ID   SCRK2_ORYSJ             Reviewed;         336 AA.
AC   Q0J8G4; Q7XXS9; Q944F5;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2006, sequence version 1.
DT   04-NOV-2008, entry version 19.
DE   RecName: Full=Fructokinase-2;
DE            EC=2.7.1.4;
DE   AltName: Full=Fructokinase II;
DE   AltName: Full=OsFKII;
GN   Name=FRK2; Synonyms=FKII;
GN   OrderedLocusNames=Os08g0113100, LOC_Os08g02120;
GN   ORFNames=P0498H04.29, OsJ_024778;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade;
OC   Ehrhartoideae; Oryzeae; Oryza.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Panicle;
RX   PubMed=15659629; DOI=10.1105/tpc.104.028456;
RA   Moriguchi K., Suzuki T., Ito Y., Yamazaki Y., Niwa Y., Kurata N.;
RT   "Functional isolation of novel nuclear proteins showing a variety of
RT   subnuclear localizations.";
RL   Plant Cell 17:389-403(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=17210932; DOI=10.1101/gr.5509507;
RG   The rice annotation project (RAP);
RT   "Curated genome annotation of Oryza sativa ssp. japonica and
RT   comparative genome analysis with Arabidopsis thaliana.";
RL   Genome Res. 17:175-183(2007).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H.,
RA   Cong L., Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J.,
RA   Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X.,
RA   Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y.,
RA   Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J.,
RA   Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y.,
RA   Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y.,
RA   Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z.,
RA   Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T.,
RA   Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H.,
RA   Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W.,
RA   Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L.,
RA   Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J.,
RA   Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [5]
RP   PROTEIN SEQUENCE [LARGE SCALE ANALYSIS] OF 3-9, AND MASS SPECTROMETRY.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16758443; DOI=10.1002/pmic.200600043;
RA   Nozu Y., Tsugita A., Kamijo K.;
RT   "Proteomic analysis of rice leaf, stem and root tissues during growth
RT   course.";
RL   Proteomics 6:3665-3670(2006).
RN   [6]
RP   PROTEIN SEQUENCE OF 4-13; 118-127 AND 214-223, AND TISSUE SPECIFICITY.
RC   STRAIN=cv. Nipponbare; TISSUE=Anther, Panicle, Sheath, and Stem;
RX   PubMed=14681440; DOI=10.1093/nar/gkh020;
RA   Komatsu S., Kojima K., Suzuki K., Ozaki K., Higo K.;
RT   "Rice proteome database based on two-dimensional polyacrylamide gel
RT   electrophoresis: its status in 2003.";
RL   Nucleic Acids Res. 32:D388-D392(2004).
RN   [7]
RP   BIOPHYSICOCHEMICAL PROPERTIES.
RX   MEDLINE=22364004; PubMed=12475618; DOI=10.1016/S0031-9422(02)00428-4;
RA   Jiang H., Dian W., Liu F., Wu P.;
RT   "Isolation and characterization of two fructokinase cDNA clones from
RT   rice.";
RL   Phytochemistry 62:47-52(2003).
CC   -!- FUNCTION: May play an important role in maintaining the flux of
CC       carbon towards starch formation in endosperm. May also be involved
CC       in a sugar-sensing pathway.
CC   -!- CATALYTIC ACTIVITY: ATP + D-fructose = ADP + D-fructose 6-
CC       phosphate.
CC   -!- ENZYME REGULATION: Strongly inhibited at high fructose
CC       concentration.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=0.34 mM for fructose;
CC   -!- PATHWAY: Glycan biosynthesis; starch biosynthesis.
CC   -!- TISSUE SPECIFICITY: Expressed in stem, sheaths, anthers, and
CC       panicles (at protein level).
CC   -!- SIMILARITY: Belongs to the carbohydrate kinase pfkB family.
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DR   EMBL; AB110164; BAC78556.1; -; mRNA.
DR   EMBL; AP004566; BAD09515.1; -; Genomic_DNA.
DR   EMBL; AP008214; BAF22751.1; -; Genomic_DNA.
DR   EMBL; CM000145; EAZ41295.1; -; Genomic_DNA.
DR   RefSeq; NP_001060837.1; -.
DR   UniGene; Os.12780; -.
DR   GeneID; 4344497; -.
DR   KEGG; osa:4344497; -.
DR   Gramene; Q944F5; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0008865; F:fructokinase activity; IEA:EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR011611; Carb/pur_kinase.
DR   InterPro; IPR002173; Carboh/pur_kinase_PfkB_CS.
DR   Pfam; PF00294; PfkB; 1.
DR   PROSITE; PS00583; PFKB_KINASES_1; 1.
DR   PROSITE; PS00584; PFKB_KINASES_2; FALSE_NEG.
PE   1: Evidence at protein level;
KW   ATP-binding; Carbohydrate metabolism; Direct protein sequencing;
KW   Kinase; Nucleotide-binding; Transferase.
FT   CHAIN         1    336       Fructokinase-2.
FT                                /FTId=PRO_0000234065.
FT   COMPBIAS      8     14       Poly-Ala.
SQ   SEQUENCE   336 AA;  35517 MW;  FFC33297A2AC1A92 CRC64;
     MAPLGDGAAA AAAAEPNLVV SFGEMLIDFV PDVAGVSLAE SGGFVKAPGG APANVACAIS
     KLGGSSAFVG KFGDDEFGHM LVDILKKNGV NAEGCLFDEH ARTALAFVTL KSNGEREFMF
     YRNPSADMLL TEAELNLDLI RRAKIFHYGS ISLITEPCRS AHVAAMRAAK SAGILCSYDP
     NVRLPLWPSE DAARAGILSI WKEADFIKVS DDEVAFLTQG DANDEKNVLS LWFDGLKLLI
     VTDGEKGCRY FTKDFKGSVP GFSVNTVDTT GAGDAFVGSL LVNVAKDDSI FHNEEKLREA
     LKFSNACGAI CTTKKGAIPA LPTVAVAQEL ISKAAN
//