Reviewed, UniProtKB/Swiss-Prot Q0J930 (GUN13_ORYSJ)

Names and origin

Protein names

Recommended name:
    Endoglucanase 13
    EC=3.2.1.4
Alternative name(s):
    Endo-1,4-beta glucanase 13
    OsGLU6

Gene names

Name:	GLU6
Ordered Locus Names:	Os04g0674800, LOC_Os04g57860
ORF Names:	OSJNBa0018M05.16

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	625 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Secreted By similarity.
Tissue specificity	Expressed in roots and flowers. Ref.5
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 34	34	Potential
	Chain	35 – 625	591	Endoglucanase 13		PRO_0000249290
Sites
	Active site	427	1	By similarity
	Active site	479	1	By similarity
	Active site	488	1	By similarity
Amino acid modifications
	Glycosylation	440	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q0J930-1 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: ED817D88518F62B5	FASTA	625	68,454
	10 20 30 40 50 60 MAATMNKTPA TTFLLIPAAA SLVLLLAAAA SVEASAFDYA GAFDKCLLFF EAQRSGKLPD 70 80 90 100 110 120 DRLVRWRGDS ALTDGFSQGV DLVGGYYDSG DHVKFGLPMA YAVTMLSWGV VEFEKEMVDG 130 140 150 160 170 180 NKLHRVLDAI RWGTNYFVKA HTQHNALWVQ VGDGDSDHLC WERAEDMSTP RTAFKIDINN 190 200 210 220 230 240 PGSEVAGETA AALAAAAKAF KPYDRMYSDL LLLHSKQLFT FADTFRGKYD DSLQSAKKFY 250 260 270 280 290 300 PSASGYQDEL LWAAAWLYEA TGDEQYLRYV SQNAEAFGGT GWAVTEFSWD NKYAGLQVLL 310 320 330 340 350 360 SKVLFEQGGS AAGYADTLKQ YQAKAEFFLC ACLQKNNGHN VKMTPGGLMY VSDWSNMQYV 370 380 390 400 410 420 SSSAFLLTVY ADYLAESRGT LRCPDGEVKP AEILRFARSQ VDYVLGKNPK GMSYMVGYGS 430 440 450 460 470 480 YYPTHVHHRG ASIPSIYAMN ATVGCMESFD KYYNSKNADP NVLHGALVGG PDANDAYDDD 490 500 510 520 530 540 RCNYQHAEPT LAGNAPMSGV FARLAASPAD NTPEYTPAPN APSPSNGGSP LEFVHTVTNT 550 560 570 580 590 600 WKANGVDYYR HVVTAKNTCG HAITYLKLQI KELSGEIYGV SRTNAKDMYE FPSWMTRLDA 610 620 GAQLTIVYIQ GGPAAKIAVV EYKTA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of rice chromosome 4." Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y., Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q., Zhang L. , Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T., Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R., Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G., Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C., Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J., Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J., Li J., Hong G., Xue Y., Han B. Nature 420:316-320(2002) [PubMed: 12447439] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[5]	"OsGLU1, a putative membrane-bound endo-1,4-beta-D-glucanase from rice, affects plant internode elongation." Zhou H.-L., He S.-J., Cao Y.-R., Chen T., Du B.-X., Chu C.-C., Zhang J.-S., Chen S.-Y. Plant Mol. Biol. 60:137-151(2006) [PubMed: 16463105] [Abstract] Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL606457 Genomic DNA. Translation: CAE03241.2. AP008210 Genomic DNA. Translation: BAF16157.1. AK119913 mRNA. No translation available.
RefSeq	NP_001054243.1.
UniGene	Os.50125
3D structure databases
HSSP	HSSP built from PDB template 1IA6 based on UniProtKB Q9EYQ2.
ModBase	Search...
Protein family/group databases
CAZy	CBM49. Carbohydrate-Binding Module Family 49. GH9. Glycoside Hydrolase Family 9.
Genome annotation databases
GeneID	4337384.
KEGG	osa:4337384.
Organism-specific databases
Gramene	Q7XQ92.
Family and domain databases
InterPro	IPR012341. 6hp_glycosidase. IPR019028. CBM_49. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view]
Gene3D	G3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit.
PANTHER	PTHR22298:SF3. Glyco_hydro_9. 1 hit.
Pfam	PF09478. CBM49. 1 hit. PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. False negative. PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN13_ORYSJ

Accession

Primary (citable) accession number: Q0J930
Secondary accession number(s): Q259H9, Q7XQ92

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	October 3, 2006
Last modified:	June 16, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)