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Q0JDC5 (INV2_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-fructofuranosidase, insoluble isoenzyme 2

EC=3.2.1.26
Alternative name(s):
Cell wall beta-fructosidase 2
Invertase 2
OsCIN2
Sucrose hydrolase 2
Gene names
Name:CIN2
Ordered Locus Names:Os04g0413500, LOC_Os04g33740
ORF Names:OJ000126_13.8, OsJ_014165
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length598 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May play a role in sucrose partitioning during seed development.

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.

Subcellular location

Secretedextracellular spaceapoplast Probable. Secretedcell wall Probable. Note: Associated to the cell wall Probable.

Tissue specificity

Expressed in leaves and flowers. Weakly expressed in seeds. Ref.1

Developmental stage

Expressed throughout flowering, with higher expression from 1 to 6 days after flowering. Ref.1

Induction

By sucrose in caryopsis from 1 to 2 and from 9 to 10 days after flowering. Ref.1

Sequence similarities

Belongs to the glycosyl hydrolase 32 family.

Sequence caution

The sequence CAD40589.2 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Cellular componentApoplast
Cell wall
Secreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processnectar secretion

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to karrikin

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to wounding

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

starch metabolic process

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Cellular_componentapoplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

cell wall

Inferred from electronic annotation. Source: UniProtKB-SubCell

vacuole

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Molecular_functioninulinase activity

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

levanase activity

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

sucrose alpha-glucosidase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 598573Beta-fructofuranosidase, insoluble isoenzyme 2
PRO_0000033380

Sites

Active site691 By similarity

Amino acid modifications

Glycosylation1641N-linked (GlcNAc...) Potential
Glycosylation1891N-linked (GlcNAc...) Potential
Glycosylation3481N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q0JDC5 [UniParc].

Last modified October 3, 2006. Version 1.
Checksum: DFAE59481FA19A4F

FASTA59866,263
        10         20         30         40         50         60 
MGVLGSRVAW AWLVQLLLLQ QLAGASHVVY DDLELQAAAT TADGVPPSIV DSELRTGYHF 

        70         80         90        100        110        120 
QPPKNWINDP NAPMYYKGWY HLFYQYNPKG AVWGNIVWAH SVSRDLINWV ALKPAIEPSI 

       130        140        150        160        170        180 
RADKYGCWSG SATMMADGTP VIMYTGVNRP DVNYQVQNVA LPRNGSDPLL REWVKPGHNP 

       190        200        210        220        230        240 
VIVPEGGINA TQFRDPTTAW RGADGHWRLL VGSLAGQSRG VAYVYRSRDF RRWTRAAQPL 

       250        260        270        280        290        300 
HSAPTGMWEC PDFYPVTADG RREGVDTSSA VVDAAASARV KYVLKNSLDL RRYDYYTVGT 

       310        320        330        340        350        360 
YDRKAERYVP DDPAGDEHHI RYDYGNFYAS KTFYDPAKRR RILWGWANES DTAADDVAKG 

       370        380        390        400        410        420 
WAGIQAIPRK VWLDPSGKQL LQWPIEEVER LRGKWPVILK DRVVKPGEHV EVTGLQTAQA 

       430        440        450        460        470        480 
DVEVSFEVGS LEAAERLDPA MAYDAQRLCS ARGADARGGV GPFGLWVLAS AGLEEKTAVF 

       490        500        510        520        530        540 
FRVFRPAARG GGAGKPVVLM CTDPTKSSRN PNMYQPTFAG FVDTDITNGK ISLRSLIDRS 

       550        560        570        580        590 
VVESFGAGGK ACILSRVYPS LAIGKNARLY VFNNGKAEIK VSQLTAWEMK KPVMMNGA 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and expression analysis of the cell-wall invertase gene family in rice (Oryza sativa L.)."
Cho J.-I., Lee S.-K., Ko S., Kim H.-K., Jun S.-H., Lee Y.-H., Bhoo S.H., Lee K.-W., An G., Hahn T.-R., Jeon J.-S.
Plant Cell Rep. 24:225-236(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
Strain: cv. Nipponbare.
[2]"Sequence and analysis of rice chromosome 4."
Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y., Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q., Zhang L. expand/collapse author list , Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T., Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R., Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G., Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C., Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J., Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J., Li J., Hong G., Xue Y., Han B.
Nature 420:316-320(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[4]"The rice annotation project database (RAP-DB): 2008 update."
The rice annotation project (RAP)
Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[5]"The genomes of Oryza sativa: a history of duplications."
Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. expand/collapse author list , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY578159 mRNA. Translation: AAT84402.1.
AL662945 Genomic DNA. Translation: CAD40589.2. Sequence problems.
AP008210 Genomic DNA. Translation: BAF14662.1.
CM000141 Genomic DNA. No translation available.
RefSeqNP_001052748.1. NM_001059283.1.
UniGeneOs.50640.

3D structure databases

ProteinModelPortalQ0JDC5.
SMRQ0JDC5. Positions 51-592.
ModBaseSearch...

Protein family/group databases

CAZyGH32. Glycoside Hydrolase Family 32.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsLOC_Os04g33740.1; LOC_Os04g33740.1; LOC_Os04g33740.
GeneID4335790.
KEGGdosa:Os04t0413500-01.
osa:4335790.

Organism-specific databases

GrameneQ0JDC5.

Phylogenomic databases

eggNOGCOG1621.
KOK01193.
OMADVNYQVQ.
ProtClustDBCLSN2692792.

Gene expression databases

ArrayExpressQ0JDC5.

Family and domain databases

Gene3D2.115.10.20. 1 hit.
InterProIPR008985. ConA-like_lec_gl_sf.
IPR001362. Glyco_hydro_32.
IPR018053. Glyco_hydro_32_AS.
IPR013189. Glyco_hydro_32_C.
IPR013148. Glyco_hydro_32_N.
IPR023296. Glyco_hydro_beta-prop.
[Graphical view]
PfamPF08244. Glyco_hydro_32C. 1 hit.
PF00251. Glyco_hydro_32N. 1 hit.
[Graphical view]
SMARTSM00640. Glyco_32. 1 hit.
[Graphical view]
SUPFAMSSF49899. ConA_like_lec_gl. 1 hit.
SSF75005. Glyco_hydro_43_beta-prop. 1 hit.
PROSITEPS00609. GLYCOSYL_HYDROL_F32. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameINV2_ORYSJ
AccessionPrimary (citable) accession number: Q0JDC5
Secondary accession number(s): A3ATP3 expand/collapse secondary AC list , Q56UD4, Q6VGJ3, Q7XVJ4
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: October 3, 2006
Last modified: April 3, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

SIMILARITY comments

Index of protein domains and families