001051603.2.
UniGene	Os.8324.
3D structure databases
ProteinModelPortal	Q0JGZ6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0JGZ6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4324962.
KEGG	osa:4324962.
NMPDR	fig|39947.1.peg.7205.
Organism-specific databases
Gramene	Q944F4.
Phylogenomic databases
HOGENOM	HBG697973.
OMA	ALSLWHP.
Family and domain databases
InterPro	IPR011611. Carb/pur_kinase. IPR002173. Carboh/pur_kinase_PfkB_CS. IPR002139. Ribokinase. [Graphical view]
KO	K00847.
Pfam	PF00294. PfkB. 1 hit. [Graphical view]
PRINTS	PR00990. RIBOKINASE.
PROSITE	PS00583. PFKB_KINASES_1. 1 hit. PS00584. PFKB_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SCRK1_ORYSJ

Accession

Primary (citable) accession number: Q0JGZ6
Secondary accession number(s): B7E523 , Q5JLV6, Q7F445, Q944F4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	September 11, 2007
Last modified:	November 16, 2011
This is version 35 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program