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Q0J4I1

- CKB21_ORYSJ

UniProt

Q0J4I1 - CKB21_ORYSJ

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Protein

Cyclin-dependent kinase B2-1

Gene

CDKB2-1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Forms a complex with CYCB2-1 or CYCB2-2 that activates CDK kinase in tobacco BY2 cells during G2/M (mitosis) phases. May be involved in the regulation of the cell cycle at the G2/M transition.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei57 – 571ATPPROSITE-ProRule annotation
Active sitei159 – 1591Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi34 – 429ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC
  3. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. mitotic nuclear division Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase B2-1 (EC:2.7.11.22, EC:2.7.11.23)
Short name:
CDKB2;1
Short name:
CDKB;2
Alternative name(s):
CDC2Os-3
Gene namesi
Name:CDKB2-1
Synonyms:SS224
Ordered Locus Names:Os08g0512600, LOC_Os08g40170
ORF Names:P0711H09.13
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 8

Organism-specific databases

GrameneiQ0J4I1.

Subcellular locationi

Nucleus 1 Publication. Cytoplasmcytoskeletonspindle 1 Publication. Cytoplasmcytoskeletonphragmoplast 1 Publication
Note: Associated to mitotic spindle during metaphase, and progressively localized the phragmoplast during telophase.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. cytoskeleton Source: UniProtKB-KW
  3. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 326326Cyclin-dependent kinase B2-1PRO_0000296099Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei38 – 381PhosphothreonineBy similarity
Modified residuei39 – 391PhosphotyrosineBy similarity
Modified residuei193 – 1931PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Expressioni

Tissue specificityi

Expressed in the dividing region of the root apex and the intercalary meristem of internodes.2 Publications

Developmental stagei

Expressed in the G2/M phases.2 Publications

Inductioni

By cytokinin, gibberellin (GA3) and submergence. Down-regulated by the replication blocking agent hydroxyurea.3 Publications

Gene expression databases

ExpressionAtlasiQ0J4I1. baseline.

Interactioni

Subunit structurei

Interacts with CYCB2-1 and CYCB2-2. Binding to CYCB2-1 or CYCB2-2 activates CDK kinase.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ0J4I1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 318291Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
InParanoidiQ0J4I1.
KOiK07760.
OMAiKATGMIV.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0J4I1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAALHHQAAA APVTTTTDGG ELRAMDLYEK LEKVGEGTYG KVYKAREKAT
60 70 80 90 100
GRIVALKKTR LPEDDEGVPP TALREVSLLR MLSQDSHVVR LLDLKQGQNK
110 120 130 140 150
EGQTILYLVF EYMDTDLKKF IRAHRQNLQK IPVPTVKILM YQLCKGVAFC
160 170 180 190 200
HGRGVLHRDL KPHNLLMDRK TMALKIADLG LSRSFTVPLK KYTHEILTLW
210 220 230 240 250
YRAPEVLLGA AHYSTPVDIW SVGCIFAELA TNQPLFAGDS EVQQLLHIFK
260 270 280 290 300
LLGTPNEQVW PGVSKLPNWH EYPQWNPSKV SDLVHGLDAD ALDLLEKMLQ
310 320
YEPSKRISAK KAMEHPYFND VNKELY
Length:326
Mass (Da):36,977
Last modified:October 3, 2006 - v1
Checksum:iDC539454B81481EE
GO

Sequence cautioni

The sequence BAA19553.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAD10065.1 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti29 – 291E → G in AK059682. (PubMed:12869764)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP004765 Genomic DNA. Translation: BAD10065.1. Different initiation.
AP008214 Genomic DNA. Translation: BAF24134.1.
AK059682 mRNA. No translation available.
D64036 mRNA. Translation: BAA19553.1. Different initiation.
PIRiT04109.
RefSeqiNP_001062220.1. NM_001068755.1.
UniGeneiOs.5060.

Genome annotation databases

EnsemblPlantsiOS08T0512600-01; OS08T0512600-01; OS08G0512600.
GeneIDi4346016.
KEGGiosa:4346016.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP004765 Genomic DNA. Translation: BAD10065.1 . Different initiation.
AP008214 Genomic DNA. Translation: BAF24134.1 .
AK059682 mRNA. No translation available.
D64036 mRNA. Translation: BAA19553.1 . Different initiation.
PIRi T04109.
RefSeqi NP_001062220.1. NM_001068755.1.
UniGenei Os.5060.

3D structure databases

ProteinModelPortali Q0J4I1.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS08T0512600-01 ; OS08T0512600-01 ; OS08G0512600 .
GeneIDi 4346016.
KEGGi osa:4346016.

Organism-specific databases

Gramenei Q0J4I1.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000233024.
InParanoidi Q0J4I1.
KOi K07760.
OMAi KATGMIV.

Gene expression databases

ExpressionAtlasi Q0J4I1. baseline.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  3. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.
  4. "Nucleotide sequence of rice (oryza sativa L.) cDNA homologous to cdc2 gene."
    Kidou S., Umeda M., Uchimiya H.
    DNA Seq. 5:125-129(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-326.
    Tissue: Callus.
  5. "Differential expression of genes for cyclin-dependent protein kinases in rice plants."
    Umeda M., Umeda-Hara C., Yamaguchi M., Hashimoto J., Uchimiya H.
    Plant Physiol. 119:31-40(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
  6. "The cell cycle genes cycA1;1 and cdc2Os-3 are coordinately regulated by gibberellin in planta."
    Fabian T., Lorbiecke R., Umeda M., Sauter M.
    Planta 211:376-383(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
  7. "Cell cycle function of a rice B2-type cyclin interacting with a B-type cyclin-dependent kinase."
    Lee J., Das A., Yamaguchi M., Hashimoto J., Tsutsumi N., Uchimiya H., Umeda M.
    Plant J. 34:417-425(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CYCB2-1 AND CYCB2-2.
  8. "Genome-wide identification and expression analysis of rice cell cycle genes."
    Guo J., Song J., Wang F., Zhang X.S.
    Plant Mol. Biol. 64:349-360(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, GENE FAMILY.

Entry informationi

Entry nameiCKB21_ORYSJ
AccessioniPrimary (citable) accession number: Q0J4I1
Secondary accession number(s): Q40734, Q6Z8N6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: October 3, 2006
Last modified: October 29, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3