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Q0J0G2 (BGL32_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-glucosidase 32

Short name=Os9bglu32
EC=3.2.1.21
Gene names
Name:BGLU32
Ordered Locus Names:Os09g0511700, LOC_Os09g33690
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length508 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-glucosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q0J0G2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q0J0G2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     256-274: AIQGGQIGITLLGWWYEPY → LATGNSRRSDRDHSAGLVV
     275-508: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3030 Potential
Chain31 – 508478Beta-glucosidase 32
PRO_0000390349

Regions

Region458 – 4592Substrate binding By similarity

Sites

Active site1961Proton donor By similarity
Active site4041Nucleophile By similarity
Binding site501Substrate By similarity
Binding site1501Substrate By similarity
Binding site1951Substrate By similarity
Binding site3391Substrate By similarity
Binding site4511Substrate By similarity

Amino acid modifications

Glycosylation2221N-linked (GlcNAc...) Potential
Glycosylation4411N-linked (GlcNAc...) Potential
Disulfide bond215 ↔ 223 By similarity

Natural variations

Alternative sequence256 – 27419AIQGG…WYEPY → LATGNSRRSDRDHSAGLVV in isoform 2.
VSP_038514
Alternative sequence275 – 508234Missing in isoform 2.
VSP_038515

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 15, 2009. Version 2.
Checksum: 52256C1319709A6C

FASTA50856,799
        10         20         30         40         50         60 
MAAGARALVP SPFIVVVFLL LAAAARDASA LTRHDFPEGF VFGAGTSAFQ VEGAAAEDGR 

        70         80         90        100        110        120 
KPSIWDTFTH QGYSPGGAIA DVSADQYHHY KEDVKLMYDM GLDAYRFSIA WPRLIPDGRG 

       130        140        150        160        170        180 
EINPKGLEYY NNLIDELIMH GIQPHVTIYH FDLPQALQDE YGGILSPRFI EDYTAYAEVC 

       190        200        210        220        230        240 
FKNFGDRVKH WVTVNEPNIE PIGGYDAGVQ PPRRCSYPFG TNCTGGDSST EPYIVAHHLL 

       250        260        270        280        290        300 
LAHASAVSIY RQKYQAIQGG QIGITLLGWW YEPYTDAVAD AAAAIRMNEF HIGWFMNPLV 

       310        320        330        340        350        360 
HGDYPPVMRS RVGARLPSIT ASDSEKIRGS FDFIGINHYF VIFVQSSDAN HDQKLRDYYV 

       370        380        390        400        410        420 
DAGVQENGGG GFDKEHYQLH PWALGKMLHH LKLKYGNPPV MIHENGDADS PETPGKIDYD 

       430        440        450        460        470        480 
DDFRSDFLQS YLEVLHLSIR NGSNTRGYFV WSLLDGFEFL SGYGNRFGLC CVDFTAPART 

       490        500 
RYVRSSARWY SDFLNGGELR PVKPFVAL 

« Hide

Isoform 2 [UniParc].

Checksum: 33E8AFDE306C8926
Show »

FASTA27430,212

References

« Hide 'large scale' references
[1]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[2]"The rice annotation project database (RAP-DB): 2008 update."
The rice annotation project (RAP)
Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[3]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: cv. Nipponbare.
[4]"Analysis of rice glycosyl hydrolase family 1 and expression of Os4bglu12 beta-glucosidase."
Opassiri R., Pomthong B., Onkoksoong T., Akiyama T., Esen A., Ketudat Cairns J.R.
BMC Plant Biol. 6:33-33(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP008215 Genomic DNA. Translation: BAF25553.1.
AK101420 mRNA. Translation: BAG95059.1.
RefSeqNP_001063639.1. NM_001070174.1.
UniGeneOs.17433.

3D structure databases

ProteinModelPortalQ0J0G2.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH1. Glycoside Hydrolase Family 1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4347546.
KEGGosa:4347546.

Organism-specific databases

GrameneQ0J0G2.

Phylogenomic databases

eggNOGCOG2723.
HOGENOMHOG000088630.

Gene expression databases

ArrayExpressQ0J0G2.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10353. PTHR10353. 1 hit.
PfamPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGL32_ORYSJ
AccessionPrimary (citable) accession number: Q0J0G2
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: December 15, 2009
Last modified: February 19, 2014
This is version 51 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries