Reviewed,
UniProtKB/Swiss-Prot Q0J8A4 (G3PC_ORYSJ)
Last modified
November 25, 2008.
Version 22.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Glyceraldehyde-3-phosphate dehydrogenase, cytosolic EC=1.2.1.12 Alternative name(s): PP38 | ||||||||
| Gene names |
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| Organism | Oryza sativa subsp. japonica (Rice) | ||||||||
| Taxonomic identifier | 39947 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza |
Protein attributes
| Sequence length | 337 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Catalytic activity | D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH. |
| Pathway | Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. |
| Subunit structure | Homotetramer. |
| Subcellular location | |
| Induction | By gibberellin. |
| Post-translational modification | Phosphorylated after gibberellin treatment. |
| Miscellaneous | Plants contain three forms of GAPDH: a cytosolic form which participates in glycolysis and two chloroplast forms which participates in photosynthesis. These three forms are encoded by distinct genes. |
| Sequence similarities | Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. |
Ontologies
Keywords | |
|---|---|
| Biological process | Glycolysis |
| Cellular component | Cytoplasm |
| Ligand | NAD |
| Molecular function | Oxidoreductase |
| Technical term | Direct protein sequencing |
Gene Ontology (GO) | |
| Biological process | glycolysis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | NAD binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed | ||||||
| Chain | 2 – 337 | 336 | Glyceraldehyde-3-phosphate dehydrogenase, cytosolic | PRO_0000145609 | |||||
Regions | |||||||||
| Nucleotide binding | 13 – 14 | 2 | NAD By similarity | ||||||
| Region | 153 – 155 | 3 | Glyceraldehyde 3-phosphate binding By similarity | ||||||
| Region | 213 – 214 | 2 | Glyceraldehyde 3-phosphate binding By similarity | ||||||
Sites | |||||||||
| Active site | 154 | 1 | Nucleophile By similarity | ||||||
| Binding site | 35 | 1 | NAD By similarity | ||||||
| Binding site | 82 | 1 | NAD; via carbonyl oxygen By similarity | ||||||
| Binding site | 184 | 1 | Glyceraldehyde 3-phosphate By similarity | ||||||
| Binding site | 236 | 1 | Glyceraldehyde 3-phosphate By similarity | ||||||
| Binding site | 318 | 1 | NAD By similarity | ||||||
| Site | 181 | 1 | Activates thiol group during catalysis By similarity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Molecular cloning of glyceraldehyde-3-phosphate dehydrogenase genes in rice seeds." Yoon U.H., Kim Y.H. Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Ilpoombyeo. Tissue: Seed. |
| [2] | "The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare. |
| [3] | "Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare. |
| [4] | "The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. Yang H.PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare. |
| [5] | "Rice proteome database based on two-dimensional polyacrylamide gel electrophoresis: its status in 2003." Komatsu S., Kojima K., Suzuki K., Ozaki K., Higo K. Nucleic Acids Res. 32:D388-D392(2004) [PubMed: 14681440] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-11; 30-39 AND 140-149. Strain: cv. Nipponbare. Tissue: Anther, Callus, Embryo, Panicle and Stem. |
| [6] | "Identification of phosphoproteins regulated by gibberellin in rice leaf sheath." Khan M.M.K., Jan A., Karibe H., Komatsu S. Plant Mol. Biol. 58:27-40(2005) [PubMed: 16028114] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INDUCTION, PHOSPHORYLATION. Strain: cv. Nipponbare. |
Cross-references
Sequence databases | |
|---|---|
| EF122472 mRNA. Translation: ABL74559.1. AP003886 Genomic DNA. Translation: BAD08850.1. AP008214 Genomic DNA. Translation: BAF22811.1. CM000145 Genomic DNA. Translation: EAZ41375.1. | |
| RefSeq | NP_001060897.1. |
| UniGene | Os.12168 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1IHX based on UniProtKB P56649. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 4344564. |
| KEGG | osa:4344564. |
Organism-specific databases | |
| Gramene | Q42977. |
Family and domain databases | |
| InterPro | IPR000173. GlycerAld_3-P_DHase. IPR006424. Glyceraldehyde-3-P_DHase_1. [Graphical view] |
| PANTHER | PTHR10836. GAP_DH. 1 hit. |
| Pfam | PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view] |
| PIRSF | PIRSF000149. GAP_DH. 1 hit. |
| PRINTS | PR00078. G3PDHDRGNASE. |
| TIGRFAMs | TIGR01534. GAPDH-I. 1 hit. |
| PROSITE | PS00071. GAPDH. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | G3PC_ORYSJ | ||||||||
| Accession | Primary (citable) accession number: Q0J8A4 Secondary accession number(s): Q42977, Q6ZK60 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Oryza sativa (rice) Index of Oryza sativa entries and their corresponding gene designations |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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