Reviewed, UniProtKB/Swiss-Prot Q0ITW7 (ADH2_ORYSJ)

Names and origin

Protein names

Recommended name:
    Alcohol dehydrogenase 2
    EC=1.1.1.1

Gene names

Name:	ADH2
Synonyms:	DUPR11.1
Ordered Locus Names:	Os11g0210500, LOC_Os11g10510

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	An alcohol + NAD+ = an aldehyde or ketone + NADH.
Cofactor	Binds 2 zinc ions per subunit.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family.
Sequence caution	The sequence AK102799 differs from that shown. Reason: Frameshift at position 369. The sequence BAF27848.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alcohol dehydrogenase (NAD) activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 379	379	Alcohol dehydrogenase 2		PRO_0000160708
Regions
	Nucleotide binding	202 – 207	6	NAD By similarity
	Nucleotide binding	295 – 297	3	NAD By similarity
Sites
	Metal binding	47	1	Zinc 1; catalytic By similarity
	Metal binding	69	1	Zinc 1; catalytic By similarity
	Metal binding	99	1	Zinc 2 By similarity
	Metal binding	102	1	Zinc 2 By similarity
	Metal binding	105	1	Zinc 2 By similarity
	Metal binding	113	1	Zinc 2 By similarity
	Metal binding	177	1	Zinc 1; catalytic By similarity
	Binding site	226	1	NAD By similarity
	Binding site	231	1	NAD By similarity
	Binding site	372	1	NAD By similarity
Experimental info
	Sequence conflict	42	1	L → I in AK102799. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q0ITW7-1 [UniParc]. Last modified June 12, 2007. Version 2. Checksum: 2DF98CF6B6BEE8EA	FASTA	379	41,207
	10 20 30 40 50 60 MATAGKVIKC KAAVAWEAGK PLSIEEVEVA PPQAMEVRVK ILYTALCHTD VYFWEAKGQT 70 80 90 100 110 120 PVFPRILGHE AGGIVESVGE GVTELAPGDH VLPVFTGECK ECDHCKSEES NMCDLLRINV 130 140 150 160 170 180 DRGVMIGDGK SRFTIKGKPI FHFVGTSTFS EYTVIHVGCL AKINPEAPLD KVCILSCGFS 190 200 210 220 230 240 TGFGATVNVA KPKKGQTVAI FGLGAVGLAA MEGARLSGAS RIIGVDLNPA KFEQAKKFGC 250 260 270 280 290 300 TDFVNPKDHS KPVHEVLIEM TNGGLDRAVE CTGNINAMIS CFECVHDGWG VAVLVGVPTK 310 320 330 340 350 360 DDVFKTHPMN FLNEKTLKGT FFGNYKPRTD LPNVVELYMK KELELEKFIT HSVPFSEINT 370 AFDLMLKGES LRCVMRMDE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete sequence of 340 kb of DNA around the rice Adh1-Adh2 region reveals interrupted colinearity with maize chromosome 4." Tarchini R., Biddle P., Wineland R., Tingey S., Rafalski A. Plant Cell 12:381-391(2000) [PubMed: 10715324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications." The rice chromosomes 11 and 12 sequencing consortia BMC Biol. 3:20-20(2005) [PubMed: 16188032] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[5]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF172282 Genomic DNA. Translation: AAF34412.1. DP000010 Genomic DNA. Translation: ABA92034.1. AP008217 Genomic DNA. Translation: BAF27848.1. Sequence problems. AK102799 mRNA. No translation available.
RefSeq	NP_001067485.1.
UniGene	Os.8815
3D structure databases
SMR	Q0ITW7. Positions 4-377.
ModBase	Search...
Genome annotation databases
GeneID	4350054.
GenomeReviews	Gene locus ADH2 in contig AP008217_GR.
KEGG	osa:4350054.
Organism-specific databases
Gramene	Q2R8Z2.
Phylogenomic databases
HOGENOM	HBG753318.
Family and domain databases
InterPro	IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn. IPR013149. ADH_Zn-bd. IPR002328. ADH_Zn_CS. IPR011032. GroES-like. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADH2_ORYSJ

Accession

Primary (citable) accession number: Q0ITW7
Secondary accession number(s): P18332, Q2R8Z2, Q9LLQ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 12, 2007
Last sequence update:	June 12, 2007
Last modified:	January 19, 2010
This is version 26 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)