Q0J4I1 (CKB21_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyclin-dependent kinase B2-1
Short name=CDKB2;1
Short name=CDKB;2
EC=2.7.11.22
EC=2.7.11.23

Alternative name(s):
CDC2Os-3

Gene names

Name:	CDKB2-1
Synonyms:	SS224
Ordered Locus Names:	Os08g0512600, LOC_Os08g40170
ORF Names:	P0711H09.13

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Forms a complex with CYCB2-1 or CYCB2-2 that activates CDK kinase in tobacco BY2 cells during G2/M (mitosis) phases. May be involved in the regulation of the cell cycle at the G2/M transition. Ref.6 Ref.7
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Subunit structure	Interacts with CYCB2-1 and CYCB2-2. Binding to CYCB2-1 or CYCB2-2 activates CDK kinase. Ref.7
Subcellular location	Nucleus. Cytoplasm › cytoskeleton › spindle. Cytoplasm › cytoskeleton › phragmoplast. Note: Associated to mitotic spindle during metaphase, and progressively localized the phragmoplast during telophase. Ref.7
Tissue specificity	Expressed in the dividing region of the root apex and the intercalary meristem of internodes. Ref.5 Ref.6
Developmental stage	Expressed in the G2/M phases. Ref.5 Ref.6
Induction	By cytokinin, gibberellin (GA3) and submergence. Down-regulated by the replication blocking agent hydroxyurea. Ref.5 Ref.6 Ref.8
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence BAA19553.1 differs from that shown. Reason: Erroneous initiation. The sequence BAD10065.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm Cytoskeleton Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW histone phosphorylation Inferred from electronic annotation. Source: EnsemblPlants/Gramene hormone-mediated signaling pathway Inferred from electronic annotation. Source: EnsemblPlants/Gramene mitosis Inferred from electronic annotation. Source: UniProtKB-KW regulation of G2/M transition of mitotic cell cycle Inferred from electronic annotation. Source: EnsemblPlants/Gramene regulation of meristem structural organization Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Cellular_component	cyclin-dependent protein kinase holoenzyme complex Inferred from electronic annotation. Source: EnsemblPlants/Gramene nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell phragmoplast Inferred from electronic annotation. Source: UniProtKB-SubCell spindle Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	Cyclin-dependent kinase B2-1		PRO_0000296099
Regions
	Domain	28 – 318	291	Protein kinase
	Nucleotide binding	34 – 42	9	ATP By similarity
Sites
	Active site	159	1	Proton acceptor By similarity
	Binding site	57	1	ATP By similarity
Amino acid modifications
	Modified residue	38	1	Phosphothreonine By similarity
	Modified residue	39	1	Phosphotyrosine By similarity
	Modified residue	193	1	Phosphothreonine By similarity
Experimental info
	Sequence conflict	29	1	E → G in AK059682. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q0J4I1 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: DC539454B81481EE	FASTA	326	36,977
	10 20 30 40 50 60 MAALHHQAAA APVTTTTDGG ELRAMDLYEK LEKVGEGTYG KVYKAREKAT GRIVALKKTR 70 80 90 100 110 120 LPEDDEGVPP TALREVSLLR MLSQDSHVVR LLDLKQGQNK EGQTILYLVF EYMDTDLKKF 130 140 150 160 170 180 IRAHRQNLQK IPVPTVKILM YQLCKGVAFC HGRGVLHRDL KPHNLLMDRK TMALKIADLG 190 200 210 220 230 240 LSRSFTVPLK KYTHEILTLW YRAPEVLLGA AHYSTPVDIW SVGCIFAELA TNQPLFAGDS 250 260 270 280 290 300 EVQQLLHIFK LLGTPNEQVW PGVSKLPNWH EYPQWNPSKV SDLVHGLDAD ALDLLEKMLQ 310 320 YEPSKRISAK KAMEHPYFND VNKELY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[3]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[4]	"Nucleotide sequence of rice (oryza sativa L.) cDNA homologous to cdc2 gene." Kidou S., Umeda M., Uchimiya H. DNA Seq. 5:125-129(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-326. Tissue: Callus.
[5]	"Differential expression of genes for cyclin-dependent protein kinases in rice plants." Umeda M., Umeda-Hara C., Yamaguchi M., Hashimoto J., Uchimiya H. Plant Physiol. 119:31-40(1999) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
[6]	"The cell cycle genes cycA1;1 and cdc2Os-3 are coordinately regulated by gibberellin in planta." Fabian T., Lorbiecke R., Umeda M., Sauter M. Planta 211:376-383(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
[7]	"Cell cycle function of a rice B2-type cyclin interacting with a B-type cyclin-dependent kinase." Lee J., Das A., Yamaguchi M., Hashimoto J., Tsutsumi N., Uchimiya H., Umeda M. Plant J. 34:417-425(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CYCB2-1 AND CYCB2-2.
[8]	"Genome-wide identification and expression analysis of rice cell cycle genes." Guo J., Song J., Wang F., Zhang X.S. Plant Mol. Biol. 64:349-360(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION, GENE FAMILY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP004765 Genomic DNA. Translation: BAD10065.1. Different initiation. AP008214 Genomic DNA. Translation: BAF24134.1. AK059682 mRNA. No translation available. D64036 mRNA. Translation: BAA19553.1. Different initiation.
PIR	T04109.
RefSeq	NP_001062220.1. NM_001068755.1.
UniGene	Os.5060.
3D structure databases
ProteinModelPortal	Q0J4I1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os08g40170.1; LOC_Os08g40170.1; LOC_Os08g40170.
GeneID	4346016.
KEGG	dosa:Os05t0483801-00. dosa:Os08t0512600-01. osa:4346016.
Organism-specific databases
Gramene	Q0J4I1.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000233024.
KO	K00924.
OMA	VSAMEAF.
ProtClustDB	CLSN2679448.
Gene expression databases
ArrayExpress	Q0J4I1.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CKB21_ORYSJ

Accession

Primary (citable) accession number: Q0J4I1
Secondary accession number(s): Q40734, Q6Z8N6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	October 3, 2006
Last modified:	May 1, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program