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Protein

Alpha-amylase

Gene

Os08g0473600

Organism
Oryza sativa subsp. japonica (Rice)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.UniRule annotation

Cofactori

Ca2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei204NucleophileUniRule annotation1
Active sitei229Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidaseUniRule annotation, Hydrolase
Biological processCarbohydrate metabolismUniRule annotation

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylaseUniRule annotation (EC:3.2.1.1UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolaseUniRule annotation
Gene namesi
Ordered Locus Names:Os08g0473600Imported
ORF Names:OsJ_27648Imported
OrganismiOryza sativa subsp. japonica (Rice)Imported
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25UniRule annotationAdd BLAST25
ChainiPRO_501183592826 – 437Alpha-amylaseUniRule annotationAdd BLAST412

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 370AamyInterPro annotationAdd BLAST345
Domaini365 – 425Alpha-amyl_C2InterPro annotationAdd BLAST61

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.UniRule annotation

Keywords - Domaini

SignalUniRule annotation

Phylogenomic databases

eggNOGiKOG0471. Eukaryota.
COG0366. LUCA.
KOiK01176.
OMAiYANQWAD.
OrthoDBiEOG093600FS.

Family and domain databases

InterProiView protein in InterPro
IPR012850. A-amylase_bs_C.
IPR013775. A-amylase_pln.
IPR006046. Alpha_amylase.
IPR006047. Glyco_hydro_13_cat_dom.
IPR017853. Glycoside_hydrolase_SF.
PfamiView protein in Pfam
PF07821. Alpha-amyl_C2. 1 hit.
PF00128. Alpha-amylase. 1 hit.
PIRSFiPIRSF001028. Alph-amls_plant. 1 hit.
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiView protein in SMART
SM00642. Aamy. 1 hit.
SM00810. Alpha-amyl_C2. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0J528-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKHHVTLCC VVFAVLCLAS SLAQAQVLFQ GFNWESWRKQ GGWYNFLHEK
60 70 80 90 100
VEEIASTGAT HVWLPPPSHS VSPQGYMPGR LYDLDASKYG TEAELKSLIE
110 120 130 140 150
AFHDKNVECL ADIVINHRCA DYKDSRGVYC VFEGGTPDGR LDWGPDMICS
160 170 180 190 200
DDTQYSNGRG HRDTGAGFGA APDIDHLNPR VQRELTDWLN WLRTDLGFDG
210 220 230 240 250
WRLDFAKGYS APLARIYVDN TNPTFVVGEI WSSLIYNGDG KPSTNQDADR
260 270 280 290 300
QELVNWVEGV GKPATAFDFT TKGILQAAVQ GELWRLHDGN GKAPGLMGWM
310 320 330 340 350
PDQAVTFVDN HDTGSTQSLW PFPSDKVMQG YAYILTHPGI PCIFYDHVFD
360 370 380 390 400
WNLQHEIATL AEIRSRNGIH AESTLDILKA EGDIYVAMID GKVITKLGPR
410 420 430
YDAGGIIPSD FHVVAHGNDY CVWEKEGLRV PAGRKHY
Length:437
Mass (Da):48,708
Last modified:October 3, 2006 - v1
Checksum:iC0E8276CCEA16602
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU837245 mRNA. Translation: ACF60463.1.
AP008214 Genomic DNA. Translation: BAF23937.1.
AK103413 mRNA. Translation: BAG96068.1.
CM000145 Genomic DNA. Translation: EAZ43059.1.
RefSeqiXP_015650698.1. XM_015795212.1.
UniGeneiOs.12593.

Genome annotation databases

EnsemblPlantsiOS08T0473600-01; OS08T0473600-01; OS08G0473600.
GeneIDi4345812.
GrameneiOS08T0473600-01; OS08T0473600-01; OS08G0473600.
KEGGiosa:4345812.

Similar proteinsi

Entry informationi

Entry nameiQ0J528_ORYSJ
AccessioniPrimary (citable) accession number: Q0J528
Entry historyiIntegrated into UniProtKB/TrEMBL: October 3, 2006
Last sequence update: October 3, 2006
Last modified: January 31, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)