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Protein

Proteasome subunit alpha type-7-B

Gene

PAD1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.PROSITE-ProRule annotation

GO - Molecular functioni

  1. threonine-type endopeptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiREACT_283342. APC/C:Cdc20 mediated degradation of Securin.
REACT_292166. Autodegradation of the E3 ubiquitin ligase COP1.
REACT_295447. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
REACT_300539. Hedgehog 'on' state.
REACT_301524. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_311865. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_319722. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_330023. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_333718. Separation of Sister Chromatids.
REACT_336348. CDK-mediated phosphorylation and removal of Cdc6.
REACT_340130. ER-Phagosome pathway.
REACT_344832. Orc1 removal from chromatin.
REACT_346168. Autodegradation of Cdh1 by Cdh1:APC/C.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit alpha type-7-B (EC:3.4.25.1)
Alternative name(s):
20S proteasome alpha subunit D-2
20S proteasome subunit alpha-4-B
Gene namesi
Name:PAD1
Ordered Locus Names:Os09g0538200, LOC_Os09g36710
ORF Names:OsJ_028982, P0229B10.22, P0569E11.44
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763 Componenti: Chromosome 9

Organism-specific databases

GrameneiQ0J006.

Subcellular locationi

  1. Cytoplasm By similarity
  2. Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
  3. proteasome core complex, alpha-subunit complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 249249Proteasome subunit alpha type-7-BPRO_0000124163Add
BLAST

Proteomic databases

PaxDbiQ0J006.
PRIDEiQ0J006.

Expressioni

Gene expression databases

ExpressionAtlasiQ0J006. baseline.

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.

Structurei

3D structure databases

ProteinModelPortaliQ0J006.
SMRiQ0J006. Positions 4-231.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1A family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0638.
InParanoidiQ0J006.
KOiK02731.
OMAiKGPAKET.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000426. Proteasome_asu_N.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view]
SMARTiSM00948. Proteasome_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0J006-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARYDRAITV FSPDGHLFQV EYALEAVRKG NAAVGVRGSD TVVLGVEKKS
60 70 80 90 100
TPKLQDSRSV RKIASLDTHI ALACAGLKAD ARVLINRARV ECQSHRLTVE
110 120 130 140 150
DAVTVEYITR YIAGLQQKYT QSGGVRPFGL STLIVGFDPY TDKPALYQTD
160 170 180 190 200
PSGTFSAWKA NATGRNSNSM REFLEKNYKE TSGKETIKLA IRALLEVVES
210 220 230 240
GGKNIEIAVM TQKDGLRQLE EAEIDEYVAE IEAEKAAAEA AKKGAPKET
Length:249
Mass (Da):27,214
Last modified:October 3, 2006 - v1
Checksum:iD20D0B27143D976E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti85 – 851Missing in BAA99540 (PubMed:10854779).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB032061 mRNA. Translation: BAA99540.1.
AP006067 Genomic DNA. Translation: BAD34241.1.
AP006174 Genomic DNA. Translation: BAD34378.1.
AP008215 Genomic DNA. Translation: BAF25709.1.
CM000146 Genomic DNA. Translation: EAZ45499.1.
RefSeqiNP_001063795.1. NM_001070330.1.
UniGeneiOs.3737.

Genome annotation databases

EnsemblPlantsiOS09T0538200-01; OS09T0538200-01; OS09G0538200.
GeneIDi4347710.
KEGGiosa:4347710.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB032061 mRNA. Translation: BAA99540.1.
AP006067 Genomic DNA. Translation: BAD34241.1.
AP006174 Genomic DNA. Translation: BAD34378.1.
AP008215 Genomic DNA. Translation: BAF25709.1.
CM000146 Genomic DNA. Translation: EAZ45499.1.
RefSeqiNP_001063795.1. NM_001070330.1.
UniGeneiOs.3737.

3D structure databases

ProteinModelPortaliQ0J006.
SMRiQ0J006. Positions 4-231.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiQ0J006.
PRIDEiQ0J006.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS09T0538200-01; OS09T0538200-01; OS09G0538200.
GeneIDi4347710.
KEGGiosa:4347710.

Organism-specific databases

GrameneiQ0J006.

Phylogenomic databases

eggNOGiCOG0638.
InParanoidiQ0J006.
KOiK02731.
OMAiKGPAKET.

Enzyme and pathway databases

ReactomeiREACT_283342. APC/C:Cdc20 mediated degradation of Securin.
REACT_292166. Autodegradation of the E3 ubiquitin ligase COP1.
REACT_295447. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
REACT_300539. Hedgehog 'on' state.
REACT_301524. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_311865. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_319722. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_330023. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_333718. Separation of Sister Chromatids.
REACT_336348. CDK-mediated phosphorylation and removal of Cdc6.
REACT_340130. ER-Phagosome pathway.
REACT_344832. Orc1 removal from chromatin.
REACT_346168. Autodegradation of Cdh1 by Cdh1:APC/C.

Gene expression databases

ExpressionAtlasiQ0J006. baseline.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000426. Proteasome_asu_N.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view]
SMARTiSM00948. Proteasome_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Primary structural features of the 20S proteasome subunits of rice (Oryza sativa)."
    Sassa H., Oguchi S., Inoue T., Hirano H.
    Gene 250:61-66(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Nipponbare.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  4. "The genomes of Oryza sativa: a history of duplications."
    Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
    , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
    PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.

Entry informationi

Entry nameiPSA7B_ORYSJ
AccessioniPrimary (citable) accession number: Q0J006
Secondary accession number(s): O04861, Q69JF0, Q9LRI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: October 3, 2006
Last modified: April 1, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.