Q0JDC5 (INV2_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-fructofuranosidase, insoluble isoenzyme 2
EC=3.2.1.26

Alternative name(s):
Cell wall beta-fructosidase 2
Invertase 2
OsCIN2
Sucrose hydrolase 2

Gene names

Name:	CIN2
Ordered Locus Names:	Os04g0413500, LOC_Os04g33740
ORF Names:	OJ000126_13.8, OsJ_014165

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	598 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May play a role in sucrose partitioning during seed development.
Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
Subcellular location	Secreted › extracellular space › apoplast Probable. Secreted › cell wall Probable. Note: Associated to the cell wall Probable.
Tissue specificity	Expressed in leaves and flowers. Weakly expressed in seeds. Ref.1
Developmental stage	Expressed throughout flowering, with higher expression from 1 to 6 days after flowering. Ref.1
Induction	By sucrose in caryopsis from 1 to 2 and from 9 to 10 days after flowering. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 32 family.
Sequence caution	The sequence CAD40589.2 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Apoplast Cell wall Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nectar secretion Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to karrikin Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to wounding Inferred from electronic annotation. Source: EnsemblPlants/Gramene starch metabolic process Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Cellular_component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell vacuole Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	inulinase activity Inferred from electronic annotation. Source: EnsemblPlants/Gramene levanase activity Inferred from electronic annotation. Source: EnsemblPlants/Gramene sucrose alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 598	573	Beta-fructofuranosidase, insoluble isoenzyme 2		PRO_0000033380
Sites
	Active site	69	1	By similarity
Amino acid modifications
	Glycosylation	164	1	N-linked (GlcNAc...) Potential
	Glycosylation	189	1	N-linked (GlcNAc...) Potential
	Glycosylation	348	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q0JDC5 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: DFAE59481FA19A4F	FASTA	598	66,263
	10 20 30 40 50 60 MGVLGSRVAW AWLVQLLLLQ QLAGASHVVY DDLELQAAAT TADGVPPSIV DSELRTGYHF 70 80 90 100 110 120 QPPKNWINDP NAPMYYKGWY HLFYQYNPKG AVWGNIVWAH SVSRDLINWV ALKPAIEPSI 130 140 150 160 170 180 RADKYGCWSG SATMMADGTP VIMYTGVNRP DVNYQVQNVA LPRNGSDPLL REWVKPGHNP 190 200 210 220 230 240 VIVPEGGINA TQFRDPTTAW RGADGHWRLL VGSLAGQSRG VAYVYRSRDF RRWTRAAQPL 250 260 270 280 290 300 HSAPTGMWEC PDFYPVTADG RREGVDTSSA VVDAAASARV KYVLKNSLDL RRYDYYTVGT 310 320 330 340 350 360 YDRKAERYVP DDPAGDEHHI RYDYGNFYAS KTFYDPAKRR RILWGWANES DTAADDVAKG 370 380 390 400 410 420 WAGIQAIPRK VWLDPSGKQL LQWPIEEVER LRGKWPVILK DRVVKPGEHV EVTGLQTAQA 430 440 450 460 470 480 DVEVSFEVGS LEAAERLDPA MAYDAQRLCS ARGADARGGV GPFGLWVLAS AGLEEKTAVF 490 500 510 520 530 540 FRVFRPAARG GGAGKPVVLM CTDPTKSSRN PNMYQPTFAG FVDTDITNGK ISLRSLIDRS 550 560 570 580 590 VVESFGAGGK ACILSRVYPS LAIGKNARLY VFNNGKAEIK VSQLTAWEMK KPVMMNGA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and expression analysis of the cell-wall invertase gene family in rice (Oryza sativa L.)." Cho J.-I., Lee S.-K., Ko S., Kim H.-K., Jun S.-H., Lee Y.-H., Bhoo S.H., Lee K.-W., An G., Hahn T.-R., Jeon J.-S. Plant Cell Rep. 24:225-236(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION. Strain: cv. Nipponbare.
[2]	"Sequence and analysis of rice chromosome 4." Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y., Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q., Zhang L. , Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T., Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R., Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G., Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C., Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J., Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J., Li J., Hong G., Xue Y., Han B. Nature 420:316-320(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[5]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY578159 mRNA. Translation: AAT84402.1. AL662945 Genomic DNA. Translation: CAD40589.2. Sequence problems. AP008210 Genomic DNA. Translation: BAF14662.1. CM000141 Genomic DNA. No translation available.
RefSeq	NP_001052748.1. NM_001059283.1.
UniGene	Os.50640.
3D structure databases
ProteinModelPortal	Q0JDC5.
SMR	Q0JDC5. Positions 51-592.
ModBase	Search...
Protein family/group databases
CAZy	GH32. Glycoside Hydrolase Family 32.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os04g33740.1; LOC_Os04g33740.1; LOC_Os04g33740.
GeneID	4335790.
KEGG	dosa:Os04t0413500-01. osa:4335790.
Organism-specific databases
Gramene	Q0JDC5.
Phylogenomic databases
eggNOG	COG1621.
KO	K01193.
OMA	DVNYQVQ.
ProtClustDB	CLSN2692792.
Gene expression databases
ArrayExpress	Q0JDC5.
Family and domain databases
Gene3D	2.115.10.20. 1 hit.
InterPro	IPR008985. ConA-like_lec_gl_sf. IPR001362. Glyco_hydro_32. IPR018053. Glyco_hydro_32_AS. IPR013189. Glyco_hydro_32_C. IPR013148. Glyco_hydro_32_N. IPR023296. Glyco_hydro_beta-prop. [Graphical view]
Pfam	PF08244. Glyco_hydro_32C. 1 hit. PF00251. Glyco_hydro_32N. 1 hit. [Graphical view]
SMART	SM00640. Glyco_32. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit. SSF75005. Glyco_hydro_43_beta-prop. 1 hit.
PROSITE	PS00609. GLYCOSYL_HYDROL_F32. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

INV2_ORYSJ

Accession

Primary (citable) accession number: Q0JDC5
Secondary accession number(s): A3ATP3 , Q56UD4, Q6VGJ3, Q7XVJ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	October 3, 2006
Last modified:	April 3, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program