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Q0J0N4 (BGL30_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-glucosidase 30

Short name=Os9bglu30
EC=3.2.1.21
Gene names
Name:BGLU30
Ordered Locus Names:Os09g0491100, LOC_Os09g31430
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length500 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-glucosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q0J0N4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q0J0N4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     377-436: EFTPIFFNYP...GHRIEFHSKH → VSVSSSSTPR...SSSTTPSRMG
     437-500: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 500475Beta-glucosidase 30
PRO_0000390347

Regions

Region463 – 4642Substrate binding By similarity

Sites

Active site1951Proton donor By similarity
Active site4091Nucleophile By similarity
Binding site461Substrate By similarity
Binding site1491Substrate By similarity
Binding site1941Substrate By similarity
Binding site3381Substrate By similarity
Binding site4561Substrate By similarity

Amino acid modifications

Glycosylation631N-linked (GlcNAc...) Potential
Glycosylation1141N-linked (GlcNAc...) Potential
Glycosylation3631N-linked (GlcNAc...) Potential
Glycosylation4161N-linked (GlcNAc...) Potential
Glycosylation4171N-linked (GlcNAc...) Potential
Disulfide bond214 ↔ 222 By similarity

Natural variations

Alternative sequence377 – 43660EFTPI…FHSKH → VSVSSSSTPRGDTTTRPSML QKTASMRVTTAHCQRRSRMD TGSSSTQSTCSSSTTPSRMG in isoform 2.
VSP_038512
Alternative sequence437 – 50064Missing in isoform 2.
VSP_038513

Experimental info

Sequence conflict1911T → F in AAL14713. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 3, 2006. Version 1.
Checksum: 1909568E1D9EFFD1

FASTA50057,388
        10         20         30         40         50         60 
MGIRMGRRLL FTLFLGALFC NGVYAKFTRY SFPKDFIFGT GSAAYQYEGA YKEGGKGPSV 

        70         80         90        100        110        120 
WDNFTHIPGK ILNNDNGDVA NDFYHRYKED VSLLKDMNMD AFRFSIAWTR ILPNGSLSGG 

       130        140        150        160        170        180 
INKEGVAFYN SLINDVIAKG MIPFVTIFHW DTPLALESKY GGFLSEDIVK EYVDFAEVCF 

       190        200        210        220        230        240 
REFGDRVKYW TTFNEPFTYS AYGYGKGVFA PGRCSSYVSK SCGVGDSSRE PYLVAHHIHL 

       250        260        270        280        290        300 
SHAAAVQLYR TKYQPTQKGQ IGMVVVTHWF VPYDNSDADR GAVQRSLDFI YGWFMDPIVH 

       310        320        330        340        350        360 
GDYPGTMRGW LGNRLPEFTP EQSAMVKGSY DFIGVNYYTT YYAKSIPPPN SNELSYDLDN 

       370        380        390        400        410        420 
RANTTGFRNG KPIGPQEFTP IFFNYPPGLR ELLLYTKRRY NNPTIYVTEN GIDEGNNSTL 

       430        440        450        460        470        480 
PEALKDGHRI EFHSKHLQFV NHAIKNGVNV KGYFTWTFMD CFEWGDGYLD RFGLIYVDRK 

       490        500 
TLKRYRKESS YWIEDFLKRH 

« Hide

Isoform 2 [UniParc].

Checksum: EF36799CC8700D9B
Show »

FASTA43648,782

References

« Hide 'large scale' references
[1]"Beta-glucosidase isozyme expression in rice."
Opassiri R., Ketudat Cairns J.R., Vichitphan S., Esen A.
Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Orion.
[2]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"The rice annotation project database (RAP-DB): 2008 update."
The rice annotation project (RAP)
Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[4]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 135-500 (ISOFORM 2).
Strain: cv. Nipponbare.
[5]"Analysis of rice glycosyl hydrolase family 1 and expression of Os4bglu12 beta-glucosidase."
Opassiri R., Pomthong B., Onkoksoong T., Akiyama T., Esen A., Ketudat Cairns J.R.
BMC Plant Biol. 6:33-33(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY056828 mRNA. Translation: AAL14713.1.
AP008215 Genomic DNA. Translation: BAF25453.1.
AK061340 mRNA. No translation available.
RefSeqNP_001063539.1. NM_001070074.1. [Q0J0N4-1]
UniGeneOs.81365.

3D structure databases

ProteinModelPortalQ0J0N4.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH1. Glycoside Hydrolase Family 1.

Proteomic databases

PRIDEQ0J0N4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS09T0491100-01; OS09T0491100-01; OS09G0491100. [Q0J0N4-1]
GeneID4347441.
KEGGosa:4347441.

Organism-specific databases

GrameneQ0J0N4.

Phylogenomic databases

eggNOGCOG2723.
KOK01188.
OMACAFYCAN.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10353. PTHR10353. 1 hit.
PfamPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGL30_ORYSJ
AccessionPrimary (citable) accession number: Q0J0N4
Secondary accession number(s): Q93ZK6
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: October 3, 2006
Last modified: May 14, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries