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Q0J4I1 (CKB21_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclin-dependent kinase B2-1

Short name=CDKB2;1
Short name=CDKB;2
EC=2.7.11.22
EC=2.7.11.23
Alternative name(s):
CDC2Os-3
Gene names
Name:CDKB2-1
Synonyms:SS224
Ordered Locus Names:Os08g0512600, LOC_Os08g40170
ORF Names:P0711H09.13
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length326 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Forms a complex with CYCB2-1 or CYCB2-2 that activates CDK kinase in tobacco BY2 cells during G2/M (mitosis) phases. May be involved in the regulation of the cell cycle at the G2/M transition. Ref.6 Ref.7

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Subunit structure

Interacts with CYCB2-1 and CYCB2-2. Binding to CYCB2-1 or CYCB2-2 activates CDK kinase. Ref.7

Subcellular location

Nucleus. Cytoplasmcytoskeletonspindle. Cytoplasmcytoskeletonphragmoplast. Note: Associated to mitotic spindle during metaphase, and progressively localized the phragmoplast during telophase. Ref.7

Tissue specificity

Expressed in the dividing region of the root apex and the intercalary meristem of internodes. Ref.5 Ref.6

Developmental stage

Expressed in the G2/M phases. Ref.5 Ref.6

Induction

By cytokinin, gibberellin (GA3) and submergence. Down-regulated by the replication blocking agent hydroxyurea. Ref.5 Ref.6 Ref.8

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Sequence caution

The sequence BAA19553.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAD10065.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Mitosis
   Cellular componentCytoplasm
Cytoskeleton
Nucleus
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processhistone phosphorylation

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

hormone-mediated signaling pathway

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

mitosis

Inferred from electronic annotation. Source: UniProtKB-KW

protein autophosphorylation

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

regulation of G2/M transition of mitotic cell cycle

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

regulation of meristem structural organization

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Cellular_componentcyclin-dependent protein kinase holoenzyme complex

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

phragmoplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

spindle

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA polymerase II carboxy-terminal domain kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

cyclin-dependent protein serine/threonine kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 326326Cyclin-dependent kinase B2-1
PRO_0000296099

Regions

Domain28 – 318291Protein kinase
Nucleotide binding34 – 429ATP By similarity

Sites

Active site1591Proton acceptor By similarity
Binding site571ATP By similarity

Amino acid modifications

Modified residue381Phosphothreonine By similarity
Modified residue391Phosphotyrosine By similarity
Modified residue1931Phosphothreonine By similarity

Experimental info

Sequence conflict291E → G in AK059682. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q0J4I1 [UniParc].

Last modified October 3, 2006. Version 1.
Checksum: DC539454B81481EE

FASTA32636,977
        10         20         30         40         50         60 
MAALHHQAAA APVTTTTDGG ELRAMDLYEK LEKVGEGTYG KVYKAREKAT GRIVALKKTR 

        70         80         90        100        110        120 
LPEDDEGVPP TALREVSLLR MLSQDSHVVR LLDLKQGQNK EGQTILYLVF EYMDTDLKKF 

       130        140        150        160        170        180 
IRAHRQNLQK IPVPTVKILM YQLCKGVAFC HGRGVLHRDL KPHNLLMDRK TMALKIADLG 

       190        200        210        220        230        240 
LSRSFTVPLK KYTHEILTLW YRAPEVLLGA AHYSTPVDIW SVGCIFAELA TNQPLFAGDS 

       250        260        270        280        290        300 
EVQQLLHIFK LLGTPNEQVW PGVSKLPNWH EYPQWNPSKV SDLVHGLDAD ALDLLEKMLQ 

       310        320 
YEPSKRISAK KAMEHPYFND VNKELY 

« Hide

References

« Hide 'large scale' references
[1]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[2]"The rice annotation project database (RAP-DB): 2008 update."
The rice annotation project (RAP)
Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[3]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Nipponbare.
[4]"Nucleotide sequence of rice (oryza sativa L.) cDNA homologous to cdc2 gene."
Kidou S., Umeda M., Uchimiya H.
DNA Seq. 5:125-129(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-326.
Tissue: Callus.
[5]"Differential expression of genes for cyclin-dependent protein kinases in rice plants."
Umeda M., Umeda-Hara C., Yamaguchi M., Hashimoto J., Uchimiya H.
Plant Physiol. 119:31-40(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
[6]"The cell cycle genes cycA1;1 and cdc2Os-3 are coordinately regulated by gibberellin in planta."
Fabian T., Lorbiecke R., Umeda M., Sauter M.
Planta 211:376-383(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
[7]"Cell cycle function of a rice B2-type cyclin interacting with a B-type cyclin-dependent kinase."
Lee J., Das A., Yamaguchi M., Hashimoto J., Tsutsumi N., Uchimiya H., Umeda M.
Plant J. 34:417-425(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CYCB2-1 AND CYCB2-2.
[8]"Genome-wide identification and expression analysis of rice cell cycle genes."
Guo J., Song J., Wang F., Zhang X.S.
Plant Mol. Biol. 64:349-360(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION, GENE FAMILY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP004765 Genomic DNA. Translation: BAD10065.1. Different initiation.
AP008214 Genomic DNA. Translation: BAF24134.1.
AK059682 mRNA. No translation available.
D64036 mRNA. Translation: BAA19553.1. Different initiation.
PIRT04109.
RefSeqNP_001062220.1. NM_001068755.1.
UniGeneOs.5060.

3D structure databases

ProteinModelPortalQ0J4I1.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS08T0512600-01; OS08T0512600-01; OS08G0512600.
GeneID4346016.
KEGGosa:4346016.

Organism-specific databases

GrameneQ0J4I1.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233024.
KOK07760.
OMADWHEYPQ.
ProtClustDBCLSN2679448.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCKB21_ORYSJ
AccessionPrimary (citable) accession number: Q0J4I1
Secondary accession number(s): Q40734, Q6Z8N6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: October 3, 2006
Last modified: April 16, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations