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Q0J4I1

- CKB21_ORYSJ

UniProt

Q0J4I1 - CKB21_ORYSJ

Protein

Cyclin-dependent kinase B2-1

Gene

CDKB2-1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (03 Oct 2006)
      Previous versions | rss
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    Functioni

    Forms a complex with CYCB2-1 or CYCB2-2 that activates CDK kinase in tobacco BY2 cells during G2/M (mitosis) phases. May be involved in the regulation of the cell cycle at the G2/M transition.2 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.
    ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei57 – 571ATPPROSITE-ProRule annotation
    Active sitei159 – 1591Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi34 – 429ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC
    3. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. histone phosphorylation Source: EnsemblPlants/Gramene
    2. hormone-mediated signaling pathway Source: EnsemblPlants/Gramene
    3. mitotic nuclear division Source: UniProtKB-KW
    4. protein autophosphorylation Source: EnsemblPlants/Gramene
    5. regulation of G2/M transition of mitotic cell cycle Source: EnsemblPlants/Gramene
    6. regulation of meristem structural organization Source: EnsemblPlants/Gramene

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclin-dependent kinase B2-1 (EC:2.7.11.22, EC:2.7.11.23)
    Short name:
    CDKB2;1
    Short name:
    CDKB;2
    Alternative name(s):
    CDC2Os-3
    Gene namesi
    Name:CDKB2-1
    Synonyms:SS224
    Ordered Locus Names:Os08g0512600, LOC_Os08g40170
    ORF Names:P0711H09.13
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 8

    Organism-specific databases

    GrameneiQ0J4I1.

    Subcellular locationi

    Nucleus 1 Publication. Cytoplasmcytoskeletonspindle 1 Publication. Cytoplasmcytoskeletonphragmoplast 1 Publication
    Note: Associated to mitotic spindle during metaphase, and progressively localized the phragmoplast during telophase.

    GO - Cellular componenti

    1. cyclin-dependent protein kinase holoenzyme complex Source: EnsemblPlants/Gramene
    2. nucleus Source: UniProtKB-SubCell
    3. phragmoplast Source: UniProtKB-SubCell
    4. spindle Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 326326Cyclin-dependent kinase B2-1PRO_0000296099Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei38 – 381PhosphothreonineBy similarity
    Modified residuei39 – 391PhosphotyrosineBy similarity
    Modified residuei193 – 1931PhosphothreonineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Tissue specificityi

    Expressed in the dividing region of the root apex and the intercalary meristem of internodes.2 Publications

    Developmental stagei

    Expressed in the G2/M phases.2 Publications

    Inductioni

    By cytokinin, gibberellin (GA3) and submergence. Down-regulated by the replication blocking agent hydroxyurea.3 Publications

    Interactioni

    Subunit structurei

    Interacts with CYCB2-1 and CYCB2-2. Binding to CYCB2-1 or CYCB2-2 activates CDK kinase.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ0J4I1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini28 – 318291Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233024.
    KOiK07760.
    OMAiKATGMIV.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q0J4I1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAALHHQAAA APVTTTTDGG ELRAMDLYEK LEKVGEGTYG KVYKAREKAT    50
    GRIVALKKTR LPEDDEGVPP TALREVSLLR MLSQDSHVVR LLDLKQGQNK 100
    EGQTILYLVF EYMDTDLKKF IRAHRQNLQK IPVPTVKILM YQLCKGVAFC 150
    HGRGVLHRDL KPHNLLMDRK TMALKIADLG LSRSFTVPLK KYTHEILTLW 200
    YRAPEVLLGA AHYSTPVDIW SVGCIFAELA TNQPLFAGDS EVQQLLHIFK 250
    LLGTPNEQVW PGVSKLPNWH EYPQWNPSKV SDLVHGLDAD ALDLLEKMLQ 300
    YEPSKRISAK KAMEHPYFND VNKELY 326
    Length:326
    Mass (Da):36,977
    Last modified:October 3, 2006 - v1
    Checksum:iDC539454B81481EE
    GO

    Sequence cautioni

    The sequence BAA19553.1 differs from that shown. Reason: Erroneous initiation.
    The sequence BAD10065.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti29 – 291E → G in AK059682. (PubMed:12869764)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP004765 Genomic DNA. Translation: BAD10065.1. Different initiation.
    AP008214 Genomic DNA. Translation: BAF24134.1.
    AK059682 mRNA. No translation available.
    D64036 mRNA. Translation: BAA19553.1. Different initiation.
    PIRiT04109.
    RefSeqiNP_001062220.1. NM_001068755.1.
    UniGeneiOs.5060.

    Genome annotation databases

    EnsemblPlantsiOS08T0512600-01; OS08T0512600-01; OS08G0512600.
    GeneIDi4346016.
    KEGGiosa:4346016.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP004765 Genomic DNA. Translation: BAD10065.1 . Different initiation.
    AP008214 Genomic DNA. Translation: BAF24134.1 .
    AK059682 mRNA. No translation available.
    D64036 mRNA. Translation: BAA19553.1 . Different initiation.
    PIRi T04109.
    RefSeqi NP_001062220.1. NM_001068755.1.
    UniGenei Os.5060.

    3D structure databases

    ProteinModelPortali Q0J4I1.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS08T0512600-01 ; OS08T0512600-01 ; OS08G0512600 .
    GeneIDi 4346016.
    KEGGi osa:4346016.

    Organism-specific databases

    Gramenei Q0J4I1.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233024.
    KOi K07760.
    OMAi KATGMIV.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    2. "The rice annotation project database (RAP-DB): 2008 update."
      The rice annotation project (RAP)
      Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: cv. Nipponbare.
    3. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
      The rice full-length cDNA consortium
      Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Nipponbare.
    4. "Nucleotide sequence of rice (oryza sativa L.) cDNA homologous to cdc2 gene."
      Kidou S., Umeda M., Uchimiya H.
      DNA Seq. 5:125-129(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-326.
      Tissue: Callus.
    5. "Differential expression of genes for cyclin-dependent protein kinases in rice plants."
      Umeda M., Umeda-Hara C., Yamaguchi M., Hashimoto J., Uchimiya H.
      Plant Physiol. 119:31-40(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
    6. "The cell cycle genes cycA1;1 and cdc2Os-3 are coordinately regulated by gibberellin in planta."
      Fabian T., Lorbiecke R., Umeda M., Sauter M.
      Planta 211:376-383(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
    7. "Cell cycle function of a rice B2-type cyclin interacting with a B-type cyclin-dependent kinase."
      Lee J., Das A., Yamaguchi M., Hashimoto J., Tsutsumi N., Uchimiya H., Umeda M.
      Plant J. 34:417-425(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CYCB2-1 AND CYCB2-2.
    8. "Genome-wide identification and expression analysis of rice cell cycle genes."
      Guo J., Song J., Wang F., Zhang X.S.
      Plant Mol. Biol. 64:349-360(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION, GENE FAMILY.

    Entry informationi

    Entry nameiCKB21_ORYSJ
    AccessioniPrimary (citable) accession number: Q0J4I1
    Secondary accession number(s): Q40734, Q6Z8N6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 24, 2007
    Last sequence update: October 3, 2006
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3