Reviewed, UniProtKB/Swiss-Prot Q0J8G4 (SCRK2_ORYSJ)

Names and origin

Protein names

Recommended name:
    Fructokinase-2
    EC=2.7.1.4
Alternative name(s):
    Fructokinase II
    OsFKII

Gene names

Name:	FRK2
Synonyms:	FKII
Ordered Locus Names:	Os08g0113100, LOC_Os08g02120
ORF Names:	P0498H04.29, OsJ_024778

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May play an important role in maintaining the flux of carbon towards starch formation in endosperm. May also be involved in a sugar-sensing pathway.
Catalytic activity	ATP + D-fructose = ADP + D-fructose 6-phosphate.
Enzyme regulation	Strongly inhibited at high fructose concentration.
Pathway	Glycan biosynthesis; starch biosynthesis.
Tissue specificity	Expressed in stem, sheaths, anthers, and panicles (at protein level). Ref.6
Sequence similarities	Belongs to the carbohydrate kinase pfkB family.
Biophysicochemical properties	Kinetic parameters: KM=0.34 mM for fructose

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW fructokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	Fructokinase-2		PRO_0000234065
Regions
	Compositional bias	8 – 14	7	Poly-Ala

Sequences

	Sequence		Length	Mass (Da)
	Q0J8G4-1 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: FFC33297A2AC1A92	FASTA	336	35,517
	10 20 30 40 50 60 MAPLGDGAAA AAAAEPNLVV SFGEMLIDFV PDVAGVSLAE SGGFVKAPGG APANVACAIS 70 80 90 100 110 120 KLGGSSAFVG KFGDDEFGHM LVDILKKNGV NAEGCLFDEH ARTALAFVTL KSNGEREFMF 130 140 150 160 170 180 YRNPSADMLL TEAELNLDLI RRAKIFHYGS ISLITEPCRS AHVAAMRAAK SAGILCSYDP 190 200 210 220 230 240 NVRLPLWPSE DAARAGILSI WKEADFIKVS DDEVAFLTQG DANDEKNVLS LWFDGLKLLI 250 260 270 280 290 300 VTDGEKGCRY FTKDFKGSVP GFSVNTVDTT GAGDAFVGSL LVNVAKDDSI FHNEEKLREA 310 320 330 LKFSNACGAI CTTKKGAIPA LPTVAVAQEL ISKAAN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Functional isolation of novel nuclear proteins showing a variety of subnuclear localizations." Moriguchi K., Suzuki T., Ito Y., Yamazaki Y., Niwa Y., Kurata N. Plant Cell 17:389-403(2005) [PubMed: 15659629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Panicle.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[5]	"Proteomic analysis of rice leaf, stem and root tissues during growth course." Nozu Y., Tsugita A., Kamijo K. Proteomics 6:3665-3670(2006) [PubMed: 16758443] [Abstract] Cited for: PROTEIN SEQUENCE [LARGE SCALE ANALYSIS] OF 3-9, MASS SPECTROMETRY. Strain: cv. Nipponbare.
[6]	"Rice proteome database based on two-dimensional polyacrylamide gel electrophoresis: its status in 2003." Komatsu S., Kojima K., Suzuki K., Ozaki K., Higo K. Nucleic Acids Res. 32:D388-D392(2004) [PubMed: 14681440] [Abstract] Cited for: PROTEIN SEQUENCE OF 4-13; 118-127 AND 214-223, TISSUE SPECIFICITY. Strain: cv. Nipponbare. Tissue: Anther, Panicle, Sheath and Stem.
[7]	"Isolation and characterization of two fructokinase cDNA clones from rice." Jiang H., Dian W., Liu F., Wu P. Phytochemistry 62:47-52(2003) [PubMed: 12475618] [Abstract] Cited for: BIOPHYSICOCHEMICAL PROPERTIES.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB110164 mRNA. Translation: BAC78556.1. AP004566 Genomic DNA. Translation: BAD09515.1. AP008214 Genomic DNA. Translation: BAF22751.1. CM000145 Genomic DNA. Translation: EAZ41295.1.
RefSeq	NP_001060837.1.
UniGene	Os.12780
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4344497.
KEGG	osa:4344497.
Organism-specific databases
Gramene	Q944F5.
Family and domain databases
InterPro	IPR011611. Carb/pur_kinase. IPR002173. Carboh/pur_kinase_PfkB_CS. [Graphical view]
Pfam	PF00294. PfkB. 1 hit. [Graphical view]
PROSITE	PS00583. PFKB_KINASES_1. 1 hit. PS00584. PFKB_KINASES_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SCRK2_ORYSJ

Accession

Primary (citable) accession number: Q0J8G4
Secondary accession number(s): Q7XXS9, Q944F5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	October 3, 2006
Last modified:	November 4, 2008
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)