Q0JC10 (DCAM_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
S-adenosylmethionine decarboxylase proenzyme
Short name=AdoMetDC
Short name=SAMDC
EC=4.1.1.50

Cleaved into the following 2 chains:

1. S-adenosylmethionine decarboxylase alpha chain
2. S-adenosylmethionine decarboxylase beta chain

Gene names

Name:	SAMDC
Ordered Locus Names:	Os04g0498600, LOC_Os04g42090
ORF Names:	OSJNBa0029H02.4, OSJNBa0067K08.23, OsJ_004076

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	S-adenosyl-L-methionine = (5-deoxy-5-adenosyl)(3-aminopropyl)-methylsulfonium salt + CO2.
Cofactor	Pyruvoyl group.
Pathway	Amine and polyamine biosynthesis; S-adenosylmethioninamine biosynthesis; S-adenosylmethioninamine from S-adenosyl-L-methionine: step 1/1.
Post-translational modification	Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The post-translation cleavage follows an unusual pathway, termed non-hydrolytic serinolysis, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl group blocking the N-terminus of the alpha chain By similarity.
Sequence similarities	Belongs to the eukaryotic AdoMetDC family.
Sequence caution	The sequence CAD41510.3 differs from that shown. Reason: Erroneous gene model prediction. The sequence EAZ14251.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Polyamine biosynthesis Spermidine biosynthesis
Ligand	Pyruvate S-adenosyl-L-methionine Schiff base
Molecular function	Decarboxylase Lyase
PTM	Autocatalytic cleavage Zymogen
Gene Ontology (GO)
Biological process	spermidine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW spermine biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	adenosylmethionine decarboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 77	77	S-adenosylmethionine decarboxylase beta chain By similarity		PRO_0000030017
	Chain	78 – 398	321	S-adenosylmethionine decarboxylase alpha chain By similarity		PRO_0000030018
Sites
	Active site	18	1	By similarity
	Active site	21	1	By similarity
	Active site	78	1	Schiff-base intermediate with substrate; via pyruvic acid By similarity
	Active site	92	1	Proton donor; for catalytic activity By similarity
	Active site	243	1	Proton acceptor; for processing activity By similarity
	Active site	256	1	Proton acceptor; for processing activity By similarity
	Site	77 – 78	2	Cleavage (non-hydrolytic); by autolysis By similarity
Amino acid modifications
	Modified residue	78	1	Pyruvic acid (Ser); by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0JC10 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: BC24F359962F8655	FASTA	398	43,283
	10 20 30 40 50 60 MGVLSAADPP PVSAIGFEGY EKRLEITFSE APVFADPDGR GLRALSRAQI DSVLDLARCT 70 80 90 100 110 120 IVSELSNKDF DSYVLSESSL FIYSDKIVIK TCGTTKLLLT IPRILELAEG LSMPLAAVKY 130 140 150 160 170 180 SRGMFIFPSA QPAPHRSFSE EVAVLNRYFG HLKSGGNAYV IGDPAKPGQK WHIYYATQHP 190 200 210 220 230 240 EQPMVTLEMC MTGLDKEKAS VFFKTSADGH TSCAKEMTKL SGISDIIPEM EICDFDFEPC 250 260 270 280 290 300 GYSMNAIHGS AFSTIHVTPE DGFSYASYEV VGFDASTLAY GDLVKRVLRC FGPSEFSVAV 310 320 330 340 350 360 TIFGGHGHAG TWAKELNADA YKCNNMVEQE LPCGGLLIYQ SFDATEDVPV AVGSPKSVLH 370 380 390 CFEAENMVNP APVKEGKLGN LLPWGEDALE ENDGVFDE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of monocot and dicot plant S-adenosyl-L-methionine decarboxylase gene families including identification in the mRNA of a highly conserved pair of upstream overlapping open reading frames." Franceschetti M., Hanfrey C., Scaramagli S., Torrigiani P., Bagni N., Michael A.J. Biochem. J. 353:403-409(2001) [PubMed: 11139406] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nipponbare.
[2]	"Sequence and analysis of rice chromosome 4." Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y., Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q., Zhang L. , Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T., Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R., Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G., Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C., Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J., Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J., Li J., Hong G., Xue Y., Han B. Nature 420:316-320(2002) [PubMed: 12447439] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed: 18089549] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[5]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y07766 mRNA. Translation: CAA69074.2. AL606594 Genomic DNA. Translation: CAD41510.3. Sequence problems. AL606627 Genomic DNA. Translation: CAD41242.2. AP008210 Genomic DNA. Translation: BAF15127.1. CM000138 Genomic DNA. Translation: EAZ14251.1. Sequence problems.
PIR	T04099.
RefSeq	NP_001053213.1. NM_001059748.1.
UniGene	Os.38386. Os.4786. Os.52749.
3D structure databases
ProteinModelPortal	Q0JC10.
SMR	Q0JC10. Positions 79-345.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0JC10.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os04g42090.1; LOC_Os04g42090.1; LOC_Os04g42090. LOC_Os04g42090.2; LOC_Os04g42090.2; LOC_Os04g42090. LOC_Os04g42090.3; LOC_Os04g42090.3; LOC_Os04g42090. LOC_Os04g42090.4; LOC_Os04g42090.4; LOC_Os04g42090. LOC_Os04g42090.5; LOC_Os04g42090.5; LOC_Os04g42090.
GeneID	4336294.
KEGG	osa:4336294.
Organism-specific databases
Gramene	O24215.
Phylogenomic databases
GeneTree	EPGT00050000010946.
PhylomeDB	Q0JC10.
ProtClustDB	PLN02524.
Family and domain databases
InterPro	IPR001985. S-AdoMet_decarboxylase. IPR018167. S-AdoMet_decarboxylase_subgr. IPR016067. S-AdoMet_deCO2ase_core. IPR018166. S-AdoMet_deCO2ase_CS. [Graphical view]
Gene3D	G3DSA:3.60.90.10. SAM_decarbox. 1 hit.
KO	K01611.
PANTHER	PTHR11570. SAM_decarbox. 1 hit.
Pfam	PF01536. SAM_decarbox. 1 hit. [Graphical view]
PIRSF	PIRSF001355. S-AdenosylMet_decarboxylase. 1 hit.
SUPFAM	SSF56276. S-AdenosylMet_decarbase_core. 1 hit.
TIGRFAMs	TIGR00535. SAM_DCase. 1 hit.
PROSITE	PS01336. ADOMETDC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DCAM_ORYSJ

Accession

Primary (citable) accession number: Q0JC10
Secondary accession number(s): A2ZZV4 , O24215, O81269, Q56CX9, Q7XU78, Q7XUL0, Q9SC65

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	October 3, 2006
Last modified:	November 16, 2011
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program