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Protein

Probable sucrose-phosphate synthase 1

Gene

SPS1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Plays a role in photosynthetic sucrose synthesis by catalyzing the rate-limiting step of sucrose biosynthesis from UDP-glucose and fructose- 6-phosphate. Involved in the regulation of carbon partitioning in the leaves of plants. May regulate the synthesis of sucrose and therefore play a major role as a limiting factor in the export of photoassimilates out of the leaf. Plays a role for sucrose availability that is essential for plant growth and fiber elongation (By similarity).By similarity

Catalytic activityi

UDP-glucose + D-fructose 6-phosphate = UDP + sucrose 6(F)-phosphate.

Enzyme regulationi

Activity is regulated by phosphorylation and moderated by concentration of metabolites and light.By similarity

Pathwayi: sucrose biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sucrose from D-fructose 6-phosphate and UDP-alpha-D-glucose.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Probable sucrose-phosphate synthase 5 (SPS5), Probable sucrose-phosphate synthase 4 (SPS4), Probable sucrose-phosphate synthase 3 (SPS3), Probable sucrose-phosphate synthase 1 (SPS1), Probable sucrose-phosphate synthase 2 (SPS2)
  2. Probable sucrose-phosphatase 3 (SPP3), Probable sucrose-phosphatase 1 (SPP1), Sucrose-phosphatase 2 (SPP2)
This subpathway is part of the pathway sucrose biosynthesis, which is itself part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sucrose from D-fructose 6-phosphate and UDP-alpha-D-glucose, the pathway sucrose biosynthesis and in Glycan biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.14. 4460.
UniPathwayiUPA00371; UER00545.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable sucrose-phosphate synthase 1 (EC:2.4.1.14)
Alternative name(s):
Sucrose phosphate synthase 1F
Short name:
OsSPS1F
UDP-glucose-fructose-phosphate glucosyltransferase
Gene namesi
Name:SPS1
Ordered Locus Names:Os01g0919400, LOC_Os01g69030
ORF Names:P0678F11.16
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10841084Probable sucrose-phosphate synthase 1PRO_0000204672Add
BLAST

Proteomic databases

PaxDbiQ0JGK4.

Expressioni

Tissue specificityi

Expressed in germinating seeds.1 Publication

Developmental stagei

Highly expressed in source leaves and at low levels in sink leaves.1 Publication

Inductioni

Circadian-regulated, with the highest expression 1 hour before the beginning of light period (in 14 hours light/10 hours dark cycle).1 Publication

Interactioni

Subunit structurei

Homodimer or homotetramer.By similarity

Protein-protein interaction databases

STRINGi39947.LOC_Os01g69030.2.

Structurei

3D structure databases

ProteinModelPortaliQ0JGK4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi22 – 4524Poly-GlyAdd
BLAST
Compositional biasi793 – 7975Poly-Arg

Sequence similaritiesi

Belongs to the glycosyltransferase 1 family.Curated

Phylogenomic databases

eggNOGiKOG0853. Eukaryota.
COG0438. LUCA.
InParanoidiQ0JGK4.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR006380. SPP_N.
IPR000368. Sucrose_synth.
IPR012819. SucrsPsyn_pln.
[Graphical view]
PfamiPF00534. Glycos_transf_1. 1 hit.
PF05116. S6PP. 1 hit.
PF00862. Sucrose_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02468. sucrsPsyn_pln. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0JGK4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGNEWINGY LEAILDSGGA AGGGGGGGGG GGGGGGGGGG GGGGGVDPRS
60 70 80 90 100
PAAGAASPRG PHMNFNPTHY FVEEVVKGVD ESDLHRTWIK VVATRNARER
110 120 130 140 150
STRLENMCWR IWHLARKKKQ LELEGILRIS ARRKEQEQVR RETSEDLAED
160 170 180 190 200
LFEGEKADTV GELAQQDTPM KKKFQRNFSE LTVSWSDENK EKKLYIVLIS
210 220 230 240 250
LHGLVRGDNM ELGRDSDTGG QVKYVVELAR ALAMMPGVYR VDLFTRQVSS
260 270 280 290 300
PEVDWSYGEP TEMLTSGSTD GEGSGESAGA YIVRIPCGPR DKYLRKEALW
310 320 330 340 350
PYLQEFVDGA LAHILNMSKA LGEQVSNGKL VLPYVIHGHY ADAGDVAALL
360 370 380 390 400
SGALNVPMVL TGHSLGRNKL EQIMKQGRMS KEEIDSTYKI MRRIEGEELA
410 420 430 440 450
LDAAELVITS TRQEIDEQWG LYDGFDVKLE KVLRARARRG VSCHGRFMPR
460 470 480 490 500
MVVIPPGMDF SSVVVPEDTS DGDDGKDFEI ASPRSLPPIW AEVMRFLTNP
510 520 530 540 550
HKPMILALSR PDPKKNITTL VKAFGECRPL RELANLILIM GNRDDIDEMS
560 570 580 590 600
AGNASVLTTV LKLIDKYDLY GSVAFPKHHK QSDVPEIYRL TGKMKGVFIN
610 620 630 640 650
PALVEPFGLT LIEAAAHGLP IVATKNGGPV DIKNALNNGL LVDPHDQHAI
660 670 680 690 700
ADALLKLVAD KNLWQECRKN GLRNIQLYSW PEHCRTYLTR IAGCRIRNPR
710 720 730 740 750
WLMDTPADAA AEEEEALEDS LMDVQDLSLR LSIDGERGSS MNDAPSSDPQ
760 770 780 790 800
DSVQRIMNKI KRSSPADTDG AKIPAEAAAT ATSGAMNKYP LLRRRRRLFV
810 820 830 840 850
IAVDCYGDDG SASKRMLQVI QEVFRAVRSD SQMSRISGFA LSTAMPLPET
860 870 880 890 900
LKLLQLGKIP PTDFDALICG SGSEVYYPST AQCVDAGGRL RPDQDYLLHI
910 920 930 940 950
NHRWSHDGAK QTIAKLAHDG SGTNVEPDVE SCNPHCVSFF IKDPNKVRTI
960 970 980 990 1000
DEMRERVRMR GLRCHLMYCR NATRLQVVPL LASRSQALRY LFVRWGLSVG
1010 1020 1030 1040 1050
NMYLIVGEHG DTDHEEMLSG LHKTVIIRGV TEKGSEQLVR SSGSYQREDV
1060 1070 1080
VPSESPLIAF TKGDLKADEI MRALKEVTKA ASGM
Length:1,084
Mass (Da):119,348
Last modified:September 11, 2007 - v2
Checksum:iA42BA599C4DF4556
GO

