Reviewed, UniProtKB/Swiss-Prot Q0J8A4 (G3PC_ORYSJ)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase, cytosolic
    EC=1.2.1.12
Alternative name(s):
    PP38

Gene names

Name:	GAPC
Synonyms:	GPC
Ordered Locus Names:	Os08g0126300, LOC_Os08g03290
ORF Names:	OJ1163_G08.15, OsJ_024858

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm.
Induction	By gibberellin.
Post-translational modification	Phosphorylated after gibberellin treatment.
Miscellaneous	Plants contain three forms of GAPDH: a cytosolic form which participates in glycolysis and two chloroplast forms which participates in photosynthesis. These three forms are encoded by distinct genes.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 337	336	Glyceraldehyde-3-phosphate dehydrogenase, cytosolic		PRO_0000145609
Regions
	Nucleotide binding	13 – 14	2	NAD By similarity
	Region	153 – 155	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	213 – 214	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	154	1	Nucleophile By similarity
	Binding site	35	1	NAD By similarity
	Binding site	82	1	NAD; via carbonyl oxygen By similarity
	Binding site	184	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	236	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	318	1	NAD By similarity
	Site	181	1	Activates thiol group during catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0J8A4-1 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: 3A8154CF7A06A603	FASTA	337	36,413
	10 20 30 40 50 60 MGKIKIGING FGRIGRLVAR VALQSEDVEL VAVNDPFITT DYMTYMFKYD TVHGQWKHSD 70 80 90 100 110 120 IKIKDSKTLL LGEKPVTVFG IRNPDEIPWA EAGAEYVVES TGVFTDKEKA AAHLKGGAKK 130 140 150 160 170 180 VVISAPSKDA PMFVCGVNED KYTSDIDIVS NASCTTNCLA PLAKVIHDNF GIIEGLMTTV 190 200 210 220 230 240 HAITATQKTV DGPSSKDWRG GRAASFNIIP SSTGAAKAVG KVLPDLNGKL TGMSFRVPTV 250 260 270 280 290 300 DVSVVDLTVR IEKAASYDAI KSAIKSASEG KLKGIIGYVE EDLVSTDFVG DSRSSIFDAK 310 320 330 AGIALNDNFV KLVAWYDNEW GYSNRVIDLI RHMAKTQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of glyceraldehyde-3-phosphate dehydrogenase genes in rice seeds." Yoon U.H., Kim Y.H. Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Ilpoombyeo. Tissue: Seed.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[5]	"Rice proteome database based on two-dimensional polyacrylamide gel electrophoresis: its status in 2003." Komatsu S., Kojima K., Suzuki K., Ozaki K., Higo K. Nucleic Acids Res. 32:D388-D392(2004) [PubMed: 14681440] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-11; 30-39 AND 140-149. Strain: cv. Nipponbare. Tissue: Anther, Callus, Embryo, Panicle and Stem.
[6]	"Identification of phosphoproteins regulated by gibberellin in rice leaf sheath." Khan M.M.K., Jan A., Karibe H., Komatsu S. Plant Mol. Biol. 58:27-40(2005) [PubMed: 16028114] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INDUCTION, PHOSPHORYLATION. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	EF122472 mRNA. Translation: ABL74559.1. AP003886 Genomic DNA. Translation: BAD08850.1. AP008214 Genomic DNA. Translation: BAF22811.1. CM000145 Genomic DNA. Translation: EAZ41375.1.
RefSeq	NP_001060897.1.
UniGene	Os.12168
3D structure databases
HSSP	HSSP built from PDB template 1IHX based on UniProtKB P56649.
ModBase	Search...
Genome annotation databases
GeneID	4344564.
KEGG	osa:4344564.
Organism-specific databases
Gramene	Q42977.
Family and domain databases
InterPro	IPR000173. GlycerAld_3-P_DHase. IPR006424. Glyceraldehyde-3-P_DHase_1. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3PC_ORYSJ

Accession

Primary (citable) accession number: Q0J8A4
Secondary accession number(s): Q42977, Q6ZK60

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	October 3, 2006
Last modified:	November 25, 2008
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)