Sequence cautioni

The sequence BAF07124 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti49 – 491R → S in BAA08304 (Ref. 1) Curated
Sequence conflicti52 – 565AAGAA → TTGTT in BAA08304 (Ref. 1) Curated
Sequence conflicti384 – 3841I → M in BAA08304 (Ref. 1) Curated
Sequence conflicti494 – 4941M → S in BAA08304 (Ref. 1) Curated
Sequence conflicti497 – 4971L → W in BAA08304 (Ref. 1) Curated
Sequence conflicti539 – 5391I → S in BAA08304 (Ref. 1) Curated
Sequence conflicti542 – 5421N → T in BAA08304 (Ref. 1) Curated
Sequence conflicti548 – 5481E → G in BAA08304 (Ref. 1) Curated
Sequence conflicti578 – 5781H → Y in BAA08304 (Ref. 1) Curated
Sequence conflicti623 – 6231A → G in BAA08304 (Ref. 1) Curated
Sequence conflicti767 – 7671D → E in BAA08304 (Ref. 1) Curated
Sequence conflicti950 – 9501I → M in BAA08304 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D45890 Genomic DNA. Translation: BAA08304.1.
AP003437 Genomic DNA. Translation: BAD87626.1.
AP008207 Genomic DNA. Translation: BAF07124.1. Sequence problems.
AP014957 Genomic DNA. No translation available.
AF378184 mRNA. Translation: AAK54855.1.
PIRiT04103.
RefSeqiXP_015621705.1. XM_015766219.1.
XP_015621706.1. XM_015766220.1.
UniGeneiOs.3454.

Genome annotation databases

GeneIDi4324364.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D45890 Genomic DNA. Translation: BAA08304.1.
AP003437 Genomic DNA. Translation: BAD87626.1.
AP008207 Genomic DNA. Translation: BAF07124.1. Sequence problems.
AP014957 Genomic DNA. No translation available.
AF378184 mRNA. Translation: AAK54855.1.
PIRiT04103.
RefSeqiXP_015621705.1. XM_015766219.1.
XP_015621706.1. XM_015766220.1.
UniGeneiOs.3454.

3D structure databases

ProteinModelPortaliQ0JGK4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os01g69030.2.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Proteomic databases

PaxDbiQ0JGK4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi4324364.

Phylogenomic databases

eggNOGiKOG0853. Eukaryota.
COG0438. LUCA.
InParanoidiQ0JGK4.

Enzyme and pathway databases

UniPathwayiUPA00371; UER00545.
BRENDAi2.4.1.14. 4460.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR006380. SPP_N.
IPR000368. Sucrose_synth.
IPR012819. SucrsPsyn_pln.
[Graphical view]
PfamiPF00534. Glycos_transf_1. 1 hit.
PF05116. S6PP. 1 hit.
PF00862. Sucrose_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02468. sucrsPsyn_pln. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSPSA1_ORYSJ
AccessioniPrimary (citable) accession number: Q0JGK4
Secondary accession number(s): Q43010
, Q43802, Q5JLN3, Q94JN0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: September 7, 2016
This is version 63 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.